Dr Luisa Wakeling
- Email: email@example.com
- Address: School of Dental Sciences
Newcastle upon Tyne
Stage 1 Director BDS programme
Chair of the Board of Examiners for Stage 1 BDS
Dental School Student-Staff Committee staff facilitator
Dental Education Research Group lead
Area of expertise
Nutritional epigenetics and ageing
Fellowship of the HEA (UKPSF D2) by completion of CASAP module, APP
National teaching award by completion of CASAP module, DST
BBSRC funded Ph.D. in Molecular Nutrition and Biochemistry, Institute for Cell and Molecular Biosciences. Supervisor: Professor Dianne Ford. Title of thesis: Genetic influences on inter-individual variation in soyabean isoflavone absorption and metabolism.
BSc Hons Pharmacology, Newcastle University
Introduction to Teaching and Learning in Higher Education (part A and B)
FMS Education Research Development and Practice grants:
Student induction to the University- does it work? December 2015 with L Ferrie, C Guilding, R Valentine and A Schartner
Participatory research with students and members of the public: How can we best evaluate innovative teaching about ageing? June 2016 with R Gilroy and E Tullo.
Evaluation of Context Café, a session which allows Stage 2 Biochemistry/ Genetics students to contextualise learning in the Degree Programme and beyond. June 2017 with J Brown and J Stewart.
ULTSEC Innovation awards:
Context Café: course integration sessions to engage Dental Surgery undergraduates with the basic sciences captured by notebook technology. May 2015 with M Vernon
Development of professional learning through student representation. May 2017 with M Thomason, G Watkins, J Barton and M Lintern
RANK prize vacation studentship February 2015 Modulation of gustatory receptor gene expression by an inflammatory immune response.
BRC- NIHR PhD Studentship September 2017. An investigation of the credentials of a novel, age-related biomarker for periodontitis. J Taylor, R Valentine, L Wakeling and P Preshaw.
Google scholar: Click here.
My main research interest is in the effect of nutrition on epigenetics (modifications to the genome such as DNA methylation) and ultimately the effect this has in healthy ageing and lifespan.
My research focuses on dietary polyphenols; from studying genetic differences that may influence variation in polyphenol metabolism, to investigating the ability of these polyphenols to mimic the beneficial effects seen with dietary restriction on ageing in endothelial and epithelial cell models.
A recent model organism I have been exploring to assess the effect of diet on DNA methylation and lifespan is the honeybee. Honeybees have similar methylation machinery to humans and it has been shown that a change in diet early in a bee’s life can dramatically alter the life outcome of the bee (lifespan and reproductive capacity are hugely altered); changes in DNA methylation also coincide with these dietary effects. We have shown that a protein, Sir2, which is involved in the lifespan response to dietary restriction, is also affected in the honeybee by diet. I am also interested in the taste receptor expression in the honeybee and bumble bee and the response to dietary interventions.
Arriving in the Dental School my research has also expanded to investigation the effect of inflammation on taste receptor gene expression using cell line models and the honeybee. I also investigate the effect of nutritional compounds such as resveratrol and gensitein on the expression and methylation of negative regulators of inflammatory processes.
I have also contributed to work investigating the ability of the dietary polyphenol, genistein to convert white adipocyte to beige adipocytes.
My work has also included the development of an accurate model for renal drug toxicity by applying flow across a proximal tubule epithelial cell model and assessing the regulation of monolayer formation, drug transporter expression and the uptake and flux of potential nephrotoxic drugs.
Member of Human Nutrition Research Centre (http://www.ncl.ac.uk/hnrc/) and Epithelial Research Group (http://research.ncl.ac.uk/epithelia/), Member of the Royal Society of Biology, Member of the British Society for Research in to Ageing, Member of the European Nutrigenomics Organisation.
Education Research interests:
Context Café: course integration session to engage Dental Surgery undergraduates with the basic sciences captured by notebook technology. L Wakeling and M Vernon (NUIT).
Students on student induction: a cross-disciplinary case study. EQUATE. with L Ferrie, C Guilding, A Schartner, R Valentine.
Opportunity for professional learning through student representation. L Wakeling, M Thomason and G Watkins (SU).
Involving older people in the design, development and delivery of an innovative module on ageing for undergraduates. E Tullo (SME), L Wakeling (Dental Sciences) and L Greaves (ICM).
Member of FMS Education Research Development and Practice
Member of the Dental Education Research Group
Member of Equal Acclaim for Teaching Excellence
Stage 1 and 2 Dental Surgery BDS programme, School of Dental Sciences:
Major contributor Cell Biology (Stage 1) and Blood theme-lead for Dental Physiology. I also teach across Cell Physiology (Stage 1), Metabolism and Homeostasis (Stage 1), Microbiology for Dentists, leading the Clinical Trial project (Stage 2), Pharmacology (Stage 2) and Nutrition and Diet (Stage 2).
Course lead for Orientation and Study Skills (Stage 1).
Including development and delivery of Context café – a basic sciences integration method.
Wider university teaching:
Seminar leader for Stage 1 Pharmacology, School of Biomedical Sciences
Lecturer: BGM3063 Epigenetics
BGM3063 Context café
Assessment lead and teacher for Newcastle University Ageing Generation Module (NUAGE) AGE2000
Supervision and training in research of undergraduate, MSci, MRes and PhD students
Supervision and training in education research of NWE student internships
- Simcock NK, Wakeling LA, Ford D, Wright GA. Effects of age and nutritional state on the expression of gustatory receptors in the honeybee (Apis mellifera). PLOS One 2017, 12(4), e0175158.
- Aziz SA, Wakeling LA, Miwa S, Alberdi G, Hesketh JE, Ford D. Metabolic programming of a beige adipocyte phenotype by genistein. Molecular Nutrition and Food Research 2017, 61(2), 1600574.
- Wakeling L, Jakubovics N, McHanwell S, Stewart J. Challenging the basic sciences 'learn and forget' culture. Medical Education 2016, 50(5), 578-579.
- Tullo E, Greaves L, Wakeling L. Involving older people in the design, development, and delivery of an innovative module on aging for undergraduate students. Educational Gerontology 2016, 42(10), 698-705.
- Hardyman J, Tyson J, Jackson K, Aldridge C, Cockell S, Wakeling L, Valentine RA, Ford D. Zinc sensing by metal-responsive transcription factor 1 (MTF1) controls metallothionein and ZnT1 expression to buffer the sensitivity of the transcriptome response to zinc. Metallomics 2016, 8(3), 337-343.
- McKay JA, Wakeling LA. Nutrition, epigenetics and ageing. In: Deliminda Neves, ed. Anti-Ageing Nutrients Evidence-Based Prevention of Age-Associated Diseases. Wiley-Blackwell Publishing Ltd, 2015, pp.133-147.
- Wakeling LA, Ions LJ, Escolme SM, Cockell SJ, Su T, Dey M, Hampton EV, Jenkins G, Wainwright LJ, McKay JA, Ford D. SIRI1 effects DNA methylation of polycomb protein target genes, a hotspot of the epigenetic shift observed in ageing. Human Genomics 2015, 9, 14.
- Aziz S, Wakeling L, Hesketh J, Ford D. Genistein promotes a gene expression profile characteristic of brown rather than white adipocytes and increases Sirt1 expression in mouse NIH3T3-L1 cells. In: Experimental Biology Meeting 2014. 2014, San Diego, CA: Federation of American Societies for Experimental Biology.
- Paoli PP, Wakeling LA, Wright GA, Ford D. The dietary proportion of essential amino acids and Sir2 influence lifespan in the honeybee. AGE 2014, 36, 1239-1247.
- Hardyman JEJ, Ogo OA, Tyson J, Wakeling LA, Valentine RA, Ford D. DNA methylation of the zinc transcriptional regulatory element and its potential contribution to zinc dyshomeostasis in ageing. In: Nutrition Society Annual Summer Meeting. 2013, Newcastle upon Tyne, UK: Cambridge University Press.
- Escolme SM, Wakeling LA, Alatawi F, Valentine R, Ford D. Does resveratrol act independently of SIRT1 to affect genes relevant to ageing?. In: Nutrition Society Annual Summer Meeting. 2013, Newcastle upon Tyne, UK: Cambridge University Press.
- Alberdi G, Wakeling L, Lasa A, Rodriguez VM, Macarulla MT, Ford D, Portillo MP. Effects of resveratrol on the methylation of lipogenic enzyme genes in 3T3-L1 adipocytes. In: 20th International Congress of Nutrition. 2013, Granada, Spain: Karger.
- Ions LJ, Wakeling LA, Bosomworth HJ, Hardyman JEJ, Escolme SM, Swan DC, Valentine RA, Mathers JC, Ford D. Effects of Sirt1 on DNA methylation and expression of genes affected by dietary restriction. Age 2013, 35(5), 1835-1849.
- Wakeling LA, Ford D. Polymorphisms in genes involved in the metabolism and transport of soy isoflavones affect the urinary metabolite profile in premenopausal women following consumption of a commercial soy supplement as a single bolus dose. Molecular Nutrition & Food Research 2012, 56(12), 1794-1802.
- Wakeling LA, Alatawi F, Ions LJ, Hesketh J, Ford D. SIRT1-independent epigenetic effects of resveratrol mediated through the estrogen receptor. In: Experimental Biology Meeting. 2012, San Diego, CA: Federation of American Societies for Experimental Biology.
- Ford D, Ions LJ, Alatawi F, Wakeling LA. The potential role of epigenetic responses to diet in ageing. In: Proceedings of the Nutrition Society: A joint meeting of the Nutrition Society and the UK Molecular Epidemiology Group. 2011, London: Cambridge University Press.
- Ions L, Wakeling L, Ford D. Can soyabean isoflavones mimic the effects of energy restriction on healthy ageing?. Nutrition Bulletin 2009, 34(3), 303-308.
- Wakeling LA, Ions LJ, Ford D. Could Sirt1-mediated epigenetic effects contribute to the longevity response to dietary restriction and be mimicked by other dietary interventions?. Age 2009, 31(4), 327-341.
- Wakeling L, Ford D. Genetic influences on interindividual variability in soybean isoflavone absorption and metabolism. In: International Research Conference on Food, Nutrition, and Cancer. 2007, Washington, DC: Journal of Nutrition, American Society for Nutrition.
- Wakeling LA, McGarr EF, Ford D. The association of single nucleotide polymorphisms with beta-glucosidase activity in oral epithelium. In: Proceedings of the Nutrition Society. 2006, Aberdeen, Scotland: Cambridge University Press.