Dynamic analysis of STAT6 signalling in living cells (2002)

Author(s): Nelson G, Wilde GJC, Spiller DG, Sullivan E, Unitt JF, White MRH

  • : Dynamic analysis of STAT6 signalling in living cells

Abstract: Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP-STAT6) fusion protein appeared to be more active than the C-terminal fusion. In HEK-293 cells both fusion proteins formed fluorescent nuclear foci following IL-4 stimulation, but in HeLa cells nuclear accumulation was homogeneous. Stimulation of the NF-kappaB pathway through TNFalpha treatment, or expression of p65-EGFP fusion protein, repressed both basal STAT6-dependent transcriptional activity and the extent of activation in response to IL-4. This indicates a novel mechanism of inhibition of STAT6 signalling by NF-kappaB activation. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Notes: 625CK Times Cited:5 Cited References Count:25

  • Short Title: Dynamic analysis of STAT6 signalling in living cells
  • Alternate Title: Febs Lett Febs Lett
  • Date: Dec 4
  • Secondary Title: Febs Letters
  • Volume: 532
  • Number: 1-2
  • Pages: 188-192
  • Publication type: Generic
  • Bibliographic status: Published

Keywords: stat6 nf-kappa b confocal microscopy green fluorescent protein nf-kappa-b interleukin-4-induced transcription factor jak-3 janus kinase il-4 activation proteins receptor expression mechanisms responses


Dr Glyn Nelson
Senior Research Associate