Newcastle Preclinical In Vivo Imaging Facility

In Vivo Imaging System

In Vivo Imaging System

This Wellcome Trust funded 3D optical imaging system allows non-invasive and real-time longitudinal monitoring of disease progression, cell trafficking and gene expression patterns. The system is capable of both luminescence and fluorescence modes of imaging.

Real-time in vivo imaging

The in vivo imaging system (IVIS) can image bioluminescence and fluorescence in up to 5 mice during a single scanning session. Images can be used to generate high quality 3D quantitative representations of bioluminescence and fluorescence (400–840 nm) in vivo and in real time.

The software enables easy switching between fluorescence and bioluminescence modes of imaging and can allow 2 spectral images to be integrated.

An added benefit of the system is that it incorporates a topographic laser scanner and can yield for single-view, diffuse tomographic reconstructions of internal sources.

IVIS techniques

  • Bioluminscent
  • Spectral un-mixing
  • Fluorescence, including epi-illumination and trans-illumination
  • 3D diffuse tomographic, including Fluorescent Imaging Tomography (FLIT) and Diffuse Luminescence Imaging Tomography (DLIT)

Potential applications of IVIS

  • Functional genomics
    • expression profiles and regulation study
    • protein–protein interaction
    • apoptosis study
  • Oncology
    • tumour growth and metastasis
    • tumour related gene study
  • Infectious disease
    • infectious pathway
    • molecular study
  • Stem cell research
    • tracking and functional analysis
  • Phamaceuticals
    • drug discovery (high throughput)
    • pharmacokinetics (PK)
    • absorption, distribution, metabolism and excretion (ADME)
  • Toxicology research
    • pharmaceutical toxicology
    • chemical toxicology
  • Gene delivery and therapy
    • expression kinetics and localisation

Animal monitoring

IVIS is equipped with an Isoflurane anaesthesia system providing accurate flows of 0.5 and 1.0 L/min of gas (O2).

IVIS stage temperature can be monitored where the subjects are placed.