Seahorse XF Analyser
The newest addition to the flow cytometry core facility, Agilent Seahorse XF technology measures cellular metabolism in live cells.
Living cells use one or both of the major energy producing pathways:
- mitochondrial respiration
Seahorse XF technology measures cellular metabolism in live cells in real time.
Seahorse XF TechnologySeahorse XF Technology
Solid-state sensors measure extracellular flux (oxygen consumption rate (OCR) and extracellular acidification rate (ECAR)) providing insight into mitochondrial function and glycolytic activity.
Seahorse XF sensors simultaneously measure both OCR and ECAR in every well of either 96 (Seahorse XF96 system) or 24 well plates (Seahorse XF24 system). All platforms support injection of up to 4 unique compounds per well. At FCCF we have two XF96 (one each at the medical school and Centre for Life) and one XF24 instrument at the Campus for Ageing and Vitality (CAV).