Nikon FN1 (FLAME)
Overview
Nikon FN1 developed especially for electrophysiological research, features enhanced operability to facilitate experiments.
This equipment was funded by [BBSRC, 2020], please reference the funder and [BB/T017627/1], in your publication
The Nikon FN1 in the FLAME facility has been designed to allow maximum flexibility for different kind of imaging experiments coupled (or not) with the two other main components of the system: the BioCam Duplex MEA system and the Polygon 1000 patterned illuminator. Depending on the experimental design these components can be added, removed, or modified by the facility staff.
The system is run through Nikon’s versatile Elements-JOBS software package and incorporates wizards to allow the automated capture and analysis of both fluorescence and colour images.
Specification
Objectives
|
Magnification |
NA |
Coverslips |
Other modes |
Immersion |
|---|---|---|---|---|
| 2 | 0.1 | Air | ||
| 4 | 0.2 | Air | ||
| 10 | 0.3 | DIC | Water Dipping | |
| 16 | 0.8 | DIC | Water Dipping | |
| 20 | 0.45 | Air | ||
| 40 | 0.8 | DIC | Water Dipping | |
| 60 | 1 | DIC | Water Dipping |
Filters (epi)
| Cube | Example fluorophores | Excitation | Dichroic | Emission | Part Number |
|---|---|---|---|---|---|
| Chrome 89402 | DAPI/FITC/TRITC/Cy5 |
375-407 466-495 542-566 623-653 |
413 500 573 660 |
418-450 506-531 577-609 665-724 |
|
| Chroma 89403 | CFP/YFP/RFP/Cy7 |
422-450 496-518 565-589 |
458 524 598 |
462-486 528-552 708-752 |
|
| Chroma 49053 | DAPI | 350-420 | 425 | 440-480 | |
| Chroma 49001 | CFP | 426-446 | 455 | 460-500 | |
| Chroma 49002 | GFP/FITC/Cy2 | 450-490 | 495 | 500-550 | |
| Chroma 49003 | YFP | 490-510 | 515 | 520-550 | |
| Chroma 49004 | Cy3/TRITC/ | 532-557 | 565 | 570-640 | |
| Chroma 49008 | mCherry/Texas red | 540-580 | 585 | 593-668 | |
| Chroma 49009 | Cy5/AF647 | 625-655 | 660 | 665-715 | |
| Chroma 49007 |
Cy7/AF750 |
560-584 | 590 | 598-660 | |
| Chroma 79002 | Fura 2/BCECF (ratiometric) |
335-350 375-390 426-446 490-500 |
510 | 520-560 |
Light source
CoolLed pE800
CoolLed pE340
Detectors (cameras, PMTs)
Photometric Prime BSI express.