Overview

The VisiTech iSIM confocal is an array-scanning confocal microscope based around a fully motorized, inverted Nikon TiE body.

This microscope provide live cell incubation with a high speed super resolution imaging system capable of capturing super resolution images in real time up to 1000 frames per second.

The system has two Hamamatsu Orca Flash 4 sCMOS monochrome cameras for simultaneous two colour fluorescence imaging.

The system is run through Nikon’s versatile Elements software package and incorporates wizards to perform common imaging tasks.

 

Imaging modes

• Multi point x,y (2D) with tiling,
• Volume x,y,z (3D),
• Time lapse x,y,z,t (4D)
• Live cell
• Superresolution

 

 

Specification

Here you can find the specifications for this microscope.

Objectives

Magnification	NA	Coverslip	Other modes	Immersion
10	0.3	0.17	DIC L/N1	Air
20	0.75	0.17	DIC N2	Air
60	1.25	0.17	DIC N2	Water
100	1.49	0.17	DIC N2	Oil

 

 

 

 

 

 

Filters(epi)

Cube	Example fluorophores	Excitation	Dichroic	Emission	Part number
Nikon DAPI	DAPI, Hoechst	340-380	400	435-485
Nikon FIRC	GFP, FITC, AF488	465-496	505	515-555
Nikon TxRED	Texas Red, Af594	540-580	595	600-660

 

 

 

 

 

Light source (epi)

Intensilight (Broad spectrum fluorescent light)

Laser lines

405nm, 488nm, 561nm, 640nm

Detectors (cameras or PMTs)

DUAL Hamamatsu ORCA-Flash-4.0 V2 

Location

William Leech Building, Room M2.147a

Newcastle University
William Leech Building
Medical School
Framlington Place 
Newcastle upon Tyne
NE2 4HH

Booking

Find out how to book this microscope.

Booking information

• The minimal booking time for this system is 30 minutes. 
• Time increments of 15 minutes are permitted. 
• Bookings can be cancelled with no charge up to an hour before scheduled use.