Overview

The Nikon A1R is a point scanning confocal microscope based around a fully motorized, inverted Nikon Ti body with hardware-enabled focus correction.

The Nikon A1R has three photomultipliers for fluorescence imaging, one for transmitted light detection and a dedicated photomultiplier array for spectral profiling or if required, unmixing of fluorescently ‘complex’ specimens.

Incorporated into the confocal scan-head are both resonant (fast scanning) and non-resonant (conventional scanning) scanning mirrors. This allows experimenters to have the option of imaging in high resolution (up to 4096x4096 pixels), at high speed (420fps at 512x32 pixels) or a combination of both.

This arrangement is ideal for live-cell photo-activation and photo-bleaching studies where resonant scanning can be used to capture images at high-speed and non-resonant scanning can be used to target a region of interest for photo-activation.

Resonant scanning mode has added benefits; for live-cell experiments, photo-sensitive specimens remain live for longer and fluorophore bleaching is significantly reduced. The system will also perform regular 2D, 3D and 4D imaging.

The microscope is fully-enclosed in a heated environmental chamber and has the ability to set, maintain and monitor temperature, humidity, CO2 and O2 levels as imaging conditions dictate.

The system is run through Nikon’s versatile Elements software package and incorporates wizards to perform common imaging tasks such as FRAP, FRET and colocalisation as well as data quantification.

Specification

Here you can find the specifications for this microscope.

Objectives

Magnification	NA	Coverslips	Other modes	Immersion
4	0.2			Air
10	0.3	0.17		Air
20	0.75	0.17		Air
40	1.3	0.17		Oil
60	1.4	0.17		Oil
100	1.4	0.17		Oil

 

 

 

 

 

 

 

 

 

Filters (epi)

Cube	Example fluorophores	Excitation	Dichroic	Emission	Part Number
Nikon DAPI	DAPI, Hoechst	340-380	400	435-485
Nikon FITC	GFP, FITC, AF488	465-496	505	515-555
Nikon G2A	mCherry, AF594	510-560	565	590 (LP)

 

 

 

 

 

Light source

Intensilight (Broad spectrum fluorescent light) 

Laser lines

 

Location

West Wing, International Centre for Life, room B161

Newcastle University
International Centre for Life
Central Parkway
Newcastle upon Tyne
NE1 3BZ

Booking

Find out how to book this microscope.

Booking information

• The minimal booking time for this system is 30 minutes. 
• Time increments of 15 minutes are permitted. 
• Bookings can be cancelled with no charge up to an hour before scheduled use. 

Training video

This tutorial video shows you how to operate the microscope including start-up, image capture, cleaning procedure and shutdown.

SOP

Nikon A1R SOP