Our lab houses a Philips CM100 TEM with Compustage and high resolution digital image capture. This allows very accurate measuring, image collection, montage and random sampling.
Typical applications for TEM:
- plant, animal tissue and cell culture
- isolated proteins
- bacteria and viruses
- particulate size and distribution in non-biological samples
Experienced staff are here to provide support in all areas of TEM. Training is available for Newcastle University researchers which will reduce costs in the long term.
Standard epoxy resin embeddingStandard epoxy resin embedding
Ultrathin sections are stained with heavy metal salts (uranyl acetate and lead citrate) in order to increase the density of the samples and provide contrast to the image.
This technique is used for most resin-embedded biological samples.
Negative stainingNegative staining
A concentrated suspension of particles is deposited onto a coated grid. A drop of heavy metal salt is applied to the grid and surrounds the sample. The electron beam will pass through the sample easier than the surrounding stain and in the resulting image the sample particles will appear light and the surrounding area dark.
This technique is used for the visualisation of bacteria flagellae, viruses, and isolated proteins.
Research expertise in the area of protein analysis is provided by Prof. Robin Harris, who works closely with teams led by Prof. Rick Lewis, and Prof. Jeremy Lakey.
Pre-embedding immunogoldPre-embedding immunogold
Immuno-labelling with colloidal gold is a technique used for the localisation of antigenic sites at the ultrastructural level.
Pre-embedding enzyme histochemistryPre-embedding enzyme histochemistry
Enzyme histochemistry using 3,3-diaminobenzidine (DAB) can be visualised at the ultrastructural level as the DAB reacts with osmium tetroxide to give an electron dense deposit.
The procedure is carried out on tissue sections prior to embedding in resin.