Overview

The Zeiss AxioVert 2 offers two modes of observation, Brightfield transmitted light (phase & DIC) and Widefield fluorescence.

The Zeiss AxioVert 2 system utilises a monochrome imaging camera.

A range of fluorescent filters are available which cover the commonly used dyes:

• DAPI
• FITC
• TRITC and CY5

The illumination source is a HBO100 mercury arc lamp.

The microscope has a heated stage insert for live samples.

Imaging modes

• Wide field fluorescence
• Brightfield
• Phase contrast

Specification

Here you can find the specifications for this microscope.

Objective

Magnification	NA	Coverslip	Other modes	Immersion
5	0.15	0.17	Ph1	Air
10	0.32	0.17	Ph1	Air
20	0.4	0.17	Ph2	Air
40	0.6	0.17	Korr Ph2 ELWD	Air
63	0.75	0.17	Korr Ph2 ELWD	Air

 

 

 

 

 

 

 

 

Filters (epi)

Cube	Example fluorophores	Excitation	Dichroic	Emission	Part Number
Zeiss 02	DAPI, Hoechst	335-390	395	420 (LP)
Zeiss 47 HE	CFP	424-448	455	460-500
Zeiss 13	eGFP	460-480	495	505-530
Zeiss 46 HE	FITC, AF488	487-512	515	520-550
Zeiss 15	dTomato,  MT Orange	540-552	580	590-LP

 

 

 

 

 

 

 

 

 

Light source (epi)

HBO 100 (Broad spectrum fluorescent light)

Detectors (cameras)

Zeiss AxioCam HRc (Colour camera used both for fluorescence and brightfield HC)

Location

East Wing, International Centre for Life room G322

Newcastle University
International Centre for Life
Central Parkway
Newcastle upon Tyne
NE1 3BZ

Booking

Find out how to book this microscope.

Booking information

• The minimal booking time for this system is 30 minutes. 
• Time increments of 15 minutes are permitted. 
• Bookings can be cancelled with no charge up to an hour before scheduled use.