Bio-Imaging Unit

Leica DM5500

Leica DM5500 wide field fluorescence (upright)

Overview

The DM5500 is a high quality semi-automated upright wide field microscope capable of either fluorescence, brightfield/ DIC or both, controlled using LAS-AF or LAS software (Leica).

Filters, condenser and z drive are automated, allowing multichannel, z stack imaging to be performed.

The system utilises two separate camera systems for colour and monochrome imaging.

A range of fluorescent filters are available which cover the commonly used dyes, DAPI, Alexa488, Alexa594 and Alexa647.

The illumination source is a wide spectrum alignment free metal halide lamp.

Analysis of data/images can be carried out using the LAS software which includes quantification and colocalisation modules.

Specification

Here you can find the specifications for this microscope.

Imaging modes

Wide field fluorecuorescence, brightfield, volume x,y,z (3D), DIC

Example filters

  • DAPI
  • GFP
  • Cy3
  • Texas Red
  • Cy5

Example objectives

  • 100x (oil, 1.4)
  • 63x (oil, 1.4)
  • 40x (oil, 1.3)
  • 40x (air, 0.75)
  • 20x (air, 0.7)
  • 10x (air, 0.3)
  • 5x (air, 0.15)

Location

This equipment is located in the Campus for Ageing and Vitality.

It is in the Ageing Research Labs room F.19.

Campus for Ageing and Vitality
Newcastle University
Westgate Rd
Newcastle upon Tyne
NE4 9AQ

Here, you can get directions to the building.

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

The minimum booking time for this system is 30 minutes. Time increments of 15 minutes are permitted.

Before booking this equipment, please ensure the following:

  • any users are fully trained, and approved by a member of BioImaging facility staff
  • microscopes are left in a good state of tidiness (lenses/stage should be cleaned of any oil)
  • filters and lenses must not be removed or switched between systems without authorisation from Bioimaging facility staff

If you work at Newcastle University, you can book this microscope online by using iLab.

If you're a commercial company or an academic at another institution, just contact us to discuss your requirements.