Staff Profile

Qualifications

BSc Molecular Biology (University of Glasgow)
PhD Molecular Biology (University of Edinburgh)

Research Interests

My laboratory is interested in the regulation of the oxidative stress response and the regulation of the cell division cycle in eukaryotes. Specifically we are investigating the regulation of gene expression important for these cellular events and exploring the signal transduction pathways which regulate this gene expression. We use the yeast Saccharomyces cerevisiae and Schizosaccharomyces pombe as model organisms to study these regulatory mechanisms as many aspects of these cellular processes are conserved in all eukaryotes. This choice of organisms allows us to apply a wide range of experimental tools including classical genetic and biochemical approaches as well as standard molecular biology techniques.

• Articles

  • Gal C, Cochrane GA, Morgan BA, Rallis C, Bähler J, Whitehall SK. The longevity and reversibility of quiescence in Schizosaccharomyces pombe are dependent upon the HIRA histone chaperone. Cell Cycle 2023, 22(17), 1921-1936.
  • Cao M, Day AM, Galler M, Latimer HR, Byrne DP, Foy TW, Dwyer E, Bennett E, Palmer J, Morgan BA, Eyers PA, Veal EA. A peroxiredoxin-P38 MAPK scaffold increases MAPK activity by MAP3K-independent mechanisms. Molecular Cell 2023, 83(17), 3140-3154.e7.
  • Day AM, Smith DA, Ikeh MAC, Haider M, Herrero-de-Dios CM, Brown AJP, Morgan BAM, Erwig LP, MacCallum DM, Quinn J. Blocking two-component signalling enhances Candida albicans virulence and reveals adaptive mechanisms that counteract sustained SAPK activation. PLoS Pathogens 2017, 13(1), e1006131.
  • Hewitt G, Carroll B, Sarallah R, Correia-Melo C, Ogrodnik M, Nelson G, Otten EG, Manni D, Antrobus R, Morgan BA, von Zglinicki T, Jurk D, Seluanov A, Gorbunova V, Johansen T, Passos JF, Korolchuk VI. SQSTM1/p62 mediates crosstalk between autophagy and the UPS in DNA repair. Autophagy 2016, 12(10), 1917-1930.
  • Patterson M, McKenzie C, Smith D, da Silva Dantas A, Sherston S, Veal EA, Morgan B, MacCallum D, Erwig LP, Quinn J. Ybp1 and Gpx3 Signaling in Candida albicans Govern Hydrogen Peroxide-Induced Oxidation of the Cap1 Transcription Factor and Macrophage Escape. Antioxidants & Redox Signaling 2013, 19(18), 2244-2260.
  • Flanagan MD, Whitehall SK, Morgan BA. An Atg10-like E2 enzyme is essential for cell cycle progression but not autophagy in Schizosaccharomyces pombe. Cell Cycle 2013, 12(2), 271-277.
  • Brown JD, Day AM, Taylor SR, Tomalin LE, Morgan BA, Veal EA. A Peroxiredoxin Promotes H₂O₂ Signaling and Oxidative Stress Resistance by Oxidizing a Thioredoxin Family Protein. Cell Reports 2013, 5(5), 1425-1435.
  • Darieva Z, Han N, Warwood S, Doris KS, Morgan BA, Sharrocks AD. Protein Kinase C Regulates Late Cell Cycle-Dependent Gene Expression. Molecular and Cellular Biology 2012, 32(22), 4651-4661.
  • Doris KS, Rumsby EL, Morgan BA. Oxidative Stress Responses Involve Oxidation of a Conserved Ubiquitin Pathway Enzyme. Molecular and Cellular Biology 2012, 32(21), 4472-4481.
  • Day AM, Brown JD, Taylor SR, Rand JD, Morgan BA, Veal EA. Inactivation of a peroxiredoxin by hydrogen peroxide is critical for thioredoxin-mediated repair of oxidized proteins and cell survival. Molecular Cell 2012, 45(3), 398-408.
  • Quinn J, Malakasi P, Smith DA, Cheetham J, Buck V, Millar J, Morgan BA. Two-component mediated peroxide sensing and signal transduction in fission yeast. Antioxidants & Redox Signaling 2011, 15(1), 153-165.
  • Bruce CR, Smith DA, Rodgers D, Dantas AD, MacCallum DM, Morgan BA, Quinn J. Identification of a Novel Response Regulator, Crr1, That Is Required for Hydrogen Peroxide Resistance in Candida albicans. PLoS One 2011, 6(12), e27979.
  • Purtill FS, Whitehall SK, Williams ES, McInerny CJ, Sharrocks AD, Morgan BA. A homeodomain transcription factor regulates the DNA replication checkpoint in yeast. Cell Cycle 2011, 10(4), 664-670.
  • da Silva Dantas A, Patterson MJ, Smith DA, MacCallum DM, Erwig LP, Morgan BA, Quinn J. Thioredoxin regulates multiple hydrogen peroxide-induced signaling pathways in Candida albicans. Molecular and Cellular Biology 2010, 30(19), 4550-4563.
  • Darieva Z, Clancy A, Bulmer R, Williams E, Pic-Taylor A, Morgan BA, Sharrocks AD. A Competitive Transcription Factor Binding Mechanism Determines the Timing of Late Cell Cycle-Dependent Gene Expression. Molecular Cell 2010, 38(1), 29-40.
  • Oláhová M, Taylor SR, Khazaiphoul S, Wang J, Morgan BA, Matsumoto K, Blackwell TK, Veal EA. A redox-sensitive peroxiredoxin that is important for longevity has tissue- and stress-specific roles in stress resistance. Proceedings of the National Academy of Sciences 2008, 105(50), 19839-19844.
  • Veal EA, Day AM, Morgan BA. Hydrogen Peroxide Sensing and Signaling. Molecular Cell 2007, 26(1), 1-14.
  • Bozonet SM, Findlay VJ, Day AM, Cameron J, Veal EA, Morgan BA. Oxidation of a eukaryotic 2-Cys peroxiredoxin is a molecular switch controlling the transcriptional response to increasing levels of hydrogen peroxide. Journal of Biological Chemistry 2005, 280(24), 23319-23327.
  • Bulmer R, Pic-Taylor A, Whitehall SK, Martin KA, Millar JBA, Quinn J, Morgan BA. The forkhead transcription factor Fkh2 regulates the cell division cycle of Schizosaccharomyces pombe. Eukaryotic Cell 2004, 3(4), 944-954.
  • Pic-Taylor A, Darieva Z, Morgan BA, Sharrocks AD. Regulation of cell cycle-specific gene expression through cyclin-dependent kinase-mediated phosphorylation of the forkhead transcription factor Fkh2p. Molecular and Cellular Biology 2004, 24(22), 10036-10046.
  • Smith DA, Nicholls S, Morgan BA, Brown AJP, Quinn J. A conserved stress-activated protein kinase regulates a core stress response in the human pathogen Candida albicans. Molecular Biology of the Cell 2004, 15(9), 4179-4190.
  • Veal EA, Findlay VJ, Day AM, Bozonet SM, Evans JM, Quinn J, Morgan BA. A 2-Cys peroxiredoxin regulates peroxide-induced oxidation and activation of a stress-activated MAP kinase. Molecular Cell 2004, 15(1), 129-139.
  • Veal EA, Ross SJ, Malakasi P, Peacock E, Morgan BA. Ybp1 is required for the hydrogen peroxide-induced oxidation of the Yap1 transcription factor. Journal of Biological Chemistry 2003, 278(33), 30896-30904.
  • Boros J, Lim F-L, Darieva Z, Pic-Taylor A, Harman R, Morgan BA, Sharrocks AD. Molecular determinants of the cell-cycle regulated Mcm1p-Fkh2p transcription factor complex. Nucleic Acids Research 2003, 31(9), 2279-2288.
  • Lim F-L, Hayes A, West AG, Pic-Taylor A, Darieva Z, Morgan BA, Oliver SG, Sharrocks AD. Mcm1p-induced DNA bending regulates the formation of ternary transcription factor complexes. Molecular and Cellular Biology 2003, 23(2), 450-461.
  • Darieva Z, Pic-Taylor A, Boros J, Spanos A, Geymonat M, Reece RJ, Sedgwick SG, Sharrocks AD, Morgan BA. Cell Cycle-Regulated Transcription through the FHA Domain of Fkh2p and the Coactivator Ndd1p. Current Biology 2003, 13(19), 1740-1745.
  • Smith DA, Mark Toone W, Chen D, Bahler J, Jones N, Morgan BA, Quinn J. The Srk1 protein kinase is a target for the Sty1 stress-activated MAPK in fission yeast. Journal of Biological Chemistry 2002, 277(36), 33411-33421.
  • Greenall A, Hadcroft AP, Malakasi P, Jones N, Morgan BA, Hoffman CS, Whitehall SK. Role of fission yeast Tup1-like repressors and Prr1 transcription factor in response to salt stress. Molecular Biology of the Cell 2002, 13(9), 2977-2989.
  • Veal EA, Mark Toone W, Jones N, Morgan BA. Distinct roles for glutathione S-transferases in the oxidative stress response in Schizosaccharomyces pombe. Journal of Biological Chemistry 2002, 277(38), 35523-35531.
  • Quinn J, Findlay VJ, Dawson K, Millar JB, Jones N, Morgan BA, Toone WM. Distinct Regulatory Proteins Control the Graded Transcriptional Response to Increasing H₂O₂ Levels in Fission Yeast Schizosaccharomyces pombe. Molecular Biology of the Cell 2002, 13(3), 805-816.
  • Buck V, Quinn J, Pino TS, Martin H, Saldanha J, Makino K, Morgan BA, Millar JBA. Peroxide sensors for the fission yeast stress-activated mitogen-activated protein kinase pathway. Molecular Biology of the Cell 2001, 12(2), 407-419.
  • Ross SJ, Findlay VJ, Malakasi P, Morgan BA. Thioredoxin peroxidase is required for the transcriptional response to oxidative stress in budding yeast. Molecular Biology of the Cell 2000, 11(8), 2631-2642.
  • Raitt DC, Johnson AL, Erkine AM, Makino K, Morgan B, Gross DS, Johnston LH. The Skn7 response regulator of Saccharomyces cerevisiae interacts with Hsf1 in vivo and is required for the induction of heat shock genes by oxidative stress. Molecular Biology of the Cell 2000, 11(7), 2335-2347.
  • Pic A, Lim F-L, Ross SJ, Veal EA, Johnson AL, Sultan MRA, West AG, Johnston LH, Sharrocks AD, Morgan BA. The forkhead protein Fkh2 is a component of the yeast cell cycle transcription factor SFF. EMBO Journal 2000, 19(14), 3750-3761.
  • Bouquin N, Johnson AL, Morgan BA, Johnston LH. Association of the cell cycle transcription factor Mbp1 with the Skn7 response regulator in budding yeast. Molecular Biology of the Cell 1999, 10(10), 3389-3400.
  • Sedgwick S.G., Taylor I.A., Adam A.C., Spanos A., Howell S., Morgan B.A., Treiber M.K., Kanuga N., Banks G.R., Foord R. and Smerdon S.J. Structural and functional architecture of the yeast cell-cycle transcription factor Swi6. Journal of Molecular Biology 1998, 281, 763-775.
  • W. M. Toone, S. Kuge, M. Samuels, B. A. Morgan, T. Toda and N. Jones. Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (Exportin) and the stress-activated MAP kinase Sty1/Spc1. Genes & Development 1998, 12, 1453-1463.
  • Machado A.K., Morgan B.A. and Merrill G.F. Thioredoxin reductase-dependent inhibition of MCB cell cycle box activity in Saccharomyces cerevisiae. Journal of Biological Chemistry 1997, 272, 17045-17054.
  • B. A. Morgan, G. R. Banks, W. M. Toone, D. Raitt, S. Kuge and L. H. Johnston. The Skn7 response regulator controls gene expression in the oxidative stress response of the budding yeast Saccharomyces cerevisiae. Embo Journal 1997, 16, 1035-1044.
  • Shieh J-C., Wilkinson M., Buck V., Morgan B.A., Makino K. and Millar J.B.A. The Mcs4 response regulator co-ordinately controls the stress activated Wak1-Wis1-Sty1 MAP kinase pathway and fission yeast cell cycle. Genes and Development 1997, 11, 1008-1022.
  • W. M. Toone, B. L. Aerne, B. A. Morgan and L. H. Johnston. Getting started: Regulating the initiation of DNA replication in yeast. Annual Review of Microbiology 1997, 51, 125-149.

• Book Chapter

  • Morgan BA, Veal EA. Typical 2-Cys peroxiredoxins in Yeast. In: Flohe, L; Harris, R, ed. Peroxiredoxin Systems: Structures and Functions. New York: Springer, 2007, pp.253-265.

• Conference Proceedings (inc. Abstracts)

  • Smith DA, MacCallum DM, Morgan BA, Quinn J. Ypd1-mediated two-component signalling in Candida albicans. In: 18th Congress of the International Society for Human and Animal Mycology. 2012, Berlin, Germany: Wiley-Blackwell Verlag GmbH.
  • Veal E, Olahova M, Taylor SR, Khazaipoul S, Wang J, Crook H, Day A, Morgan B, Matsumoto K, Blackwell K. Redox-sensitive peroxiredoxins play multiple roles in the oxidative stress resistance of Caenorhabditis elegans. In: 31st Annual Meeting of the United Kingdom Environmental Mutagen Society. 2009, University of Northumbria, Newcastle upon Tyne: Oxford University Press.

• Letter

  • Darieva Z, Bulmer R, Pic-Taylor A, Doris KS, Geymonat M, Sedgwick SG, Morgan BA, Sharrocks AD. Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Nature 2006, 444(7118), 494-498.

• Reviews

  • Veal EA, Underwood ZE, Tomalin LE, Morgan BA, Pillay CS. Hyperoxidation of Peroxiredoxins: Gain or Loss of Function?. Antioxidants and Redox Signaling 2018, 28(7), 574-590.
  • Veal EA, Tomalin LE, Morgan BA, Day AM. The fission yeast Schizosaccharomyces pombe as a model to understand how peroxiredoxins influence cell responses to hydrogen peroxide. Biochemistry Society Transactions 2014, 42(4), 909-916.
  • Smith DA, Morgan BA, Quinn J. Stress signalling to fungal stress-activated protein kinase pathways. FEMS Microbiology Letters 2010, 306(1), 1-8.
  • Toone WM, Morgan BA, Jones N. Redox control of AP-1-like factors in yeast and beyond. Oncogene 2001, 20(19), 2336-2346.