Bioimaging Unit

Zeiss AxioImager (System 5)

Zeiss AxioImager with Apotome (System 5)

Modalities: upright, widefiled

Overview

The Zeiss AxioImager (system 5) is a high quality widefield imaging system.

The Zeiss AxioImager is a high quality imaging system that offers three modes of operation:

  • Brightfield transmitted light
  • Widefield fluorescence
  • Apotome structured illumination (confocal-like) fluorescence

The system utilises two separate camera systems for colour and monochrome imaging.

The Apotome (structured illumination) mode allows users to collect confocal-like images and ‘Z’ stacks, which can subsequently be projected as 3D images (stereo anaglyphs) and movies.

A range of fluorescent filters are available which cover the commonly used dyes:

  • DAPI/GFP/Cy3/Cy5
  • mPlum
  • Cy7

the illumination source is a wide spectrum alignment free LED (colibri) system.

The microscope has as an autofocus system and a precision motorised stage which enables users to automatically acquire tiled images from large specimens (or from an entire slide) and stitch them together post capture. 

Analysis of data/images can be carried out using the Zen blue software which includes quantification and colocalisation modules. 

Imaging modes

  • Wide field fluorescence
  • Brightfield
  • Multi-point x,y (2D) with tiling
  • Volume x,y,z (3D)
  • Structured illumination (Apotome, fluorescence only)
  • Phase contrast
  • DIC 

Specification

Here you can find the specifications for this microscope.

Objectives

Magnification NA Coverslip Other modes Immersion
2.5 0.075     Air
5 0.16 0.17 Ph1 Air
10 0.45 0.17 Ph1 Air
20 0.8 0.17 Ph1 Air
40 1.3 0.17 Ph2 Oil
63 1.4 0.17 Ph3 Oil

 

 

 

 

 

 

 

 

 

Filters (epi)

Cube

Example fluorophores

Excitation

Dichroic

Emission

Part number
Zeiss 90 DAPI, GFP,Cy3,Cy5 BP 385/30
BP 469/38
BP 555/30
BP 631/33		 QBS 405 + 493 + 575 + 653 QBP 425/30+514/30+592/25+709/100  489090-9110-000
Zeiss 64 mplum BP 587/25 (HE) FT 605 (HE) BP 647/70 (HE)  489064-0000-000
Shemrock Cy7          

Light source (epi)

Colibri 7- LED fluorescent light (365,470,555,625)

Detectors (cameras)

Zeiss Axiocam 503 (Monochrome for fluorescence imaging)

Zeiss Axiocam 105 (Colour for brightfield HC)

Location

Biomedicine West Wing, International Centre for Life, room B162

Newcastle University
International Centre for Life
Central Parkway
Newcastle upon Tyne
NE1 3BZ

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use. 
Make a booking

Training

Here you find useful training videos for the system.

Zen Blue software tutoial. 

Explore Zen.

SOP

Zeiss AxioImager (Zen Blue)