Leica DM6
Leica DM6
Modalities: upright, widefield, stereology
Overview
This microscope is a fully automated widefield system for fluorescence and brightfield imaging.
The DM6 has a full range of objectives (10- 100x), monochrome and color cameras and filter sets to enable fluorescence imaging of up to 5 distinct channels from UV to far red excitation.
Stage and z are also motorized, allowing multichannel z stacks in multiple positions or tile-scans.
The system can be operated using one of two software packages; Leica LASX or MBF StereoInvestigator.
Imaging modes
-
Widefield Fluorescence
- Multipoint x,y with tiling (2D)
- Volume x,y,z (3D)
- DIC
- Phase Contrast
- Stereology
- Time-lapse x,y,z,t (4D)
Specification
Here you can find the specification for this microscope.
Objectives
Magnification |
NA |
Coverslips |
Other Modes |
Immersion |
| 2.5 | 0.07 | Air | ||
| 5 | 0.15 | Air | ||
| 10 | 0.4 | 0.17 | Air | |
| 20 | 0.7 | 0.17 | Ph 2 DIC | Air |
| 40 | 0.85 | 0.17 | Air | |
| 63 | 1.32 | 0.17 | Ph3 CS | Oil |
| 100 | 1.44 | 0.17 | DIC | Oil |
Filters (epi)
Cube |
Example Fluorophores |
Excitation |
Dichroic |
Emission |
Part Number |
| Leica DAPI | DAPI, Hoechst | 325-375 | 400 | 435-485 | |
| Leica L5 | GFP, FITC,AF488 | 460-500 | 505 | 512-542 | |
| Leica N3 | AF555 | 540-552 | 565 | 580-620 | |
| Leica TXR | AF568, AF594 | 540-580 | 585 | 593-667 | |
| Leica Y5 | AF647 | 608-648 | 669 | 672-712 |
Light source (epi)
Sola Light SE - Broad spectrum fluorescent light
Cameras
Hamamatsu Flash 4, Monochrome (for fluorescence imaging)
Leica DFC450C, Colour (for brightfield HC)
Location
Paul O'Gorman Building, Room 2.004A
Paul O'Gorman Building
Newcastle University
Newcastle upon Tyne
NE2 4AH
Enter your postcode or address to find directions
Booking
Find out how to book this microscope.
Booking information
- The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.