NUPPA is equipped with:
- four LCMS systems
- one MALDI-TOFTOF
- chromatographic and electrophoretic equipment for the separation and characterisation of proteins and peptides
- analytical ultracentrifuge
- differential scanning calorimeter
- isothermal scanning calorimeter
We also have a wide range of software to perform project-bespoke data analysis.
Thermo LTQ XL Orbitrap
The Thermo LTQ XL Orbitrap is a high resolution mass spectrometer. It is very well suited for the analysis of complex proteomic samples.
Bruker maXis 4G
The Bruker maXis 4G is an Ultra High Resolution QqTOF mass spectrometer. It is particularly useful for the high resolution analysis of intact proteins on a chromatographic timescale.
Bruker Ultraflex II MALDI-TOFTOF
The Bruker Ultraflex II MALDI-TOFTOF is a mass spectrometer. It performs very well in the analysis of peptide mass fingerprints.
Other applications include the analysis of small molecules (in collaboration with ARRO Nanoscience) and the rapid identification of microorganisms.
Bruker HCT Proteome Discovery System
The Bruker HCT Proteome Discovery System is a fast 3D trap mass spectrometer. It has an excellent implementation of Electron Transfer Dissociation.
It is used for the analysis of very large, multiply charged peptides or of peptides that carry labile post-tramslational modifications.
ABSciex QTrap 4000
The ABSciex QTrap 4000 is ideally suited for highly mutiplexed single ion monitoring experiments. This is the preferred technique for the targeted quantitation of small molecules (including peptides).
This type of assay can be used as a 'mass spectrometry ELISA'. It has several advantages.
The method development is generally faster than for immunochemistry based methods. Fewer expensive consumables are needed. More analytes can be analysed in a single assay (more analytes per sample).
The main disadvantage is that turnaround per sample is lower than for ELISAs. Fewer samples can be analysed per day.