BIO2031 : Methods in Biotechnology: Principles and Theory

Semester 2 Credit Value: 10
ECTS Credits: 5.0


To inform students about the technology that is currently used in the analysis and engineering of genes in this sector. Also to introduce the principles of allied technologies (proteomics, transcriptomics, and cell culture) and expose students to industry through a site visit and an introduction to intellectual property.

The module, along with others, provides students with the appropriate underpinning theoretical knowledge for the final year biotechnological modules and projects offered by the School.

Outline Of Syllabus

This module introduces the technology currently used in the analysis and engineering of genes. It also introduces the principles of allied technologies (proteomics, transcriptomics, and cell culture) and exposes students to industry through a site visit to a biotech company and provides and introduction to the use of model-guided design for experimentation in biotechnology

1. How to clone a bacterial gene.
2. How to isolate a cDNA encoding a specific protein.
3. DNA sequencing.
4. Protein engineering.
5. High-level regulated expression of heterologous genes in microbial systems (E. coli, P. pastoris).
6. Genome editing.
7. Creation of transgenic plants, animals and microorganisms; introduction to applications of the technology in medicine, agriculture and industry.
8. Outlines of proteomics, transcriptomics.
9. Outline of plant and animal cell culture.
10. Introduction to model-guided design for biotechnology.
11. Site visit to Centre for Product Innovation (CPI), Redcar.
12. Revision sessions/Exam surgeries.

Teaching Methods

Teaching Activities
Category Activity Number Length Student Hours Comment
Guided Independent StudyAssessment preparation and completion11:301:30Final exam
Guided Independent StudyAssessment preparation and completion180:309:00Revision for final exam
Scheduled Learning And Teaching ActivitiesLecture141:0014:00N/A
Scheduled Learning And Teaching ActivitiesLecture31:003:00Revision sessions / exam surgeries
Scheduled Learning And Teaching ActivitiesFieldwork15:005:00N/A
Guided Independent StudyReflective learning activity90:304:30Reflective blog
Guided Independent StudyIndependent study181:0018:00Lecture, field work and revision session follow up
Guided Independent StudyIndependent study301:3045:00Directed reading
Teaching Rationale And Relationship

The lectures provide factual material; the revision seminars/exam surgeries provide periods to discuss topics in greater detail; the field trip exposes the students to a biotechnological company, to their industrial site, and to explanations of their purposes and methods.

Assessment Methods

The format of resits will be determined by the Board of Examiners

Description Length Semester When Set Percentage Comment
Written Examination902A100N/A
Exam Pairings
Module Code Module Title Semester Comment
BIO2030Methods in Biotechnology: Principles, Theory and Practice2N/A
Assessment Rationale And Relationship

The exam is designed to test understanding, synthesis and analysis, and retention of the important concepts of recombinant DNA technology.

Reading Lists