Background

Current Position

Senior Research Technician/ Lab Manager – Genomics Core Facility

Experience

I have been delivering genomic services to research and commercial settings since 2003. Did a lot of Sanger sequencing and then began Next Generation Sequencing (NGS) on Illumina’s first instrument, the Genome Analyzer IIx, which the University had from 2008 to 2013. For NGS I now use Illumina’s MiSeq, NextSeq, and the NovaSeq. I have been at the Genomics Core Facility from the beginning (2015/2016) and I am qualified on all our instrumentation. I provide specific training on the 10X Genomics platform and Laser Microdissection (LMD).

Qualifications

BSc (Hons) Biomedical Science, University of Sunderland 2000 (including one year’s work placement at MRC Building, Institute for the Health of the Elderly, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne, UK).

Previous roles

Research Technician – Mitochondrial Group of Professor Patrick Chinnery: 2014 - 2015

Senior Research Technician – BHF group of Professor Bernard Keavney: 2006 - 2013

Molecular Biology Technician – Complement Genomics: 2003 - 2005

Other interests

Active member of NU TechNet and Race Equality Charter Self Assessment Team

Key Words

Genomics / Next-Generation sequencing / 10X/ Single-cell / Transcriptomics / Spatial Gene Expression / DNA / RNA / ddPCR/ Laser Microscopy/ Whole Genomes/ Exomes/ NU TechNet/ REC SAT/

Publications

• Griffin H, McDonald DO, Singh-Dang T, Dickinson R, Grainger A, Reynard L, Hussain R, Cant AJ, Gennery AR, Abinun M, Flood TJ, Collin MP, Loughlin J, Morgan NV, Santibanez-Koref M, Hambleton S. Whole exome sequencing as a diagnostic tool in primary immunodeficiency. In: 15th Biennial Meeting European Society for Immunodeficiency (ESID). 2012, Florence, Italy: Springer.
• Dickinson RE, Griffin H, Bigley V, Reynard LN, Hussain R, Haniffa M, Lakey JH, Rahman T, Wang XN, McGovern N, Pagan S, Cookson S, McDonald D, Chua I, Wallis J, Cant A, Wright M, Keavney B, Chinnery PF, Loughlin J, Hambleton S, Santibanez-Koref M, Collin M. Exome sequencing identifies GATA-2 mutation as the cause of dendritic cell, monocyte, B and NK lymphoid deficiency. Blood 2011, 118(10), 2656-2658.
• Seifer CM, Hussain R, Clarke M, Kenny RA. Cardiovascular effects of carteolol and timolol in the treatment of glaucoma. Journal of the American Geriatrics Society 1999, 47, P321.
• Seifer CM, Brennan R, Hussain R, Clarke M, Kenny RA. Comparison of response to carotid sinus massage in elderly persons with and without raised intraocular pressure. In: Journal of the American Geriatrics Society. 1999, John Wiley & Sons, Inc.
• Collin J, Queen R, Zerti D, Dorgau B, Georgiou M, Djidrovski I, Hussain R, Coxhead JM, Joseph A, Rooney P, Lisgo S, Figueiredo FC, Armstrong L, Lako M. Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface. The Ocular Surface 2020, 19, 190-200.
• Collin J, Zerti D, Queen R, SantoFerreira T, Bauer R, Coxhead J, Hussain R, Steel D, Mellough C, Ader M, Sernagor E, Armstrong L, Lako M. CRX Expression in Pluripotent Stem Cell‐Derived Photoreceptors Marks a Transplantable Subpopulation of Early Cones. Stem Cells (Durham) 2019, 37(5), 609-622.
• Hussain R, Steel DH, Sandinha T, Stappler T, Heimann H, Wong D. Cutting the Internal Limiting Membrane With Zero Aspiration Technique: A Clinical Audit. Retina 2019, 39, S133-S136.
• Collin J, Queen R, Zerti D, Dorgau B, Hussain R, Coxhead J, Cockell S, Lako M. Deconstructing Retinal Organoids: Single cell RNA-Seq reveals the cellular components of human pluripotent stem cell-derived retina. Stem Cells (Durham) 2019, 37(5), 593-598.
• Duncan CJA, Dinnigan E, Theobald R, Grainger A, Skelton AJ, Hussain R, Willet JDP, Swan DJ, Coxhead J, Thomas MF, Thomas J, Zamvar V, Slatter MA, Cant AJ, Engelhardt KR, Hambleton S. Early-onset autoimmune disease due to a heterozygous loss-of-function mutation in TNFAIP3 (A20). Annals of the Rheumatic Diseases 2018, 77(5), 783-786.
• Coxhead J, Kurzawa-Akanbi M, Hussain R, Pyle A, Chinnery P, Hudson G. Somatic mtDNA variation is an important component of Parkinson's disease. Neurobiology of Aging 2016, 38, 217.e1-217.e6.
• Keogh MJ, Steele H, Douroudis K, Pyle A, Duff J, Hussain R, Smertenko T, Griffin H, Santibanez-Koref M, Horvath R, Chinnery PF. Frequency of rare recessive mutations in unexplained late onset cerebellar ataxia. Journal of Neurology 2015, 262(8), 1822-1827.
• Jiang Y, Habibollah S, Tilgner K, Collin J, Barta T, Al-Aama JY, Tesarov L, Hussain R, Trafford AW, Kirkwood G, Sernagor E, Eleftheriou CG, Przyborski S, Stojkovic M, Lako M, Keavney B, Armstrong L. An Induced Pluripotent Stem Cell Model of Hypoplastic Left Heart Syndrome (HLHS) Reveals Multiple Expression and Functional Differences in HLHS-Derived Cardiac Myocytes. Stem Cells Translational Medicine 2014, 3(4), 416-423.
• Bada WU, Miossec MJ, Hussain R, Goodship J, Keavney B. Investigating the cause of transposition of great arteries. In: Frontiers in CardioVascular Biology 2014: Third Congress of the ESC Council on Basic Cardiovascular Science. 2014, Barcelona: Oxford University Press.
• Bada WU, Miossec MJ, Hussain R, Rahman T, Topf A, Hall D, Santibanez-Koref M, Keavney B, Goodship J. Investigating the cause of transposition of great arteries; exome sequencing analysis. In: ESC Congress 2014. 2014, Barcelona, Spain: Oxford University Press.
• Tan HL, Topf A, Griffin H, Doza JP, Hall D, Hussain R, Eden J, Rahman T, Cunnington M, Goodship J, Keavney B. Role of Genetic Variation in the Transcriptional Inhibitor Protein SMAD6 in the Predispoition to Congenital Cardiovascular Malformation. In: British Cardiovascular Society Annual Conference and Exhibition. 2009, London, UK: BMJ Publishing Group.