Overview

The LSM700 is a point scanning confocal microscope built on an inverted microscope body.

There are a full range of objectives and the microscope is automated except for the condenser arm and stage. The focus drive can be driven by the software, making z stacks possible.

For confocal imaging, there are 3 excitation laser lines (405, 488 and 561 nm) and for detection, 2 photomultiplier tubes (PMTs) in the scanhead using a shared pinhole which can be separated by a sliding dichroic, plus a transmission PMT for brightfield.

2 or 3 colour fluorescence imaging is therefore easy, and 4 or 5 colour imaging too, provided that good separation of the fluorophores is possible.

Finally, for short term live cell imaging, a stage top incubator is available, with temperature and CO2 regulation.

Imaging modes:

• Spot scanning, line scanning, 2D (xy) or 3D (xyz)
• multichannel with timelapse,
• ROI scanning with bleaching, allowing FRAP

Specification

Here you find the specification for this microscope.

Objectives

Magnification	NA	Coverslip	Other modes	Immersion
10	0.3	0.17		Air
20	0.5	0.17		Air
40	1.3	0.17	DIC	Oil
63	1.4	0.17	DIC	Oil immersion

 

 

 

 

 

 

 

 

Filters (epi) 

Cube	Example fluorophores	Excitation	Dichroic	Emission	Part number
Zeiss 10	AF488, FITC	450-490	510	515-565
Zeiss 20	TRITC, Rhodamine	540-552	560	575-640
Zeiss 49	DAPI, Hoechst	335-385	395	420-470

 

 

 

 

 

 

 

Sliding dichroic combined with SP555, LP560

Laser lines

Diode 405 nm, Solid state 488 and 561nm

Detectors

2 PMT plus 1 Transmission PMT

Location

Biogerontology Building, Room F1.48

Health Innovation Neighbourhood
Nuns Moor Road
Newcastle University
NE4 5PL

Booking

Find out how to book this microscope.

Booking information

• The minimal booking time for this system is 30 minutes. 
• Time increments of 15 minutes are permitted. 
• Bookings can be cancelled with no charge up to an hour before scheduled use. 

SOP

Zeiss LSM 700 SOP