Bio-Imaging Unit

Nikon BioStation

Nikon BioStation

Overview

The Nikon BioStation CT allows the imaging of cells without ever removing them from the incubator, it is therefore an ideal platform to image for long periods ‘sensitive’ cells in culture.

It has the capacity to permit the running of multiple experiments at the same time. Briefly, the system is comprised of a typical tissue culture incubator with the essentials of a fluorescence microscope inside. It is capable of three-colour (violet, green and red) imaging as well as capturing phase-contrast images.

The BioStation CT can accommodate up to 30 vessels ranging from 96 well plates to 75cm2 flasks. All manipulations of the vessels within the Bio-Station CT are automated and are carried out by a robotic arm.

CO2 , humidity, and temperature can all be regulated within the incubator.

The BioStation CT is capable of imaging from 2x to 10x phase images with apodized phase contrast (APC) optics. Fluorescence images are obtained using three color LED illumination. A color macro view allows the entire vessel to be viewed from above. Multi point multi-well imaging is achieved through a precision-controlled motorized XY-stage. Autofocus control and motorized Z acquisition ensures that images are always captured in the same focal plane.

Sample security measures mean that users access only the samples for which they have clearance. Experimental results are reliably traced and can be accessed remotely via an internet connection or LAN.

Specification

Here you can find the specifications for this microscope.

Imaging modes

  • multi point x,y (2D) with tiling
  • volume x,y,z (3D)
  • flourescece
  • phase
  • time lapse x,y,z,t (4D)

Objectives

  • 2x (air)
  • 4x (air)
  • 10x (air)
  • 20x (air)
  • 40x (air)

Location

This microscope is located in the Central Unit, on campus.

The Central Unit is based in the Bioimaging Unit in the William Leech Building, room M2.142.

Newcastle University
William Leech Building
Medical School
Framlington Place 
Newcastle upon Tyne
NE2 4HH

Here, you can get directions to the building.

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

The minimal booking time for this equipment is 30 minutes. Time increments of 15 minutes are permitted.

Bookings can be cancelled with no charge up to an hour before scheduled use.

Before booking this equipment, please ensure the following:

  • any users are fully trained, and approved by a member of BioImaging facility staff
  • microscopes are left in a good state of tidiness (lenses/stage should be cleaned of any oil)
  • filters and lenses must not be removed or switched between systems without authorisation from Bioimaging facility staff

If you work at Newcastle University, you can book this microscope online by using iLab.

If you're a commercial company or an academic at another institution, just contact us to discuss your requirements.