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Bioimaging Unit
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Leica DM6

Modalities: upright, widefield, stereology

Overview

This microscope is a fully automated widefield system for fluorescence and brightfield imaging.

 The DM6 has a full range of objectives (10- 100x), monochrome and color cameras and filter sets to enable fluorescence imaging of up to 5 distinct channels from UV to far red excitation.  

Stage and z are also motorized, allowing multichannel z stacks in multiple positions or tile-scans.

The system can be operated using one of two software package; Leica LASX or MBF StereoInvestigator.

Imaging modes

  • Widefield Fluorescence 
  • Multipoint x,y with tiling (2D) 
  • Volume x,y,z (3D) 
  • DIC 
  • Phase Contrast 
  • Stereology 
  • Time-lapse x,y,z,t (4D) 

Specification

Here you can find the specification for this microscope.

Objectives

Magnification

NA

Coverslips

Other Modes

Immersion

2.5 0.07      Air
5 0.15      Air
10  0.4  0.17    Air
20  0.7  0.17  Ph 2 DIC  Air
40  0.85  0.17    Air
63  1.32  0.17  Ph3 CS  Oil
100  1.44  0.17  DIC  Oil

Filters (epi)

Cube

Example Fluorophores

Excitation

Dichroic

Emission

Part Number

Leica DAPI DAPI, Hoechst 325-375 400 435-485  
Leica L5 GFP, FITC,AF488 460-500 505 512-542  
Leica N3 AF555 540-552 565 580-620  
Leica TXR AF568, AF594 540-580 585 593-667  
Leica Y5 AF647 608-648 669 672-712  

Light source (epi)

Sola Light SE - Broad spectrum fluorescent light

Cameras

Hamamatsu Flash 4, Monochrome (for fluorescence imaging)

Leica DFC450C, Colour (for brightfield HC)

 

 

Location

Paul O'Gorman Building, Room 2.004A

Paul O'Gorman Building
Newcastle University
Newcastle upon Tyne
NE2 4AH

Booking

  • The minimal booking time for this system is 30 minutes
  • Time increments of 15 minutes are permitted
  • Bookings can be cancelled with no charge up to an hour before scheduled use
Make a booking

Training

This tutorial video shows you how to operate the microscope including start-up, software introduction, image capture, cleaning procedure and shutdown.