Nikon NiE
Nikon NiE
Modalities: upright, widefield
Overview
The Nikon NiE is a semi-automated microscope.
The Nikon NiE microscope can be used to view slides. The camera is an Andor Zyla 5.2.
The filters, condenser, motorized stage and z drive are all automated. This allows for multichannel, z stack imaging to be performed.
The system is run through Nikon’s versatile Elements software package. It also incorporates wizards to allow the automated capture and analysis of fluorescence images.
Imaging modes
- Widefield fluorescence
- Phase Contrast
- XYZ (3D)
Specification
Here you can find the specifications for this microscope.
Objectives
Magnification |
NA |
Coverslip |
Other Modes |
Immersion |
| 4 | 0.2 | Air | ||
| 10 | 0.45 | 0.17 | Air | |
| 20 | 0.7 | 0.17 | Air | |
| 40 | 0.95 | 0.17 | Air | |
| 40 | 1.3 | 0.17 | Oil | |
| 100 | 1.4 | 0.17 | Oil |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part Number |
| Nikon DAPI | DAPI, Hoechst | 340-380 | 400 | 435-485 | |
| Nikon FITC | GFP, FITC, AF488 | 465-495 | 505 | 515-555 | |
| Nikon TRITC | TRITC | 527-552 | 565 | 557-633 | |
| Nikon Cy3 | Cy3 | 515-555 | 565 | 570-610 | |
| Nikon Cy5 | Cy5, AF647 | 590-650 | 660 | 665-735 |
Light source (epi)
CoolLed pE300 (Broad spectrum LED fluorescent light)
Detectors (camera)
Andor Zyla 5.5-Monochrome (for fluorescent imaging)
Location
NMJ Facility, Cookson Building, Dental School – G32
Medical School
Framlington Place
Newcastle University
Newcastle upon Tyne
NE2 4HH
Enter your postcode or address to find directions
Booking
Find out how to book this microscope.
Booking information
- The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.