Dr Anjam Khan
- Email: email@example.com
- Telephone: +44 (0) 191 208 7066
- Fax: +44 (0) 191 208 7427
- Address: Microbial Pathogenicity Group
Institute for Cell and Molecular Biosciences
& School of Biomedical Sciences
The Medical School
University of Newcastle
Roles and Responsibilities
Director, Faculty of Medical Sciences Microbiology Containment Level 3 Research Suites
Chair, ICaMB Microbiological Hazards and Genetic Modification Safety Advisory Committee
Member, ICaMB Safety Committee
Advisor, University Microbiological Hazards and Genetic Modification Safety Advisory Committee
PhD in Molecular Biology, with Professor John Scaife FRSE, University of Edinburgh.
BSc Hons in Biochemistry, University of Manchester Institute of Science and Technology.
University of Cambridge, Postdoctoral Research Fellow.
Lecturer in Microbiology, University of Newcastle.
Our major research objectives are to understand the biology of Salmonella and gain insights into the mechanisms by which it causes disease. We are using a mutidisciplinary approach with an international team of collaborators to fulfill these objectives.
Quorum Sensing and Host-Pathogen Communication
Research on quorum sensing has provided vivid insights into the mechanisms by which bacteria coordinate their efforts and behave in a “multicellular” fashion. We are investigating how Salmonella use small signal molecules called autoinducers to communicate with each other, and aim at identifying the genes and physiological processes regulated by quorum sensing. Strikingly, we have evidence revealing inter-kingdom crosstalk between pathogens and their hosts. Thus enabling Salmonella to “eavesdrop” on host communication systems by sensing neuroendocrine stress hormones and using these signals as cues to regulate the expression of genes important in virulence.
The Bacterial Cytoskeleton
Intracellular cytoskeletal protein assemblies with a cell-shape defining function have recently been discovered in bacteria. The bacterial actin-like MreB protein polymerises into helical filamentous structures which span across the cytoplasm and interact with components of the inner membrane. We are elucidating the contribution of the bacterial cytoskeleton to the biology and pathogenicity of Salmonella. We have demonstrated the MreB-based cytoskeleton is important for the expression of flagella and the Salmonella pathogenicity island 1 (SPI-1) Type3 secretion system as well as cell morphology.
Burkholderia cepacia and Inflammatory Host Responses
Burkholderia cepacia is an opportunistic pathogen of cystic fibrosis and lung transplant patients. We are investigating the proinflammatory activities of different genomovars of B.cepacia and correlating these with poor post lung transplant outcome. Our team has demonstrated that the lipopolysaccharide (LPS) chemotype is responsible for this activity. We are also identifying genes involved in the biosynthetic pathway of LPS.
The Research Group
Postdoctoral Research Associates:
Dr Michail Karavolos (MRC)
Dr David Bulmer (MRC)
Dr Sonya Carnell (Italian CF Trust)
Dr Wei Chen (China Scholarship Council)
Present and Recent Graduate PhD Students:
Ms Anne Doble (MRC)
Ms Lubna Kharraz (Ford Foundation of America)
Ms Hannah Spencer (BBSRC)
Mr Rerngwit Boonyom (Royal Thai Government)
Molecular and Cellular Biology of Microbial Pathogens
Infection and Immunity
Director of the FMS Microbiology Containment Level 3 Research Suites
Prospective International students, as well as UK students, with interests in Molecular and Cellular Microbiology are particularly encouraged to apply for doctoral degrees (PhD).
Member of the Medical Research Council (MRC) Infections and Immunity Board College of Experts
Medical Research Council (MRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Newcastle Healthcare and NHS Hospitals Special Trustees Charity
Four International Patents Awarded in the areas of Biotechnology, and Vaccine Discovery and Delivery
Undergraduate teaching include the following modules:
BMS2002: Cell Signalling
MIC2025: Bacterial Interactions with Human Hosts and
the Immune System in Human Disease
MIC2027: Parasitic and Viral Diseases (Module Leader)
MIC3043: Research in Medical Microbiology and Immunology
MIC3027: Bacterial Pathogenicity and Disease
MIC3046: Pathogenic Viruses, Protozoa, and Fungi (Module Leader)
CMB3000: Research Projects
- Karavolos MH, Winzer K, Williams P, Khan CM. Pathogen espionage: multiple bacterial adrenergic sensors eavesdrop on host communication systems. Molecular Microbiology 2013, 87(3), 455-465.
- Bulmer DM, Kharraz L, Grant AJ, Dean P, Morgan FJ, Karavolos MH, Doble AC, McGhie EJ, Koronakis V, Daniel RA, Mastroeni P, Khan CMA. The bacterial cytoskeleton modulates motility, type 3 secretion, and colonization in Salmonella. PLoS Pathogens 2012, 8(1), e1002500.
- Karavolos MH, Bulmer D, Spencer H, Rampioni G, Schmalen I, Baker S, Pickard D, Gray J, Fookes M, Winzer K, Ivens A, Dougan G, Williams P, Khan CMA. Salmonella Typhi sense host neuroendocrine stress hormones and release the toxin haemolysin E. EMBO Reports 2011, 12(3), 252-258.
- Khan CMA. The Dynamic Interactions between Salmonella and the Microbiota, within the Challenging Niche of the Gastrointestinal Tract. ISRN Microbiology 2014, 2014, 846049.
- Doble AC, Bulmer DM, Kharraz L, Karavolos MH, Khan CMA. The function of the bacterial cytoskeleton in Salmonella pathogenesis. Virulence 2012, 3(5), 446-449.
- Nicholson A, Perry JD, James AL, Stanforth SP, Carnell S, Wilkinson K, Anjam Khan CM, De Soyza A, Gould FK. In vitro activity of S-(3,4-dichlorobenzyl)isothiourea hydrochloride and novel structurally related compounds against multidrug-resistant bacteria, including Pseudomonas aeruginosa and Burkholderia cepacia complex. International Journal of Antimicrobial Agents 2012, 39(1), 27-32.
- Staniewicz L, Donald AM, Stokes DJ, Thomson N, Sivaniah E, Grant A, Bulmer D, Khan A. The Application of STEM and In situ Controlled Dehydration to Bacterial Systems Using ESEM. Scanning 2012, 34(4), 237-246.
- Karavolos MH, Williams P, Khan CMA. Interkingdom crosstalk Host neuroendocrine stress hormones drive the hemolytic behavior of Salmonella typhi. Virulence 2011, 2(4), 371-374.
- Boonyom R, Karavolos MH, Bulmer DM, Khan CM. Salmonella pathogenicity island 1 (SPI-1) type III secretion of SopD involves N- and C-terminal signals and direct binding to the InvC ATPase. Microbiology 2010, 156(6), 1805-1814.
- Spencer H, Karavolos MH, Bulmer DM, Aldridge P, Chhabra SR, Winzer K, Williams P, Khan CMA. Genome-Wide Transposon Mutagenesis Identifies a Role for Host Neuroendocrine Stress Hormones in Regulating the Expression of Virulence Genes in Salmonella. Journal of Bacteriology 2010, 192(3), 714-724.
- Karavolos MH, Khan CMA. Multidirectional Chemical Signalling Between Mammalian Hosts, Resident Microbiota, and Invasive Pathogens: Neuroendocrine Hormone-Induced Changes in Bacterial Gene Expression. In: Microbial Endocrinology: The Microbiota-Gut-Brain Axis in Health and Disease. New York: Springer, 2014, pp.241-253.
- Carnell SC, Perry JD, Khan CMA, De Soyza A. The bacterial cytoskeleton-A new antimicrobial target in cystic fibrosis pathogens?. In: Thorax: British Thoracic Society Winter Meeting. 2010, Westminster, UK: BMJ Group.
- Perrett CA, Karavolos MH, Humphrey S, Mastroeni P, Martinez-Argudo I, Spencer H, Bulmer D, Winzer K, McGhie E, Koronakis V, Williams P, Khan CMA, Jepson MA. LuxS-Based Quorum Sensing Does Not Affect the Ability of Salmonella enterica Serovar Typhimurium To Express the SPI-1 Type 3 Secretion System, Induce Membrane Ruffles, or Invade Epithelial Cells. Journal of Bacteriology 2009, 191(23), 7253-7259.
- Ierano T, Silipo A, Sturiale L, Garozzo D, Bryant C, Lanzetta R, Parrilli M, Aldridge C, Gould FK, Corris PA, Khan CMA, De Soyza A, Molinaro A. First structural characterization of Burkholderia vietnamiensis lipooligosaccharide from cystic fibrosis-associated lung transplantation strains. Glycobiology 2009, 19(11), 1214-1223.
- Karavolos MH, Spencer H, Bulmer DM, Thompson A, Winzer K, Williams P, Hinton JCD, Anjam Khan CM. Adrenaline modulates the global transcriptional profile of Salmonella revealing a role in the antimicrobial peptide and oxidative stress resistance responses. BMC Genomics 2008, 9(1), 458.
- Karavolos MH, Bulmer DM, Winzer K, Wilson M, Mastroeni P, Williams P, Khan CMA. LuxS affects flagellar phase variation independently of quorum sensing in Salmonella enterica serovar typhimurium. Journal of Bacteriology 2008, 190(2), 769-771.
- McKelvie ND, Khan SA, Karavolos MH, Bulmer DM, Lee JJ, DeMarco R, Maskell DJ, Zavala F, Hormaeche CE, Anjam Khan CM. Genetic detoxification of an aroA Salmonella enterica serovar Typhimurium vaccine strain does not compromise protection against virulent Salmonella and enhances the immune responses towards a protective malarial antigen. FEMS Immunology and Medical Microbiology 2008, 52(2), 237-246.
- Ieranò T, Silipo A, Sturiale L, Garozzo D, Brookes H, Khan CM, Bryant C, Gould FK, Corris PA, Lanzetta R, Parrilli M, De Soyza A, Molinaro A. The structure and proinflammatory activity of the lipopolysaccharide from Burkholderia multivorans and the differences between clonal strains colonizing pre and posttransplanted lungs. Glycobiology 2008, 18(11), 871-881.
- Liu W-T, Karavolos MH, Bulmer DM, Allaoui A, Hormaeche RDCE, Lee JJ, Anjam Khan CM. Role of the universal stress protein UspA of Salmonella in growth arrest, stress and virulence. Microbial Pathogenesis 2007, 42(1), 2-10.
- Silipo A, Molinaro A, Ierano T, De Soyza A, Sturiale L, Garozzo D, Aldridge C, Corris PA, Khan CMA, Lanzetta R, Parrilli M. The complete structure and pro-inflammatory activity of the lipooligosaccharide of the highly epidemic and virulent gram-negative bacterium Burkholderia cenocepacia ET-12 (strain J2315). Chemistry - A European Journal 2007, 13(12), 3501-3511.
- Karavolos MH, Roe AJ, Wilson M, Henderson J, Lee JJ, Gally DL, Khan CMA. Erratum: Type III secretion of the Salmonella effector protein SopE is mediated via an N-terminal amino acid signal and not an mRNA sequence (Journal of Bacteriology (2005) 187, 5 (1559-1567)). Journal of Bacteriology 2005, 187(15), 5505-5505.
- Karavolos MH, Wilson M, Henderson J, Lee JJ, Khan CMA. Type III secretion of the Salmonella effector protein SopE is mediated via an N-terminal amino acid signal and not an mRNA sequence. Journal of Bacteriology 2005, 187(5), 1559-1567.
- Aldridge PD, Gray MA, Hirst BH, Khan CMA. Who's talking to whom? Epithelial-bacterial pathogen interactions. In: International Symposium on Epithelial-Bacterial Pathogen Interactions. 2004, Newcastle upon Tyne, UK: Molecular Microbiology: Wiley-Blackwell.
- De Soyza A, Ellis CD, Khan CMA, Corris P, Demarco de Hormaeche R. Burkholderia Cenocepacia Lipopolysaccharide, Lipid A and Proinflammatory Activity. American Journal of Respiratory and Critical Care Medicine 2004, 170(1), 70-77.
- Ellis CD, Lindner B, Khan CMA, Zahringer U, Demarco De Hormaeche R. The Neisseria gonorrhoeae lpxLII gene encodes for a late-functioning lauroyl acyl transferase, and a null mutation within the gene has a significant effect on the induction of acute inflammatory responses. Molecular Microbiology 2001, 42(1), 167-181.
- Heal KG, McConkey GA, Hormaeche CE, Hollingdale MR, Khan CMA, Taylor-Robinson AW. Expression of TetC fusion proteins from Salmonella in a gaseous environment that models conditions found in mammalian tissues. BioTechniques 2000, 28(2), 228-232.
- McNeill HV, Sinha KA, Hormaeche CE, Lee JJ, Khan CMA. Development of a nonantibiotic dominant marker for positively selecting expression plasmids in multivalent Salmonella vaccines. Applied and Environmental Microbiology 2000, 66(3), 1216-1219.
- Lee JJ, Sinha KA, Harrison JA, Demarco De Hormaeche R, Riveau G, Pierce RJ, Capron A, Wilson RA, Khan CMA. Tetanus toxin fragment C expressed in live Salmonella vaccines enhances antibody responses to its fusion partner Schistosoma haematobium glutathione S-transferase. Infection and Immunity 2000, 68(5), 2503-2512.
- Carnell SC, Biboy J, Cerardi G, Ville B, Deleuse C, Samain R, Vollmer D, Khan CMA, Gray J, Vollmer W, De Soyza A. Pathogen associated molecular patterns in cystic fibrosis pathogens: Analysis of peptidoglycan structure. In: British Thoracic Society Winter Meeting. 2013, London, UK: BMJ Publishing Group.
- Macfarlane JG, Ruchaud-Sparagano MH, Scott JA, Bulmer DA, Khan CMA, Simpson AJ. Src Kinase Inhibition Attenuates Neutrophil Degranulation Without Impairing Bacterial Killing: A Possible Therapeutic Strategy for Acute Lung Injury?. In: British Thoracic Society Winter Meeting. 2013, London, UK: BMJ Publishing Group.
- Carnell SC, Perry JD, Vollmer D, Biboy J, Facchini M, Bragonzi A, Vergunst A, Vollmer W, Khan CMA, De Soyza A. Targeting the bacterial cytoskeleton of CF pathogens for antimicrobial development – A cautionary tale?. In: British Thoracic Society Winter Meeting. 2013, London, UK: BMJ Publishing Group.
- Carnell SC, Perry JD, Borthwick L, Vollmer D, Biboy J, Facchini M, Bragonzi A, Silipo A, Vergunst AC, Vollmer W, Khan ACM, De Soyza A. Targeting the bacterial cytoskeleton of the Burkholderia cepacia complex for antimicrobial development: A cautionary tale. International Journal of Molecular Sciences 2018, 19(6), 1604.