Institute of Genetic Medicine

Genomic Core Facility

Genomics Core Facility

Based at the Institute of Genetic Medicine, the Genomics Core Facility (GCF) underlines Newcastle University's drive to stay at the forefront of genomics research, at both the macro and single-cell level.

Overview

The principle aim of the GCF is to support and deliver cutting-edge genomics research; from project conception and design, methodological guidance and technical resource, through to specialist single-cell bioinformatic support.

Instrumentation

As a dedicated facility, our current instrumentation set-up allows for a high level of service flexibility, enabling us to rapidly shift between genomic, transcriptomic and epigenomic sample analysis.

Next-generation sequencing   

  • Illumina NovaSeq 6000   
  • Illumina NextSeq 500 (x2)
  • Illumina MiSeq

Production scale genotyping and gene expression

  • Fluidigm BioMark HD System (qPCR)
  • BioRad QX200 Droplet Digital PCR AutoDG System (ddPCR)

Single cell isolation and sample preparation

  • Leica LMD7 (laser-capture micro-dissection)
  • 10X Genomics Chromium
  • Fluidigm C1 Single-Cell Auto Prep System

In support of sample QC (DNA and RNA) and NGS library preparation, the GCF operate an Agilent 4200 Tapestation; LabChip GX Touch; Qubit and Nano-Drop; pre and post PCR automated liquid handlers (Beckman Coulter Biomek 4000 and NXP) and a Diagenode Bioruptor Pico sonication device. 

Staff and contact details

The GCF is led by Dr Jonathan Coxhead, bringing over a decade of genomics experience, and is supported by dedicated technical and bioinformatic staff who can provide a complete genomics service, training, guidance on experimental design and data analysis. Academic lead for the facility is Prof Joris Veltman.

Please contact Dr Jonathan Coxhead (jonathan.coxhead@newcastle.ac.uk) for further details.