Overview

The DM5500 is a high quality semi-automated upright wide field microscope capable of either fluorescence, brightfield/ DIC or both.

It is equipped with a full range of objectives, monochrome and colour cameras and filtersets to enable fluorescence imaging of up to four distinct channels from UV to far red excitation. Filters, condenser and z drive are automated, allowing multichannel, z stack imaging to be performed.

The system utilises two separate camera systems for colour and monochrome imaging.

Imaging Modes:

• 2D (xy) or 3D (xyz) multichannel

Specification

Here you can find the specification for this microscope.

Objectives

Magnification	NA	Coverslips	Other Modes	Immersion
5	0.15			Air
10	0.4	0.17		Air
20	0.8	0.17		Air
40	0.85	0.17		Air
40	1.3	0.17		Oil
63	1.4	0.17		Oil
100	1.44	0.17	DIC	Oil

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Filters (epi)

Cube	Example Fluorophores	Excitation	Dichroic	Emission	Part Number
Leica DAPI	DAPI, Hoechst	340-380		450-490
Leica GFP		450-490		500-550
Leica Cy3		541-551		580-620
Leica Texas Red		540-580		592-668
Leica Cy5		590-650		662-738

Light sources

• Lumencor SOLA SE365 LED light engine for epifluorescence
• Halogen lamp for transmission

Cameras

• Leica DFC360FX (monochrome 1.3MP CCD)
• Leica DFC420C (colour 5MP CCD)

Location

Biogerontology Building, Room F19

Health Innovation Neighbourhood
Newcastle University
Westgate Rd
Newcastle upon Tyne
NE4 9AQ

Booking

Find out how to book this microscope.

Booking information

• The minimal booking time for this system is 30 minutes. 
• Time increments of 15 minutes are permitted. 
• Bookings can be cancelled with no charge up to an hour before scheduled use. 

SOP

Leica DM5500 filter choies