Newcastle University Protein and Proteome Analysis

Who we are

The Newcastle University Protein and Proteome Analysis (NUPPA) facility supports researchers with protein and proteome analysis both within and beyond the University. We collaborate with internal researchers as well as external academic and commercial partners, with a primary focus on discovery proteomics. Our workflows utilise both data-independent acquisition (DIA) and data-dependent acquisition (DDA) mass spectrometry methods, with the capability to develop more targeted assays such as multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) when required. Our instrumentation, including the Astral platform (acquired via MRC capital equipment grant), provides the potential to meet a wide range of analytical needs by delivering both high sensitivity and high throughput. The facility has extensive experience working with a diverse range of sample types, including cell lysates, immunoprecipitation (IP) pull-downs, bacterial lysates, gel bands, and clinical samples such as plasma, cerebrospinal fluid (CSF), and tissue.

Prices for external collaborators starts at £275 per sample; including sample processing, data acquisition and analysis.

O-Link Reveal accredited service provider

Olink Reveal enables researchers to explore biological pathways in human health and disease and identify biomarkers for disease progression and treatment monitoring. Because proteins are the key effectors of cellular processes, measuring their abundance in blood and other liquid biopsy samples provides direct insight into functional biology.

The platform uses high-plex, affinity-based proteomics to quantify ~1,000 carefully selected proteins in a single analysis, offering broad pathway coverage and deep immune profiling across a wide range of diseases and research areas.

Available Services