Faculty of Medical Sciences

Staff Profile

Dr Paula Salgado

Lecturer in Macromolecular Crystallography

Background

Research Positions

2012 - present Lecturer of MacromolecularCrystallography, ICaMB, Newcastle University

2011 -2012 Research Associate, Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, UK

2008 – 2011 Research Associate, Division of Molecular Biosciences, Department of Life Sciences, Imperial College London, UK

2005 – 2008 Research Associate, School of Crystallography, Birkbeck College/University College, London, UK


Education

2001 – 2005 PhD in Structural Biology, Henry Wellcome Trust Building for Human Genetics, University of Oxford and Open University. "Structural Studies of RNA-dependent RNA polymerases"                         Supervisors: Dr Jon Grimes and Prof Dave I Stuart

1993 -1999 BSc in Biochemistry, Science Faculty & Institute of Biomedical Sciences “Abel Salazar”, University of Porto, Portugal. Research Project: "Structure determination of transthyretin variants"

 

Research Awards

October 2017 - October 2021 - Wellcome Trust Collaborative Award

November 2014 - November 2017 - New Investigator Research Grant, MRC

April 2013 - March 2014 - Royal Society Research Grant

March - June 2011 - Wellcome Trust Value In People Award

Oct 01 - Oct 2005 - PhD Fellowship (part of Human Frontier Science Program Research Grant)

 

Research

As structural biologist interested in proteins associated with pathogenicity and disease, I have recently established a Clostridium difficile Structural Microbiology group focusing on detailed structural and functional characterisation of proteins involved in key pathogenicity pathways in this major human pathogen. Combining these approaches, we are interested in C. difficile spores, the dormant, resistant cell forms that are the transmission vehicle, as well as surface proteins and their role in infection.

 

Clostridium difficile S-layer

 
C. difficile is now the most prevalent hospital acquired infection in the UK. These gram-positive bacteria have an outside para-crystalline layer (S-layer) that is presumed to act as a protective shield and that is thought to be implicated in virulence, 
host interaction and immune activation. We focus on understanding S-layer organisation, primarily by determining the structure of the major S-layer protein constituent, SlpA.

We are also studying other cell wall proteins identified in C. difficile, aiming to understand their macromolecular details with our structural studies that complement work developed at Sheffield and Glasgow.

 

Sporulation engulfment machinery 

Work in the Salgado lab, awarded an MRC New Investigator Grant (Nov 2014), has recently identified two essential proteins involved in the early stages of sporulation (Crawshaw et al., 2014, Serrano et al., 2016).

 

 

 

Publications