VisiTech iSIM
VisiTech iSIM
Modalities: invert, confocal, live cell
Overview
The VisiTech iSIM confocal is an array-scanning confocal microscope based around a fully motorized, inverted Nikon TiE body.
This microscope provide live cell incubation with a high speed super resolution imaging system capable of capturing super resolution images in real time up to 1000 frames per second.
The system has two Hamamatsu Orca Flash 4 sCMOS monochrome cameras for simultaneous two colour fluorescence imaging.
The system is run through Nikon’s versatile Elements software package and incorporates wizards to perform common imaging tasks.
Imaging modes
- Multi point x,y (2D) with tiling,
- Volume x,y,z (3D),
- Time lapse x,y,z,t (4D)
- Live cell
- Superresolution
Specification
Here you can find the specifications for this microscope.
Objectives
Magnification |
NA |
Coverslip |
Other modes |
Immersion |
10 | 0.3 | 0.17 | DIC L/N1 | Air |
20 | 0.75 | 0.17 | DIC N2 | Air |
60 | 1.25 | 0.17 | DIC N2 | Water |
100 | 1.49 | 0.17 | DIC N2 | Oil |
Filters(epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part number |
Nikon DAPI | DAPI, Hoechst | 340-380 | 400 | 435-485 | |
Nikon FIRC | GFP, FITC, AF488 | 465-496 | 505 | 515-555 | |
Nikon TxRED | Texas Red, Af594 | 540-580 | 595 | 600-660 |
Light source (epi)
Intensilight (Broad spectrum fluorescent light)
Laser lines
405nm, 488nm, 561nm, 640nm
Detectors (cameras or PMTs)
DUAL Hamamatsu ORCA-Flash-4.0 V2
Location
William Leech Building, Room M2.147a
Newcastle University
William Leech Building
Medical School
Framlington Place
Newcastle upon Tyne
NE2 4HH
Enter your postcode or address to find directions
Booking
Find out how to book this microscope.
Booking information
- The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.