Leica DMi8
Modalities: invert, widefield, live cell
Overview
A fully automated widefield system for fluorescence and brightfield imaging.
The DMi8 is a fully automated widefield system for fluorescence and brightfield imaging. It is equipped with a full range of objectives, monochrome and colour cameras and filter sets to enable fluorescence imaging of up to four distinct channels from UV to far red excitation.
Stage and z are also motorised, allowing multichannel z stacks in multiple positions or tile scans, plus in combination with an Okolab on-stage incubator, live cell imaging is also possible.
Imaging Modes
- 2D (xy) or 3D (xyz), multichannel with timelapse and/or tiling and/or multiple locations
Specification
Here you can find the specifications for this microscope.
Objectives
Magnification |
NA |
Coverslips |
Other Modes |
Immersion |
|---|---|---|---|---|
| 5 | 0.15 | Air | ||
| 10 | 0.4 | 0.17 | Air | |
| 20 | 0.8 | 0.17 | Air | |
| 40 | 0.85 | 0.17 | Air | |
| 40 | 1.3 | 0.17 | Oil | |
| 63 | 1.4 | 0.17 | Oil | |
| 100 | 1.44 | 0.17 | DIC | Oil |
Filters (epi)
Cube |
Example Fluorophores |
Excitation |
Dichroic |
Emission |
Part Number |
|---|---|---|---|---|---|
| Leica DAPI | DAPI, Hoechst | 325-375 | 400 | 435-485 | |
| Leica GFP | GFP, FITC,AF488 | 450-490 | 500-550 | ||
| Leica Rhod | Cy3, AF555 | 541-551 | 565-605 | ||
| Leica TXR* | AF568, AF594 | 540-580 | 585 | 592-668 | |
| Leica CY5 | CY5, AF647 | 560-650 | 662-738 | ||
| Leica mK-Red | mK-red | 550-570 | 602-682 | ||
| Leica mK-Green | mK-green | 420-460 | 602-682 |
*fitted on request
Light Sources
- Lumencor SOLA SE365 LED light engine for epifluorescence
- White LED for transmission
Cameras
- Hamamatsu Flash 4 v2, (monochrome 4MP sCMOS)
- Leica DFC450 (colour 5MP CCD)