Zeiss LSM880 Upright (NMJ)
Zeiss LSM880 upright
Modalities: upright, confocal, electrophysiology, airyscan, UV
Overview
The LSM880 with Electrophysiology set-up is a point scanning confocal microscope.
This equipment was funded by [Wellcome Trust, 2017], please reference the funder and [208339/Z/17/Z], in your publication.’
This system is built on an upright microscope body-Examiner Z1. It is a semi-automated microscope with a stationary fixed stage.
The objectives are specifically selected for electrophysiology therefore the majority of them are water immersion objectives.
The microscope is set-up for electrophysiology with the following set-up:
- Faraday cage
- 2 micromanipulators
- Axoclamp 900A
- Digidata 1550B
- Temp. Control
- Humbug
Imaging modes
- Multipoint x,y with tiling (2D)
- Volume x,y,z (3D)
- Fluorescence
- DIC
- Time-lapse x,y,z,t (4D)
- Z-stack and multi-color imaging of cell cultures, living sections, organs.
- Imaging + electrophysiology combining mode
- SuperResolution (AiryScan)
Specification
Here you can find the specification for this microscope.
Objectives
Magnification |
NA |
Coverslip |
Other modes |
Immersion |
Currently in use: |
||||
| 5 | 0.16 | Air | ||
| 10 | 0.45 | 0.17 | DIC | Water Dipping |
| 40 | 1 | 0.17 | DIC | Water Dipping |
| 63 | 0.9 | 0.17 | DIC | Water Dipping |
Additional available lenses: |
||||
| 2.5 | 0.12 | Air | ||
| 10 | 0.3 | 0.17 | Water Dipping | |
| 40 | 0.5 | 0.17 | Water Dipping | |
| 40 | 0.75 | 0.17 | Oil | |
| 63 | 1.4 | 0.17 | Water Dipping |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part number |
| Zeiss 90 HE LED | DAPI | 370-400 | 405 | 410-440 | |
| FITC | 450-488 | 493 | 499-529 | ||
| AF5686 | 540-570 | 575 | 580-604 | ||
| AF647 | 615-643 | 653 | 659-759 | ||
|
Zeiss 38 HE |
GFP, FITC, AF488 | 450-490 | 495 | 500-550 | |
| Zeiss 63 HE | AF568, mCherry, TRITC | 560-584 | 590 | 598-660 |
Light source (epi)
Colibri’ 7- LED fluorescent light (385/30, 423/44, 469/38, 511/44, 555/30, 590/27, 631/333)
Laser lines
355nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm
Detectors and Cameras
Hamamatsu ORCA-Flash-4.0 V3 (Monochrome for fluorescence imaging) GaAsP and AiryScan
Location
NMJ Facility, Cookson Building, Dental School, Room G33
Medical School
Newcastle University
Newcastle upon Tyne
NE2 4HH
Enter your postcode or address to find directions
Booking
Find out how to book this microscope.
Booking information
- The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.