Overview

Zeiss LSM 880 upright confocal multiphoton microscope with Airyscan detector.

The LSM880 is a point scanning confocal microscope built on an inverted microscope body. There are a range of objectives, optimally chosen for in vivo or high resolution multiphoton microscopy. The microscope is all automated except for the condenser. The focus drive can be driven by the software, and a fast piezo drive is available (for z stacks up to 400 nm). Optionally, the system can be inverted to allow using the microscope as an upright imaging system using an LSMTech Inverterscope.

For confocal imaging, there are 6 continuous wave (CW) excitation laser lines and 2 pulsed lasers, one fixed at 1040 nm plus one variable (680-1300 nm) for Non Linear Optical (NLO)/ multiphoton microscopy.

For detection, a choice of 4 internal detectors: 2 photomultiplier tubes (PMTs) and 1 GaAsP detector in the scanhead using a shared pinhole, plus an Airyscan 32x GaAsP array. The Airyscan module can be used in several modes, enabling super-resolution, virtual pinhole or Airyscan fast modality. For multiphoton imaging, there is also a linear array of 4 external non descanned detectors (NDDs), 2 PMTs and 2 GaAsPs with a choice of filters in reflective position.

The system is equipped with a blacked out heated incubator and CO2 module to enable stable operating temperature for in vivo imaging.

Imaging Modes:

• Confocal, Airyscan or Airyscan Fast fluorescence
• 2 photon and 3 photon fluorescence
• Second Harmonic Generation

All modalities can be performed in 2D (xy) or 3D (xyz), multichannel with timelapse and/or tiling and/or multiple locations, plus ROI scanning allowing FRAP and FRET.

 

Specification

Here you find the specification for this microscope.

Objectives

Magnification	NA	Coverslip	Other modes	Immersion
10	0.45	0.17		Water
25	0.8	0.17		Multi immersion, water/ oil/ silicone or glycerine with or without coverslip
32	0.8	0.17		Water
100	1.45			Oil
40	1.3	0.17		Oil
63	1.4			Oil
40	1.3			Oil
20	0.7			Air
10	0.45			Air

 

 

 

 

 

 

 

 

 

Filters (epi)

For the three single internal detectors (2x PMT, 1x GaAsP), freely moveable bandpass or longpass filters from 380-760nm.

For the internal Airyscan detector the following dual filtersets are available:

• BP 420-480 + BP 495-550
• BP 420-480 + BP 495-620
• BP 465-505 + LP 525
• BP 495-535 + LP 555
• BP 495-550 + LP 570
• BP 555-620 + LP 645

For the NDDs, the following filtercubes are available:

EM. filter 1	Dichroic	EM. filter 2
BP 380-430	LP 445	empty
SP 485	LP 490	empty
BP 500-530	LP 550	BP 570-610
BP 500-550	LP 555	empty
BP 570-610	LP 625	empty
BP 645-710	Mirror	empty
BP 465-515	Mirror	empty
EF 485	LP 490	empty
BP 380-430	LP 445	empty
BP 453-467	LP 470	empty
BP 517.5-525.5	LP 532	BP 580-620

Other filtersets for the NDDs can be built upon request if none match your needs.

Lasers lines

CW lasers

• Argon, providing 458, 488 and 514nm
• HeNe 543 nm
• HeNe 633 nm
• 405 nm

Pulsed Lasers

• 1040 nm, 80 MHz, 100 fs.
• 680-1300 nm, 80 MHz, ~100fs

Detectors

• Internal 2 x PMT, 1 x GaAsP and 1 x Airyscan 32-GaAsP array
• External, 2 x PMT and 2 x GaAsP NDDs

Location

BioImaging Unit, Room M2.146, William Leech Building, Medical School 

Medical School
Newcastle University
Newcastle upon Tyne
NE2 4HH

Booking

Find out how to book this microscope.

Booking information

• The minimal booking time for this system is 30 minutes. 
• Time increments of 15 minutes are permitted. 
• Bookings can be cancelled with no charge up to an hour before scheduled use.