Overview

The Zeiss AxioExaminer is an upright microscope compatible with electrophysiology techniques.

This equipment was funded by [Wellcome Trust, 2017], please reference the funder and [208339/Z/17/Z], in your publication.

This system offers a high quality imaging system for fluorescent imaging combined on an electrophysiology platform for combined experiments. It has superabundant space for specimen and experimental tools. 

Imaging modes

• Brightfield transmitted light (phase and DIC) 
• Wide field fluorescence 
• Imaging + electrophysiology combining mode  

Specification

Here you can find the specification for this microscope.

Objectives

Magnification	NA	Coverslip	Other modes	Immersion
Currently in use:
5	0.16			Air
10	0.45	0.17	DIC	Water Dipping
40	1	0.17	DIC	Water Dipping
40	0.75	0.17		Water Dipping
Additional available lenses:
2.5	0.12	0.17		Air
10	0.3			Water Dipping
10	0.5			Water Dipping
63	1.4			Oil
63	0.9	0.17	DIC	Water Dipping

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Filters (epi)

Cube	Example fluorophores	Excitation	Dichroic	Emission	Part number
Zeiss 90 HE LED	DAPI	370-400	405	410-440
	FITC	450-488	493	499-529
	AF5686	540-570	575	580-604
	AF647	615-643	653	659-759
Zeiss 38 HE	GFP, FITC, AF488	450-490	495	500-550
Zeiss 63 HE	AF568, mCherry, TRITC	560-584	590	598-660

Light source (epi)

Colibri’ 7- LED fluorescent light (385/30, 423/44, 469/38, 511/44, 555/30, 590/27, 631/333)

Detectors (cameras)

Hamamatsu ORCA-Flash-4.0 V3 (Monochrome for fluorescence imaging)

Location

NMJ Facility, Cookson Building, G33

Medical School
Newcastle University
Newcastle upon Tyne
NE2 4HH

Booking

Find out how to book this microscope.

 Booking information

• The minimal booking time for this system is 30 minutes. 
• Time increments of 15 minutes are permitted. 
• Bookings can be cancelled with no charge up to an hour before scheduled use.