Skip to main content
Bioimaging Unit
Search for something
Search...

Leica M205

Modalities: upright, stereo, fluorescence

Overview

The M205 is a fluorescence capable stereo microscope with automated zoom and filter wheel. It can be equipped with 0.5, 1 or 2x objectives, allowing a zoom range from ~ 3x to 360x with focus stacking options post acquisition.

It is equipped with several illumination options:

  • base light for transmission
  • ringlight (on 1x objective)
  • fixed angle spotlights
  • Metal Halide lamp for fluorescence with a choice of filters for GFP, YFP or RFP

Image capture is via a colour camera using Las-X software.

Imaging Modes

  • Brightfield
  • Fluorescence
  • Both with optional focus stacking

Within our facility we have two M205 systems.

M205 (1) - Located in the the main facility will not do transmission.

M205 (2) - Located in the Cookson Building 4th floor is capable of fluorescence and transmission.

Specification

Objectives

Magnification

NA

Coverslips

Other Modes

Immersion

0.32  N/A      Air
1  N/A      Air
2  N/A      Air

 Filters (epi)

Cube

Example Fluorophores

Excitation

Dichroic

Emission

Part Number

Leica GFP2 GFP Pluc 460-500   5510 (LP)  
Leica YFP YFP 500-520   540-580  
Leica TXR Texas Red 540-580   610 (LP)  

Light Sources

  • Transmission lamp with adjustable angle
  • Ringlight with optional sidelighting
  • Fixed angle spotlights
  • Gooseneck (available upon request)
  • Metal Halide lamp for Fluorescence

Camera

  • DFC310FX (1.3MP CCD camera)

Location

Mitochondrial research group, 4th Floor Cookson building

Location for M205 (2)

Mitochondrial Reserach Group

4th Floor Cookkson Building
The Medical School
Framlington Place
Newcastle Upon Tyne
NE2 4HH
UK

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use. 
Make a booking