Overview

The Nikon A1R is a point scanning confocal microscope based around a fully motorized, inverted Nikon Ti body with hardware-enabled focus correction.

The Nikon A1R has three photomultipliers for fluorescence imaging, one for transmitted light detection and a dedicated photomultiplier array for spectral profiling or if required, unmixing of fluorescently ‘complex’ specimens.

Incorporated into the confocal scan-head are both resonant (fast scanning) (HD 1024x1024) and non-resonant (conventional scanning) scanning mirrors. This allows experimenters to have the option of imaging in high resolution (up to 4096x4096 pixels), at high speed (420fps at 512x32 pixels) or a combination of both.

This arrangement is ideal for live-cell photo-activation and photo-bleaching studies where resonant scanning can be used to capture images at high-speed and non-resonant scanning can be used to target a region of interest for photo-activation.

Resonant scanning mode has added benefits; for live-cell experiments, photo-sensitive specimens remain live for longer and fluorophore bleaching is significantly reduced. The system will also perform regular 2D, 3D and 4D imaging.

The microscope is fully-enclosed in a heated environmental chamber and has the ability to set, maintain and monitor temperature, humidity, CO2 and O2 levels as imaging conditions dictate.

The system is run through Nikon’s versatile Elements software package and incorporates wizards to perform common imaging tasks such as FRAP, FRET and colocalisation as well as data quantification.

Imaging modes

• Multi point x,y (2D) with tiling
• Volume x,y,z (3D)
• Time lapse x,y,z,t (4D)
• FRET
• FRAP
• Spectral
• Live Cell

Specification

Here you can find the specifications for this microscope.

Objectives

Magnification	NA	Coverslip	Other modes	Immersion
4	0.2			Air
10	0.45	0.17	DIC	Air
20	0.75	0.17	DIC	Air
40	0.95	0.17	DIC	Air
40	1.3	0.17		Oil

 

 

 

 

 

 

 

 

 Filters (epi)

Cube	Example fluorophores	Exctitation	Dicroich	Emission	Part Number
Nikon DAPI	DAPI, Hoechst	340-380	400	435-485
Nikon FITC	GFP, FITC, AF488	465-496	505	515-555
Nikon G2A	mCherry, AF594	510-560	565	590 (LP)

Light source (epi)

Intenslight (Broad spectrum fluorescent light)

Laser lines

405nm, 488nm, 561nm, 647nm

Detectors (cameras, PMTs)

2 x PMT, 2 x GaAsP,  1 x PMT for transmission 

 

Location

Bio-Imaging Unit, Room M2.142, William Leech Building, Medical School 

Newcastle University
William Leech Building
Medical School
Framlington Place 
Newcastle upon Tyne
NE2 4HH

Booking

Find out how to book this microscope.

Booking information 

Overview

The Nikon A1+ is a point scanning confocal microscope based around a fully motorized, upright Nikon Ni body.

The system has four photomultipliers (two of which are GaAsP) for fluorescence imaging and an additional one for transmitted light detection.

Imaging can be performed at up to 4096 x 4096 pixel resolution.

The system is run through Nikon’s versatile Elements software package and incorporates wizards to perform common imaging tasks such as:

• FRET
• colocalisation
• data quantification

SOP

Nikon A1R SOP