Bio-Imaging Unit

Nikon A1

Nikon A1 Confocal (Upright)


The Nikon A1+ is a point scanning confocal microscope based around a fully motorized, upright Nikon Ni body.

The system has four photomultipliers (two of which are GaAsP) for fluorescence imaging and an additional one for transmitted light detection.

Imaging can be performed at up to 4096 x 4096 pixel resolution.

The system is run through Nikon’s versatile Elements software package and incorporates wizards to perform common imaging tasks such as:

  • FRET
  • colocalisation
  • data quantification


Here you can find the specifications for this microscope.

Example objectives

LensImmersionNA (mm)
100x Oil 1.4
60x Oil 1.4
40x Oil 1.3
40x Air 0.6
20x Air 0.75
10x Air 0.4
4x Air 0.2

Imaging modes

  • multi point x,y (2D) with tiling
  • volume x,y,z (3D)
  • time lapse x,y,z,t (4D)

Laser lines

  • 405nm
  • 488nm
  • 561nm
  • 647nm


This microscope is located in the Central Unit, on campus.

The Central Unit is based in the William Leech Building room M2.154.

Newcastle University
William Leech Building
Medical School
Framlington Place 
Newcastle upon Tyne

Here, you can get directions to the building.

Enter your postcode or address to find directions


Find out how to book this microscope.

The minimal booking time for this equipment is 30 minutes. Time increments of 15 minutes are permitted.

Bookings can be cancelled with no charge up to an hour before scheduled use.

Before booking this equipment, please ensure the following:

  • any users are fully trained, and approved by a member of BioImaging facility staff
  • microscopes are left in a good state of tidiness (lenses/stage should be cleaned of any oil)
  • filters and lenses must not be removed or switched between systems without authorisation from Bioimaging facility staff

If you work at Newcastle University, you can book this microscope online by using iLab.

If you're a commercial company or an academic at another institution, just contact us to discuss your requirements.