Overview

The microscope (Nikon TiE) is a fully automated and employs hardware and software focal drift correction. 

The system is equipped with both a mono (Andor Zyla 5.5 Mono) as well as with a color camera (Nikon DS-Fi1) which has been configured for multi-well plate-based screening applications but can be used to view slides with the appropriate stage insert. This system is equipped with an enviromental chamber for Live cell imaging with temperature and CO2 control. 

The system is run through Nikon’s versatile Elements-JOBS software package and incorporates wizards to allow the automated capture and analysis of both fluorescence and colour images. 

Imaging modes

• Multi point x,y (2D) with tiling
• Volume x,y,z (3D)
• Time lapse x,y,z,t (4D)
• Fluorescence
• DIC
• Brightfield imaging
• Live Cell imaging

Specification

Here you can find the specifications for this microscope.

Objectives

Magnification	NA	Coverslip	Other modes	Immersion
4	0.2			Air
10	0.45	0.17	DIC	Air
20	0.75	0.17		Air
40	0.6	0.17	DIC-Ph2	Air
40	0.95	0.17		Air

 

 

 

 

 

 

 

 

Filters (epi)

Cube	Example fluorophores	Excitation	Dichroic	Emission	Part Number
Nikon DAPI	DAPI, Hoechst	340-380	400	435-485
Chroma 49002-ET	GFP, FITC, AF488	450-490	495	500-550
Chroma 49003-ET	EYFP	490-510	515	520-550
Nikon G2A	mCherry, AF594	510-560	565	590 (LP)
Chroma 49006-ET	Cy5, AF647	590-650	650	665-735

 

Light source (epi)

CoolLed pE 340fura (340nm, 380nm)

Sola SE (Broad spectrum LED light)

Detectors (PMTs and cameras)

Andor Zyla 4.2 sCMOS

Nikon DS-Fi1

 

Location

BioImaging Unit, Room M2.154, William Leech Building, Medical School

 

Medical School
Newcastle University
Newcastle upon Tyne
NE2 4HH

Booking

Find out how to book this microscope.

Booking information

• The minimal booking time for this system is 30 minutes. 
• Time increments of 15 minutes are permitted. 
• Bookings can be cancelled with no charge up to an hour before scheduled use.