Institute of Cellular Medicine

Staff Profile

Dr Shruti Chachra

Research Assistant

Background

Roles and Responsibilities

Research Assistant: The Agius Group

Qualifications

PhD (2015)- Forest Biotechnology, Forest Research Institute, India
MSc (2009)- Bangalore University, India
BSc (2007)- Kurukshetra University, India
Level 3 Award in Education and Training, Newcastle College, Newcastle upon Tyne

Previous Positions

2016-2017: Lecturer, Gateshead College, Gateshead
2015- 2016: Lecturer, Newcastle College, Newcastle upon Tyne
2015- 2016: Guest Researcher, university of Newcastle, Newcastle upon Tyne
2014-2015: Senior Research Fellow, Forest Research Institute, India
2012-2014: Junior Research Fellow, Forest Research Institute, India
2011-2012: Junior Research Fellow, Forest Research Institute, India


Memberships

Diabetes UK

Teaching

Worked as the Lecturer  at:

The department of Health and Social Care, School of Health and Enterprise, Newcastle College, Newcastle upon Tyne.
The department of Health Care and Early Years, Gateshead College

 TEACHING AND LEARNING DELIVERY:

  • Delivered full range of teaching and learning activities and conducted hands on practicals for the learners in a range of subjects: Molecular Biology, Microbiology,  Biochemistry, Immunology, Plant Biology,  Human Biology

COURSE OPERATIONAL ACTIVITIES:

  • Assessed, recorded and reported on development, progress, retention and achievement of students.
  • Advised, guided and supported students to identify and achieve their learning aims and objectives with the help of  SMART targets and by providing 1:1 tutorials and feedback
  • Carried out associated preparation (preparing assignment briefs, scheme of work, lesson plans) and assessment processes (marking assignments and reports prepared by students).
  • Contributed to the enrichment activities for learners including educational trips and hands on training
  • Guided and advised on further education and future career of students

CURRICULUM ORGANIZATION:

  • Produced and revised schemes of work, lesson plans, assignment briefs and associated materials
  • Contributed to securing the quality of the curriculum in terms of  issues such as attendance, retention and achievement

 

Research

NEWCASTLE UNIVERSITY (2015 - 2016)

WorkED on the gene expression of a bacterial strain involving:

  • Isolation of RNA
  • Preparation of cDNA library
  • Designing and validation of primers
  • Optimization of annealing temperatures: gradient, end-point PCR
  • qPCR

FOREST RESEARCH INSTITUTE (2009-2015)

Has worked in the following areas of research with different forest tree species along with the execution of following programs:

  • Establishment of experimental trials: Established progeny and clonal trials of different forest tree species like Melia composita, Pongamia pinnata and Dalbergia sissoo in various states of North India

  • Collection of periodic data for analysis: Collected growth data periodically from various trials of Melia composita, Dalbergia sissoo and Pongamia pinnata established in different  states of Northern India like Punjab and Haryana to analyse the performance of various genotypes in different environment on the basis of different growth attributes like height, collar diameter, diameter at breast height, clear bole height and straightness

  • Optimization of DNA and RNA extraction protocols: Optimized protocols for the extraction of total genomic DNA and RNA from leaf foliage of Melia composita and Pongamia pinnata to obtain pure DNA and RNA for further downstream processes like amplification through polymerase chain reaction

  •  Qualitative and quantitative analysis of DNA and RNA: Quality of DNA and RNA checked by agarose gel electrophoresis and quantity was estimated using biophotometer and nanodrop

  • Designing and validation of primers for PCR, qPCR, RT-PCR using various softwares

  • Preparation of cDNA libraries from RNA

  • Amplification and resolution of PCR product: Amplification of DNA done by polymerase chain reaction in thermal cycler using dominant and co-dominant molecular markers like Random Amplification of Polymorphic DNA (RAPDs), Inter Simple Sequence Repeats (ISSRs) and Simple Sequence Repeats (SSRs). The PCR product then resolved by Agarose gel electrophoresis (AGE) and Poly acryalamide gel electrophoresis (PAGE)

  •  Analysis and interpretation of result:  Interpretation done with the help of dendrograms obtained and genetic diversity analysed of different species was analysed for applying strategies like marker assisted selection (MAS) and association mapping (AM)

Publications

  • Kumar A, Dobhal S, Sharma S. Assessment of genetic diversity in different clones of Dalbergia sissoo (Roxb.) by RAPD markers. African Journal of Biotechnology 2011, 10(35), 6686-6694.
  • Sharma S, Kumar A, Dobhal S, Rawat A. Standardization of protocol for Genomic DNA isolation in Melia composita Willd. Indian Journal of Biotechnology 2016. Submitted.
  • Sharma S, Kumar A, Dobhal S. Wastelands rehabilitation by growing genetically improved Melia composita Willd. Environment and Ecology Research 2016. Submitted.
  • Sharma S, Dobhal S, Kumar A. Optimization of DNA extraction protocol of Pongamia pinnata Linn. In: 1st Indian Forest Congress. 2011. In Preparation.
  • Kumar A, Dobhal S, Sharma S, Ahmed N, Rawat US. Genetic screening and improvement of Melia composita Willd. Annals of Forestry 2013, 21 (2), 189-196. In Preparation.
  • Kumar A, Pande PK, Ravichandran S, Naithani S, Kishore PB, Raturi A, Gautam P, Dobhal S, Sharma S. Genetic Analysis of growth and wood variations in Leucaena leucocephala (LAM.) De Wit. Journal of Forestry Research 2012, 24(3), 485-493. In Preparation.
  • Dobhal S, Kumar A, Sharma S. Genetic diversity in Dalbergia sissoo (Roxb.) through molecular markers for practical applications. The Indian Forester 2012, 138(9), 819-821. In Preparation.