Bioimaging Unit

Zeiss CellDiscoverer7

Zeiss CellDiscoverer7

Modalities: widefield, live cell, high content, long time-lapse

Overview

Automated Microscope for live cell imaging.

Our flagship high content system running Zeiss’ Zen software suite is a fully automated widefield microscope system complete with 40 place room temperature and 40 place incubated plate storage system with integrated robotic loader and scheduling system. Using a combination of air and water immersion lenses and a multi wavelength LED illumination system the CD7 allows for automated multiplate and multi slide imaging captured on a highly sensitive Hamamatsu Fusion sCMOS camera. The system also allows for a conditional or guided acquisition experiment to be designed and automatically analysed, further increasing the automation possibilities for large scale plate or slide arrays.

CD 7

Specification

Objectives

Magnification

NA

Coverslip

Other modes

Immersion

5 0.35 0.17   Air
20 0.7 0.17   Air
 50 1.2 0.17   Air

 

 

 

 

 

 

 

Filters (epi)

Cube

Example fluorophores

Excitation

Dichroic

Emission

Part number

Zeiss 49 DAPI, Hoechst 335-385  395 420-470   

Zeiss 38 HE

GFP, FITC, AF488 450-490 495 500-550  

Zeiss 91 HE LED

AF594/AF532/CFP/mCherry   450, 538, 610 457-477, 545-566, 617-765  

Zeiss 90 HE LED

DAPI/FITC/AF458/AF647   450, 493, 575, 653 410-440, 499-529, 580-604, 659-759  

Zeiss 568

AF568/mCherry/TRITC     574-631  

Zeiss 647

AF647     765-885  

 

Light Source (Epi)

Colibri 7 LED (358/30; 423/44; 469/38; 511/44; 567/30; 590/27; 625/33

Camera 

Monochrome CCD Zeiss 506

Monochrome sCMOS Hamamatsu Fusion

Location

Leech Building, M2.154

Medical School
Newcastle University
Newcastle upon Tyne
NE2 4HH

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Booking

Find out how to book this microscope.

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use. 

Training video

This tutorial video shows you how to operate the microscope including start-up, image capture, cleaning procedure and shutdown.