Leica SPE
Leica SPE
Modalities: invert, confocal, live cell
Overview
The Leica SPE is a point scanning confocal microscope.
The microscope is based around a fully motorized, inverted DMi8 body with hardware-enabled focus correction.
The system has one PMT detector for fluorescence imaging and one for transmitted light detection.
The system can also be operated in widefield fluorescence mode and perform regular 2D, 3D and 4D imaging with the ability for multi-point re-visiting and tiling.
The microscope is enclosed in an environmental chamber that can be heated and gas-balanced.
All image acquisition is controlled via LasX software.
Imaging modes
-
Multipoint x,y with tiling (2D)
- Volume x,y,z (3D)
- Fluorescence
- FRET
- FRAP
- DIC
- Time-lapse x,y,z,t (4D)
Specification
Here you can find the specification for this microscope.
Objectives
Magnification |
NA |
Coverslips |
Other modes |
Immersion |
| 10 | 0.3 | 0.17 | DIC | Air |
| 20 | 0.7 | 0.17 | Air | |
| 40 | 1.15 | 0.17 | DIC | Air |
| 63 | 1.3 | 0.17 | DIC | Oil |
Filters (epi)
Cube |
Example Fluorophores |
Excitation |
Dichroic |
Emission |
Part Number |
| Leica DAPI | DAPI, Hoechst | 340-380 | 400 | 420 (LP) | |
| Leica FITC | GFP, FITC, AF488 | 450-490 | 510 | 515 (LP) | |
| Leica RHOD | mCherry, AF594 | 515-560 | 580 | 590 (LP) |
Light source (epi)
Broad spectrum fluorescent light
Light source (Laser lines)
405nm,488nm,561nm,638nm
Detectors and Cameras
1 x PMT
Location
Paul O'Gorman Building, Room 2.004B
Paul O'Gorman Building
Newcastle University
Newcastle upon Tyne
NE2 4AH
Enter your postcode or address to find directions
Booking
Find out how to book this microscope.
Booking information
-
The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.