Dr John Taylor
Senior Lecturer in Molecular Immunology
- Email: firstname.lastname@example.org
- Telephone: +44 (0) 191 208 8694
- Address: Oral Biology
School of Dental Sciences
Newcastle upon Tyne
Roles and Responsibilities
Research group leader
Postgraduate research supervisor
BSc (Hons) Applied Biochemistry Brunel University 1981
PhD in Autoimmune Disease Studies University of Newcastle 1987
Certificate in Teaching and Learning in Higher Education 1994
British Society for Immunology
International Association for Dental Research
Association for Basic Science Teachers in Dentistry
I am interested in the immunopathogenesis of human periodontitis and characterisation of molecular biomarkers for periodontal disease management.
Cells found in the periodontium e.g. myeloid immune cells, fibroblasts and keratinocytes act as sentinel cells to initiate immune responses to microbial pathogens in chronic inflammatory disorders such as periodontitis. Thus, cytokines secreted by these cells have a critical role in regulating innate and adaptive immune responses in the periodontium and an inappropriate cytokine response leads to destructive inflammation mediated by the action of host enzymes such as matrix metalloproteinases (MMPs). Furthermore, cross-susceptibility between periodontitis and systemic disease is explained at least in part by dysregulation of cytokine networks in common conditions such as obesity and type 2 diabetes mellitus (T2DM).
The elucidation of cytokine-mediated immune responses to periodontal pathogens is therefore central to our understanding of the cellular events which lead to compromised periodontal health in a wide-range of patient groups, not just those with primary oral disease. This knowledge will enhance patient management through characterisation of rational biomarkers which may identify susceptible patients and predict the clinical course of the disease and the response to treatment. Biomarkers might be soluble mediators in oral fluids such as saliva or may be genetic variants associated with particular disease phenotypes.
My research takes place at the laboratory–clinical interface as part of the Translational Oral Biosciences group of the Centre for Oral Health Research (COHR) and the Institute of Cellular Medicine (ICM).
Leptin is a peptide hormone secreted by adipocytes (adipokine) which serves to regulate metabolism and appetite but also has an immunoregulatory function in a wide range of tissues. We hypothesise that leptin may have a synergistic effect with cytokines in signalling immune competent cells in the periodontium and that this pathway may contribute to shared susceptibility between periodontitis and conditions of hyperleptinemia (such as obesity and T2DM). We have investigated this by examining the leptin-induced signalling pathways and molecular responses in myeloid immune cells such as monocytes. It is intriguing that stromal cells such as fibroblasts are considered to be pro-active in defining tissue specific immune responses and disease pathogenesis, so a particular focus of our current research are the pathways and responses of gingival fibroblasts triggered by leptin synergising with pro-inflammatory cytokines such as IL-1.
The functional research of periodontal immune responses in vitro informs our translational research into biomarkers of periodontal disease. With the support of our industrial partners, we have conducted a number of longitudinal studies assessing the efficacy of cytokines, adipokines (such as leptin) and other inflammatory mediators in biological fluids as biomarkers. In a project funded by the EPSRC and the Technology Strategy Board we are working with 2 local biotechnology companies (OJbio and ORLA Protein Technologies) to develop novel approaches to periodontal disease monitoring. In this project we are developing and characterising a point-of-care analytical device based on proprietary nanoscale-biological technology which will give real-time information relating to disease activity which will inform patient management.
We are also building on our previous work on genetic association of cytokine gene polymorphism with periodontitis to investigate the interaction of environmental factors (e.g. smoking) with single nucleotide polymorphisms and cytokine profiles in periodontitis patients. We hope to develop a holistic framework based on genetic, immunological and clinical data which accurately defines periodontitis phenotype, disease progression and treatment response.
Fellow of the Faculty of Medical Sciences Graduate School
Current students: 2 PhD; 3 Masters
EPSRC/Technology Strategy Board
The Nuffield Foundation
The Oral and Dental Research Trust
Newcastle University Hospitals Special Trustees
Northern Regional Health Authority
Philips Oral Healthcare
- Stage 1 BDS Cell Biology
- Stage 1 BDS Dental Physiology
- Stage 2 BDS Oral Environment
- Stage 2 BDS Immunology and Health Care (Course Leader)
- Stage 3 BDS Human Diseases
BSc undergraduate project supervisor
MRes an MSc Project supervisor
MClinDent Project supervisor
- Williams RC, Skelton AJ, Todryk SM, Rowan AD, Preshaw PM, Taylor JJ. Leptin and Pro-Inflammatory Stimuli Synergistically Upregulate MMP-1 and MMP-3 Secretion in Human Gingival Fibroblasts. PLOS One 2016, 11(2), e0148024.
- Taylor JJ, Preshaw PM. Gingival crevicular fluid and saliva. Periodontology 2000 2016, 70(1), 7-10.
- Jaedicke K, Preshaw PM, Taylor JJ. Salivary cytokines as biomarkers of periodontal diseases. Periodontology 2000 2016, 70(1), 164-183.
- Birch J, Anderson RK, Correia-Melo C, Jurk D, Hewitt G, Marques FM, Green NJ, Moisey E, Birrell MA, Belvisi MG, Black F, Taylor JJ, Fisher AJ, De Soyza A, Passos JF. DNA damage response at telomeres contributes to lung aging and chronic obstructive pulmonary disease. American Journal of Physiology: Lung Cellular and Molecular Physiology 2015, 309(10), L1124-L1137.
- Jitprasertwong P, Jaedicke KM, Nile CJ, Preshaw PM, Taylor JJ. Leptin enhances the secretion of interleukin (IL)-18, but not IL-1B, from human monocytes via activation of caspase-1. Cytokine 2014, 65(2), 222-230.
- Taylor JJ. Protein Biomarkers of Periodontitis in Saliva. ISRN Inflammation 2014, 1-18.
- Taylor JJ, Preshaw PM, Lalla E. A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes. Journal of Clinical Periodontology 2013, 40(Supp.s14), S113-S134.
- Jaedicke KM, Roythorne A, Padget K, Todryk S, Preshaw PM, Taylor JJ. Leptin up-regulates TLR2 in human monocytes. Journal of Leukocyte Biology 2013, 93(4), 561-571.
- Jaedicke KJ, Taylor JJ, Preshaw PM. Validation and quality control for ELISAs for use with human saliva samples. Journal of Immunological Methods 2012, 377(1-2), 62-65.
- Preshaw PM, Taylor JJ. How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis?. Journal of Clinical Periodontology 2011, 38(s11), 60-84.
- Davies RC, Jaedicke KM, Barksby HE, Jitprasertwong P, Al-Shahwani RM, Taylor JJ, Preshaw PM. Do patients with aggressive periodontitis have evidence of diabetes? A pilot study. Journal of Periodontal Research 2011, 46(6), 663-672.
- Nile CJ, Barksby E, Jitprasertwong P, Preshaw PM, Taylor JJ. Expression and regulation of interleukin-33 in human monocytes. Immunology 2010, 130(2), 172-180.
- Taylor JJ. Cytokine regulation of immune responses to Porphyromonas gingivalis. Periodontology 2000 2010, 54(1), 160-194.
- Barksby HE, Nile CJ, Jaedicke KM, Taylor JJ, Preshaw PM. Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide. Clinical and Experimental Immunology 2009, 156(3), 479-487.
- Cullinan MP, Westerman B, Hamlet SM, Palmer JE, Faddy MJ, Seymour GJ, Middleton PG, Taylor JJ. Progression of periodontal disease and interleukin-10 gene polymorphism. Journal of Periodontal Research 2008, 43(3), 328-333.
- Preshaw PM, Foster N, Taylor JJ. Cross-susceptibility between periodontal disease and type 2 diabetes mellitus: an immunobiological perspective. Periodontology 2000 2007, 45(1), 138-157.
- Donaldson P, Veeramani S, Baragiotta A, Floreani A, Venturi C, Pearce S, Wilson V, Jones D, James O, Taylor J, Newton J, Bassendine M. Cytotoxic T-Lymphocyte-Associated Antigen-4 Single Nucleotide Polymorphisms and Haplotypes in Primary Biliary Cirrhosis. Clinical Gastroenterology and Hepatology 2007, 5(6), 755-760.
- Foster N, Lea SR, Preshaw PM, Taylor JJ. Pivotal advance: Vasoactive intestinal peptide inhibits up-regulation of human monocyte TLR2 and TLR4 by LPS and differentiation of monocytes to macrophages. Journal of Leukocyte Biology 2007, 81(4), 893-903.
- Barksby HE, Lea SR, Preshaw PM, Taylor JJ. The expanding family of interleukin-1 cytokines and their role in destructive inflammatory disorders. Clinical and Experimental Immunology 2007, 149(2), 217-225.
- Foster N, Andreadou K, Jamieson L, Preshaw PM, Taylor JJ. VIP inhibits P. gingivalis LPS-induced IL-18 and IL-18BPa in monocytes. Journal of Dental Research 2007, 86(9), 883-887.
- De Silva NT, Preshaw PM, Taylor JJ, Jayaratne SD, Heasman PA, Fernando DJS. Periodontitis: A complication of type 2 diabetes in Sri Lankans. Diabetes Research and Clinical Practice 2006, 74(2), 209-210.
- Birraux J, Kirby JA, Thomason JM, Taylor JJ. The effect of cyclosporin on cell division and apoptosis in human oral keratinocytes. Journal of Periodontal Research 2006, 41(4), 297-302.
- Pleguezuelos O, Dainty SJ, Kapas S, Taylor JJ. A human oral keratinocyte cell line responds to human heat shock protein 60 through activation of ERK1/2 MAP kinases and upregulation of IL-1β. Clinical and Experimental Immunology 2005, 141(2), 307-314.
- Foster N, Cheetham J, Taylor JJ, Preshaw PM. VIP inhibits Porphyromonas gingivalis LPS-induced immune responses in human monocytes. Journal of Dental Research 2005, 84(11), 999-1004.
- Ellis JS, Morgan CL, Kirby JA, Taylor JJ, Thomason JM. Plasma TGF-β1 as a risk factor for gingival overgrowth. Journal of Clinical Periodontology 2004, 31(10), 863-868.
- Ellis JS, Seymour RA, Taylor JJ, Thomason JM. Prevalence of gingival overgrowth in transplant patients immunosuppressed with tacrolimus. Journal of Clinical Periodontology 2004, 31(2), 126-131.
- P. M. Preshaw, D. R. Geatch, B. Lauffart, M. K. Jeffcoat, J. J. Taylor and P. A. Heasman. Longitudinal changes in TCRB variable gene expression and markers of gingival inflammation in experimental gingivitis. Journal of Clinical Periodontology 1998, 25, 774-780.
- D. R. Geatch, D. A. Ross, P. A. Heasman and J. J. Taylor. Expression of T-cell receptor V beta 2, 6 and 8 gene families in chronic adult periodontal disease. European Journal of Oral Sciences 1997, 105, 397-404.
- Taylor JJ, Preshaw PM, Donaldson PT. Cytokine gene polymorphism and immunoregulation in periodontal disease. Periodontology 2000 2004, 35(1), 158-182.
- Seymour GJ, Taylor JJ. Shouts and whispers: an introduction to immunoregulation in periodontal disease. Periodontology 2000 2004, 35(1), 9-13.
- Harris JI, Russell RRB, Curtis MA, Aduse-Opoku J, Taylor JJ. Molecular mediators of Porphyromonas gingivalis-induced T-cell apoptosis. Oral Microbiology and Immunology 2002, 17(4), 224-230.
- Parkhill JM, Hennig BJW, Chapple ILC, Heasman PA, Taylor JJ. Association of interleukin-1 gene polymorphisms with early-onset periodontitis. Journal of Clinical Periodontology 2000, 27(9), 682-689.
- Hennig BJW, Parkhill JM, Chapple ILC, Heasman PA, Taylor JJ. Dinucleotide repeat polymorphism in the interleukin-10 gene promoter (IL-10.G) and genetic susceptibility to early-onset periodontal disease. Genes and Immunity 2000, 1(6), 402-404.
- Gemmell E, Prajaneh S, Grieco DA, Taylor JJ, Seymour GJ. Apoptosis in Porphyromonas gingivalis-specific T-cell lines. Oral Microbiology and Immunology 1999, 14(6), 331-338.
- Hennig BJW, Parkhill JM, Chapple LLC, Heasman PA, Taylor JJ. Association of a vitamin D receptor gene polymorphism with localized early-onset periodontal diseases. Journal of Periodontology 1999, 70(9), 1032-1038.
- Geatch DR, Harris JI, Heasman PA, Taylor JJ. In vitro studies of lymphocyte apoptosis induced by the periodontal pathogen Porphyromonas gingivalis. Journal of Periodontal Research 1999, 34(2), 70-78.
- Azman R, Lappin DF, MacPherson A, Riggio M, Robertson D, Hodge P, Ramage G, Culshaw S, Preshaw PM, Taylor J, Nile C. Clinical associations between IL-17 family cytokines and periodontitis and potential differential roles for IL-17A and IL-17E in periodontal immunity. Inflammation Research 2014, 63(12), 1001-1012.