Electron Microscopy Research Services

Serial Block Face Imaging

Serial Block Face Imaging

Create 3D reconstructions at the ultrastructural level.

Thanks to a successful BBSRC bid we can now offer serial block face imaging with the Zeiss Sigma Scanning Electron Microscope (FEGSEM) incorporating the Gatan 3view. (SBF-SEM)

Data from SBF-SEM can be organised into a stack of images. By segmenting out an area of interest, in each image in the stack, you can then generate a 3D model.

Specialist software can be used to display and edit the 3D representation of your data.

Software available to 3view users:

  • Amira  
  • Blender
  • MIB
  • IMOD
  • ImageJ  
  • Digital Micrograph

In EMRS we offer training on 3D reconstruction and biological image analysis and the related software.

3view reconstruction of neuromuscular junction folds