Bioimaging Unit

Zeiss LSM880

Zeiss LSM880

Modalities: invert, confocal, live cell, airyscan

Overview

The Zeiss LSM 880 with Airyscan Fast is a confocal laser scanning microscope able to generate three-dimensional images of large specimen at super-resolution.

The system consists of an inverted microscope suitable for live cell imaging as well as fixed samples. 

The Airyscan system incorporates a novel spatial detector, the ‘Airyscan detector’, that increases spatial resolution 1.7x in each dimension (140 nm lateral and 400 nm axial resolution) or have the capabilities to achieves 4x faster temporal super-resolution imaging and requires 8x lower laser power while increasing the sensitivity, especially suited for dynamic life cell imaging. Due to the full environmental camber to control temperature and CO2 this microscope is perfect for life cell imaging as well as temperature sensitive studies. 

Imaging modes

  • Multipoint x,y with tiling (2D)
  • Volume x,y,z (3D)
  • Fluorescence
  • DIC
  • Time-lapse x,y,z,t (4D)
  • Z-stack and multi-color imaging of cell cultures, living sections, organs.
  • Imaging + electrophysiology combining mode 
  • SuperResolution (AiryScan) 

 

LSM 880 CfL

Specification

Here you find the specification for this microscope.

Objectives

Magnification

NA

Coverslip

Other modes

Immersion
10 0.45 0.17   Air
20 0.8  0.17   Air
40 1.2 0.17  W Korr FCS Water
40 1.3 0.17  DIC UV-IR Oil
63 1.4 0.17  DIC Oil

 

 

 

 

 

 

 

 

Filters (epi)

Cube

Example fluorophores

Excitation

Dichroic

Emission

Part number
Zeiss 49 DAPI, Hoechst 335-385  395 420-470   

Zeiss 38 HE

 GFP, FITC, AF488 450-490 495 500-550  
 Zeiss 43 HE CY5, AF647  625-645 660 675-715  

 

 

 

 

 

 

Light source (epi)

HXP120 - Broad spectrum fluorescent light

Laser lines

355nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm

Detectors

GaAsP and AiryScan

Location

West Wing, International Centre for Life, room B160

Newcastle University
International Centre for Life
Central Parkway
Newcastle upon Tyne
NE1 3BZ

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use. 
Make a Booking

SOP

Zeiss 880 (CfL) SOP

Training Videos

Carl Zeiss LSM 880

Airyscan and AiryscanFAST

Zen black - Tiling, Positions and Time series