Health Protection Research Unit for Chemical & Radiation Threats & Hazards

Projects

The Contribution of Cutaneous Metabolism to Local and Systemic Toxicity

A survey of the peer-reviewed literature, including research papers and case studies, will identify the extent of local and systemic toxicity resulting from cutaneous uptake and metabolism of topical compounds in occupational and environmental exposure scenarios, taking into account acute and chronic endpoints including skin sensitisation and carcinogenesis.  

In parallel, we will generate activity data from functional assays for phase I and II metabolism, using a selection of probe substrates for important enzyme activities identified from previous work, using in vitro systems (fresh ex vivo skin, HaCat cells, fresh keratinocytes, and living skin equivalents). These assays would act as ‘positive controls’ to assess metabolic competence of skin samples/cultures and provide baseline data for future research.