Overview

The Nikon E800 upright microscope is a manual upright widefield microscope capable of either fluorescence or brightfield imaging. It uses a colour camera controlled by LAS software (Leica).

The Nikon E800 upright microscope is a manual upright widefield microscope capable of either fluorescence or brightfield imaging. It uses a colour camera controlled by LAS-X software (Leica). The system has a full range of objectives and is ideal for histochemical imaging. Fluorescence imaging is more limited, with filters available for common dyes such as DAPI, Alexa488 or dual Alexa488 and Alexa594.

Imaging modes

• Wide field fluorescence
• Brightfield

Specification

Here you can find the specification for this microscope.

 Objectives

Magnification	NA	Coverslips	Other modes	Immersion
4	0.13		PhL	Air
10	0.3	0.17	DIC	Air
20	0.5	0.17	DIC N2	Air
40	0.75	0.17	DIC M	Air
60	1.4	0.17	DIC H	Oil
100	1.4	0.17	DIC N2	Oil

 

 

 

 

 

 

 

 

 

Filters (epi)

Cube	Example fluorophores	Excitation	Dichroic	Emission	Part Number
Nikon DAPI	DAPI, Hoechst	335-385	395	420-470
Nikon GFP	GFP, FITC, AF488	450-490	510	515-565
Nikon Dual Red/Green

 

 

 

 

 

Light source

Broad spectrum fluorescent light

Detectors, Cameras

Leica DFC420 colour camera (5MP CCD)

Location

Biogerontology Building, Room F.19

Health Innovation Neighbourhood
Newcastle University
Westgate Rd
Newcastle upon Tyne
NE4 9AQ

Booking

Find out how to book this microscope.

Booking information

• The minimal booking time for this system is 30 minutes. 
• Time increments of 15 minutes are permitted. 
• Bookings can be cancelled with no charge up to an hour before scheduled use.