Bio-Imaging Unit

Nikon E800

Nikon E800 wide field (upright)

Overview

The Nikon E800 upright microscope is a manual upright widefield microscope capable of either fluorescence or brightfield imaging. It uses a colour camera controlled by LAS software (Leica).

The system is ideal for histochemical imaging.

Fluorescent filters are available which cover the commonly used dyes:

  • DAPI
  • Alexa488
  • Alexa488 and Alexa594 (dual colour emission filter)

The illumination source is a 100W Hg lamp.

Analysis of data/images can be carried out using the LAS software or ImageJ.

Specification

Here you can find the specifications for this microscope.

Imaging modes

Wide field fluorecuorescence or brightfield

Example filters

  • DAPI
  • GFP
  • FITC/Cy3

Example objectives

  • 100x (oil, 1.4)
  • 60x (oil, 1.4)
  • 40x (air, 0.75)
  • 20x (air, 0.5)
  • 10x (air, 0.3)
  • 4x (air, 0.13)

Location

This equipment is located in the Campus for Ageing and Vitality.

It is in the Ageing Research Labs room F.19.

Campus for Ageing and Vitality
Newcastle University
Westgate Rd
Newcastle upon Tyne
NE4 9AQ

Here, you can get directions to the building.

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

The minimum booking time for this system is 30 minutes. Time increments of 15 minutes are permitted.

Before booking this equipment, please ensure the following:

  • any users are fully trained, and approved by a member of BioImaging facility staff
  • microscopes are left in a good state of tidiness (lenses/stage should be cleaned of any oil)
  • filters and lenses must not be removed or switched between systems without authorisation from Bioimaging facility staff

If you work at Newcastle University, you can book this microscope online by using iLab.

If you're a commercial company or an academic at another institution, just contact us to discuss your requirements.