Dr Timothy Cheek
Reader in Cell Signalling
- Email: email@example.com
- Telephone: +44 (0) 191 208 7010
- Fax: +44 (0) 191 208 7424
- Address: Institute for Cell and Molecular Biosciences
The Medical School
University of Newcastle upon Tyne
Newcastle upon Tyne
BackgroundPhD VACANCIES – we are always open to informal enquiries from prospective PhD students, whether Home/EU or non-EU. Please feel free to email me if you are interested (firstname.lastname@example.org)
Natalie Bell, BBSRC DTG Studentship (MRes/PhD)
Magomet Aushev, non-EU (MRes/PhD)
Claire Whitworth, Randerson Foundation Studentship (MRes/PhD)
Roles and Responsibilities
Postgraduate Tutor, ICaMB
BSc (Hons), Sheffield City Polytechnic (CNAA) 1984
PhD, University of Liverpool 1987
Royal Society University Research Fellow, University of Cambridge 1992-96
Junior Research Fellow, Churchill College, Cambridge 1993-96
Visiting Associate Professor, INSERM U.274 Physiologie et Pharmacologie Cellulaire, Orsay, France 1995
Official Fellow, Girton College, Cambridge 1996-98
The mechanisms and functions of intracellular Ca2+ signals. Specifically the role of Ca2+ signals in electrically excitable (neuronal) cell types.
Ca2+ signalling in neuronal development
Neurones in the brain are the result of differentiation of neuronal stem cells during development. One of the goals in modern neurobiology is to understand the molecular mechanisms controlling neuronal differentiation, as the ability to produce neurones in vitro will have enormous implications for treatment of neurological disorders and age-related neurodegenerative diseases. Stimulus-induced changes in the concentration of intracellular fee Ca2+ underlie many aspects of neuronal cell function, including differentiation, and Ca2+ signalling dysfunction has been implicated in a number neurological diseases and disorders.
Our group discovered that the switch from proliferation to differentiation in human neuroblastoma cells is accompanied by a switch in two key Ca2+ signalling pathways; one pathway (store-operated Ca2+ entry) becomes down-regulated, while there is a functional upregulation of a non-store-operated pathway.
We are using a combination of molecular, biochemical and cellular techniques to understand the molecular mechanisms that coordinate the switch in Ca2+ signalling pathways and to unravel the role that the switch plays in driving and maintaining the differentiation response.
Research in our laboratory is funded by the MRC, the BBSRC and the Wellcome Trust.
BSc (Hons) - Stage 1, Stage 2, Stage 3
BDS - Stage 1
MBBS - Stage 1, Stage 2
Accelerated MBBS - Stage 1
Postgraduate Tutor, ICaMB
- Bell N, Hann V, Redfern CPF, Cheek TR. Store-operated Ca2+ entry in proliferating and retinoic acid-differentiated N- and S-type neuroblastoma cells. Biochimica et Biophysica Acta: Molecular Cell Research 2013, 1833(3), 643-651.
- Heer R, Clarke N, Rigas AC, Cheek TR, Pickard R, Leung HY. Phenotypic Modulation of Human Urinary Tract Stroma-derived Fibroblasts by Transforming Growth Factor β3. Urology 2010, 76(2), 509.e13-509.e20.
- Sattelle DB, Cordova D, Cheek TR. Insect ryanodine receptors: Molecular targets for novel pest control chemicals. Invertebrate Neuroscience 2008, 8(3), 107-119.
- Tottey S, Waldron KJ, Firbank SJ, Reale B, Bessant C, Sato K, Cheek TR, Gray J, Banfield MJ, Dennison C, Robinson NJ. Protein-folding location can regulate manganese-binding versus copper- or zinc-binding. Nature 2008, 455(7216), 1138-1142.
- Riddoch, F.C., Brown, A.M., Rowbotham, S.E., Redfern, C.P.F., Cheek, T.R. Changes in functional properties of the caffeine-sensitive Ca2+ store during differentiation of human SH-SY5Y neuroblastoma cells. Cell Calcium 2007, 41(3), 195-206.
- Clarke N, Heer R, Cheek T, Leung T, Pickard R. Changing the phenotype of urethral stroma by treatment with transforming growth factor beta-3. European Urology Supplements 2007, 6(2), 138-138.
- Cheek TR, Thorn P. A constitutively active nonselective cation conductance underlies resting Ca2+ influx and secretion in bovine adrenal chromaffin cells. Cell Calcium 2006, 40(3), 309-318.
- Brown, A.M., Riddoch, F.C, Robson, A., Redfern, C.P.F., Cheek, T.R. Mechanistic and functional changes in Ca2+ entry after retinoic acid-induced differentiation of neuroblastoma cells. Biochemical Journal 2005, 388(3), 941-948.
- Riddoch, F.C., Rowbotham, S.E., Brown, A.M., Redfern, C.P.F., Cheek, T.R. Release and sequestration of Ca2+ by a caffeine- and ryanodine-sensitive store in a sub-population of human SH-SY5Y neuroblastoma cells. Cell Calcium 2005, 38(2), 111-120.
- Brown AM, Riddoch FC, Redfern CPF, Cheek TR. Changes in Ca2+ entry mechanisms following retinoic acid-induced differentiation of neuroblastoma cells. In: Meeting of the Physiological Society. 2002, University of Leeds, Leeds, England: Journal of Physiology: The Physiological Society.
- Whitworth CL, Redfern CPF, Cheek TR. Differentiation-induced remodelling of store-operated calcium entry is independent of neuronal or glial phenotype but modulated by cellular context. Molecular Neurobiology 2018, Epub ahead of print.
- Edwards N, Anderson CMH, Conlon NJ, Watson AK, Hall RJ, Cheek TR, Embley TM, Thwaites DT. Resculpting the binding pocket of APC superfamily LeuT-fold amino acid transporters. Cellular and Molecular Life Sciences 2018, 75(5), 921-938.
- Conlon NJ, Edwards N, Anderson CMH, Cheek TR, Thwaites DT. Rheogenic amino acid transport by Drosophila CG4991 of the Amino Acid Auxin Permease (AAAP) transporter family. FASEB Journal 2013, 27, 732-735.