Dr Bill Chaudhry
- Email: email@example.com
- Telephone: +44 (0) 191 241 8681
- Address: Biomedicine East
Institute of Genetic Medicine
Newcastle upon Tyne
Congenital heart malformation and disease
I have dual training, both as a paediatric intensivist and in also in developmental molecular genetics. Over the last few years I have gradually moved from the hospital intensive care unit into the University laboratory to pursue biomedical research. I am interested in inherited cardiac diseases that are present at birth, and usually structural , but also in other diseases, such as cardiomyopathy that develop later in life. Both knockout (Cre-lox) mice and zebrafish are used in these studies and a variety of molecular, genomic and proteomic approaches are used in this work.
Over the last decade Deb Henderson and I have been investigating how the heart forms using knockout mice and zebrafish. We have particularly focussed on Vangl2, a gene in the non-canonical Wnt signalling or planar cell polarity (PCP) pathway. The Looptail mouse, a naturally occurring Vangl2 mutant, has taught us a great deal about how the outflow tract (the aorta and pulmonary artery) develops and we are now examining if the developmental origins of these great vessels might be linked with adult onset disease. I work closely with Bob Anderson and especially try to link developmental cardiac morphology with molecular genetic approaches. I have also been looking at the possibility of modelling cardiomyopathy in adult zebrafish and using zebrafish heart development to study basic fundamental aspects that are conserved between species, such as left-right patterning and the heart.
In addition, microscopy and computing are particular interests. We have extensively used 3D reconstruction software in our studies and have developed our own sheet light microscopy System and 3D printer within the group.
I am now starting a major project to apply our knowledge of developmental biology of the heart to human congenital heart disease. Initially I am investigating Hypoplastic Left Heart Syndrome (HLHS), a rare abnormality in which there is severe underdevelopment of the main heart chamber and artery that pump blood out to the body. In these studies I am working with paediatric cardiologists both in Newcastle and Paris and also with the Paediatric Cardiothoracic Surgical Team at Birmingham Children’s Hospital. The initial goal is to understand what are the specific developmental problems underlying HLHS and then to apply developmental experience to identify causative genes in families with HLHS.
Deborah Henderson BSc PhD, Professor of Cardiac Development
Robert Anderson BSc, MD, FRCPath, FESC, FECTS, Visiting Professor
Lorraine Eley, BHF Senior Research Associate
Rachel Richardson, BHF Senior Research Associate
Lindsay Murphy, BHF Research Assistant
Natasha Curley, PhD student
Ahlam Alqahtani, PhD Student
- Phillips HM, Hildreth V, Peat JD, Murdoch JN, Kobayashi K, Chaudhry B, Henderson DJ. Non-cell-autonomous roles for the planar cell polarity gene vangl2 in development of the coronary circulation. Circulation Research 2008, 102(5), 615-623.
- Phillips HM, Rhee HJ, Murdoch JN, Hildreth V, Peat JD, Anderson RH, Copp AJ, Chaudhry B, Henderson DJ. Disruption of planar cell polarity signaling results in congenital heart defects and cardiomyopathy attributable to early cardiomyocyte disorganization. Circulation Research 2007, 101(2), 137-145.
- Phillips HM, Murdoch JN, Chaudhry B, Copp AJ, Henderson DJ. Vangl2 acts via RhoA signaling to regulate polarized cell movements during development of the proximal outflow tract. Circulation Research 2005, 96(3), 292-299.
- Henderson DJ, Phillips HM, Chaudhry B. Vang-like 2 and noncanonical Wnt signaling in outflow tract development. Trends in Cardiovascular Medicine 2006, 16(2), 38-45.
- McKinney PA, Jones S, Parslow R, Davey N, Darowski M, Chaudhry B, Stack C, Parry G, Draper ES, PICANet Consent Study Grp. A feasibility study of signed consent for the collection of patient identifiable information for a national paediatric clinical audit database. British Medical Journal 2005, 330(7496), 877-879.
- Chaudhry B, Burns D, Henderson DJ. Cardiomyocyte aging: swimming against the clock. In: 2nd Congress of the European Society of Cardiology Council on Basic Cardiovascular Science: Frontiers in Cardiovascular Biology. 2012, London, UK: Oxford University Press.
- Bamforth SD, Chaudhry B, Bennett M, Wilson R, Mohun TJ, van Mierop LHS, Henderson DJ, Anderson RH. Clarification of the identity of the mammalian fifth pharyngeal arch artery. Clinical Anatomy 2013, 26(2), 173-182.
- Henderson DJ, Boczonadi V, Humbert P, Chaudhry B. Conditional deletion of Scrib gene in the developing myocardium leads to congenital heart defects. In: 2nd Congress of the European Society of Cardiology Council on Basic Cardiovascular Science: Frontiers in Cardiovascular Biology. 2012, London, UK: Oxford University Press.
- Hunt NC, Batsivari A, Chaudhry B, Henderson DJ. ECM control of neural crest cell (NCC) behaviour and implications for heart development. In: Autumn Meeting of the British Society for Matrix Biology (BSMB). 2012, Newcastle upon Tyne, UK: Wiley-Blackwell Publishing Ltd.
- Chaudhry B, Chrystal P, Henderson DJ. Fast Digital Laser Sheet Microscopy: swimaging in 6 dimensions. In: 2nd Congress of the European Society of Cardiology Council on Basic Cardiovascular Science: Frontiers in Cardiovascular Biology. 2012, London, UK: Oxford University Press.
- Phillips HM, Papoutsi T, Soenen H, Ybot-Gonzalez P, Henderson DJ, Chaudhry B. Neural Crest Cell Survival is Dependent on Rho Kinase and is Required for Development of the Mid Face in Mouse Embryos. PLoS One 2012, 7(5), e37685.
- Anderson RH, Chaudhry B, Mohun TJ, Bamforth SD, Hoyland D, Phillips HM, Webb S, Moorman AF, Brown NA, Henderson DJ. Normal and abnormal development of the intrapericardial arterial trunks in humans and mice. Cardiovascular Research 2012, 95(1), 108-115.
- Keenan ID, Rhee HJ, Chaudhry B, Henderson DJ. Origin of non-cardiac endothelial cells from an Isl1+ lineage. FEBS Letters 2012, 586(13), 1790-1794.
- Henderson DJ, Chaudhry B. Getting to the Heart of Planar Cell Polarity Signaling. Birth Defects Research Part A: Clinical and Molecular Teratology 2011, 91(6), 460-467.
- Simms RJ, Hynes AM, Eley L, Inglis D, Chaudhry B, Dawe HR, Sayer JA. Modelling a ciliopathy: ahi1 knockdown in model systems reveals an essential role in brain, retinal and renal development. Cellular and Molecular Life Sciences 2011, 69(6), 993-1009.
- Taylor JM, Saunter CD, Love GD, Girkin JM, Henderson DJ, Chaudhry B. Real-time optical gating for three-dimensional beating heart imaging. Journal of Biomedical Optics 2011, 16(11), 116021.
- Anderson RH, Cook A, Brown NA, Henderson DJ, Chaudhry B, Mohun T. Development of the outflow tracts with reference to aortopulmonary windows and aortoventricular tunnels. Cardiology in the Young 2010, 20(s3), 92-99.
- Hammond KL, Loynes HE, Mowbray C, Runke G, Hammerschmidt M, Mullins MC, Hildreth V, Chaudhry B, Whitfield TT. A Late Role for bmp2b in the Morphogenesis of Semicircular Canal Ducts in the Zebrafish Inner Ear. PLoS One 2009, 4(2), e4368.
- Bradshaw L, Chaudhry B, Hildreth V, Webb S, Henderson DJ. Dual role for neural crest cells during outflow tract septation in the neural crest-deficient mutant Splotch(2H). Journal of Anatomy 2009, 214(2), 245-257.
- Hildreth V, Webb S, Chaudhry B, Peat JD, Phillips HM, Brown N, Anderson RH, Henderson DJ. Left cardiac isomerism in the Sonic hedgehog null mouse. Journal of Anatomy 2009, 214(6), 894-904.
- Ioannides AS, Chaudhry B, Henderson DJ, Spitz L, Copp AJ. Dorsoventral patterning in oesophageal atresia with tracheo-oesophageal fistula: Evidence from a new mouse model. Journal of Pediatric Surgery 2002, 37(2), 185-191.
- Ng WF, Hernandez-Fuentes M, Baker R, Chaudhry A, Lechler RI. Reversibility with Interleukin-2 Suggests that T Cell Anergy Contributes to Donor-Specific Hyporesponsiveness in Renal Transplant Patients. Journal of the American Society of Nephrology 2002, 13(12), 2983-2989.
- Ng WF, Baker RJ, Hernandez-Fuentes M, Chaudhry A, Lechler RI. The role of T-cell anergy in the maintenance of donor-specific hyporesponsiveness in renal transplant recipients. Transplantation Proceedings 2001, 33(1-2), 154-155.
- Chaudhry B, Burns D, Murphy L, Henderson D. Exercise and cardiomyocyte turnover in the aging zebrafish heart. In: Third Congress of the ESC Council on Basic Cardiovascular Science: Frontiers in Cardiovascular Biology. 2014, Barcelona, Spain: Oxford University Press.
- Hill D, Verykiou S, Robinson N, Chaudhry B, Przyborski S, Lovat P. Modelling melanoma metastasis using organotypic skin equivalent and zebrafish models. In: British Society for Investigative Dermatology Annual Meeting. 2014, Newcastle, UK: Nature Publishing Group.
- Phillips HM, Mahendran P, Singh E, Anderson RH, Chaudhry B, Henderson DJ. Neural crest cells are required for correct positioning of the developing outflow cushions and pattern the arterial valve leaflets. Cardiovascular Research 2013, 99(3), 452-460.
- Anderson RH, Henderson DJ, Chaudhry B. Development and maintenance of the arterial valves. European Heart Journal 2017, 38(9), 687-689.
- Piatek MJ, Henderson V, Fearn A, Chaudhry B, Werner A. Ectopically expressed Slc34a2a sense-antisense transcripts cause a cerebellar phenotype in zebrafish embryos depending on RNA complementarity and Dicer. PLoS ONE 2017, 12(5), e0178219.
- Anderson RH, Bamforth SD, Spicer DE, Henderson DJ, Chaudhry B, Brown NA, Mohun TJ. Development and maldevelopment of the ventricular outflow tracts. In: Lacour-Gayet F; Bove EL; Hraška V; Morell V; Spray T, ed. Surgery of Conotruncal Anomalies. Cham, Switzerland: Springer International Publishing, 2016, pp.27-59.