Zeiss AxioObserver Z1
Zeiss AxioObserver Z1
Modalities: inverted, widefiled
Overview
The Zeiss AxioObserver Z1 is a high quality widefiled imaging system specific for live imaging.
The Zeiss AxioObserver is a high quality imaging system that offers two modes of operation:
- Brightfield transmitted light
- Widefield fluorescence
The system utilises two separate camera systems for colour and monochrome imaging.
A range of fluorescent filters are available which cover the commonly used dyes:
- DAPI/GFP/mCherry
- Red Fluorescent protein
- AF546
- GFP
- DAPI
the illumination source is a wide spectrum alignment free LED (colibri) system.
The microscope has as an autofocus system and a precision motorised stage which enables users to automatically acquire tiled images from large specimens (or from an entire slide) and stitch them together post capture.
Analysis of data/images can be carried out using the Zen blue software which includes quantification and colocalisation modules.
Imaging modes
- Wide field fluorescence
- Brightfield
- Multi-point x,y (2D) with tiling
- Phase contrast
- DIC
Specification
Here you can find the specifications for this microscope.
Objectives
| Magnification | NA | Coverslip | Other modes | Immersion |
| 5 | 0.15 | 0.17 | Ph1 | Air |
| 10 | 0.3 | 0.17 | Ph1 | Air |
| 20 | 0.4 | 0.17 | Korr | Air |
| 40 | 0.6 | 0.17 | Korr | Air |
| 40 | 1.3 | 0.17 | Ph3 | Oil |
| 63 | 1.4 | 0.17 | Ph3 | Oil |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part number |
| Zeiss 92 | DAPI,GFP,mCherry | BP 385/30 BP 469/38 BP 591/27 |
TBS 405+493+610 | BP 425/30 BP 525/50 (HE) BP 649/65 |
489092-9010-000 |
| Zeiss 63 | RFP | BP 572/25 (HE) | FT 590 (HE) | BP 629/62 (HE) | 489063-0000-000 |
| Zeiss 15 | AF546 | BP 546/12 | FT 580 | LP 590 | 488015-0000-000 |
| Zeiss 13 | GFP | BP 470/20 | FT 495 | BP 505-530 | 488013-0000-000 |
| Zeiss 49 | DAPI | G 365 | FT 395 | BP 445/50 | 488049-9901-000 |
Light source (epi)
Colibri 2- LED fluorescent light (365,470,555,625)
Detectors (cameras)
Zeiss Axiocam 503 (Monochrome for fluorescence imaging)
Zeiss Axiocam 105 (Colour for brightfield HC)
Location
Biomedicine West Wing, International Centre for Life, room B162
Newcastle University
International Centre for Life
Central Parkway
Newcastle upon Tyne
NE1 3BZ
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Booking
Find out how to book this microscope.
Booking information
- The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.