Staff Profile

Qualifications

2010 to 2012: University of Glasgow. Postgraduate Certificate in Academic Practice (PgCAP; 60 credits). Principle and practices of Learning and Teaching in Higher Education (30 credits); Learning and Teaching in Higher Education: Disciplinary and institutional orientations (30 credits).  

2000 to 2004: Newcastle University. Ph.D. from the School of Medicine. Thesis entitled: ‘Investigations of Avian Gut Antimicrobials’ (Submission in June 2004, defended August 2004 with minor corrections). Supervised by Dr Judith Hall and Professor Harry Gilbert.

1996 to 2000: Newcastle University. BSc. (upper 2^nd class) in Applied Biology with honours in Biotechnology. One-year industrial placement at AstraZeneca (Billingham, UK).

Previous Employment

2017 to 2020: Senior Lecturer in Oral Biosciences. Oral Sciences Research Group, University of Glasgow Dental School, School of Medicine, Dentistry and Nursing, University of Glasgow, 378 Sauchiehall Street, Glasgow, G2 3JZ.

2010 to 2016: Lecturer in Immunology. Oral Sciences Research Group, University of Glasgow Dental School, School of Medicine, Dentistry and Nursing, University of Glasgow, 378 Sauchiehall Street, Glasgow, G2 3JZ.

2006 to 2010: Postdoctoral Research Associate. Oral Biology, School of Dental Sciences, Newcastle University, Framlington Place, Newcastle upon Tyne, Tyne and Wear, NE2 4BW.

2005 to 2006: Postdoctoral Research Associate. Academic Rheumatology, Division of Genomic Medicine, The Medical School, University of Sheffield, Beech Hill Road, Sheffield, South Yorkshire, S10 2RX. 



Overview

Chris is interested in how microorganisms can initiate an inflammatory response and the host-pathogen crosstalk which is an important determinant of disease outcome. Acetylcholine has been found to play an important role in cross-communication between host and pathogen. Both eukaryotes and prokaryotes can synthesise and respond to acetylcholine. Work from his group, and others, have demonstrated that acetylcholine plays an important role in modulation of immune responses and microbial pathogenicity. Chris’ research group explore the role of cholinergic enzymes and receptors in health and disease and investigate whether targeting these molecules may lead to beneficial therapeutic outcomes. 

Current Group Members

Alina Goldberg (Postdoctoral research associate)

Mukul Verma (PhD student)

Saeed Alqahtani (PhD student - Glasgow University)

Maria Tumelty (MD student - Glasgow University)

Kimberley Ladner (MRes Immunobiology)

Co-supervisory role

Nontawat Chuinsiri (PhD)

Jamie Coulter (PhD)

Mahwash Malik (PhD)

Israa Asker (PhD)

Journal Editor

Critical Reviews in Microbiology

Frontiers in Microbiology

Committee Positions

British Society of Oral and Dental Research (BSODR) Honorary Assistant Secretary

Engagement

STEM Ambassador

Senior Roles

Stage 1 BDS Director

Course Lead

DEN1108: Induction, Orientation and Study skills

DEN2106: Immunology and Healthcare

MMB8051: MRes Oral and Dental Sciences

Courses Taught

DEN1108: Induction, Orientation and Study Skills

DEN2106: Immunology and Healthcare

MMB8051: MRes Oral and Dental Sciences

DEN2108: Microbiology for Dentistry

PGD1003: MClinDent

PHM2103 and PHM2101: Pharmacy

BMS3021: Immunology of Health and Disease

PSC3013: Physiology of the Gastrointestinal Tract

• Articles

  • Allison JR, Dowson C, Jakubovics NS, Nile C, Durham J, Holliday R. Waterline Disinfectants Reduce Dental Bioaerosols: A Multitracer Validation. Journal of Dental Research 2022, 101(10), 1198-1204.
  • Brown JL, Townsend E, Short RD, Williams C, Woodall C, Nile CJ, Ramage G. Assessing the inflammatory response to in vitro polymicrobial wound biofilms in a skin epidermis model. npj Biofilms and Microbiomes 2022, 8(1), 19.
  • McKloud E, Delaney C, Sherry L, Kean R, Williams S, Metcalfe R, Thomas R, Richardson R, Gerasimidis K, Nile CJ, Williams C, Ramage G. Recurrent vulvovaginal candidiasis: A dynamic interkingdom biofilm disease of candida and lactobacillus. mSystems 2021, 6(4), e00622-21.
  • Holliday R, Allison JR, Currie CC, Edwards DC, Bowes C, Pickering K, Reay S, Durham J, Lumb J, Rostami N, Coulter J, Nile CJ, Jakubovics N. Evaluating contaminated dental aerosol and splatter in an open plan clinic environment: Implications for the COVID-19 pandemic. Journal of Dentistry 2021, 105, 103565.
  • Allison JR, Currie CC, Edwards DC, Bowes C, Coulter J, Pickering K, Kozhevnikova E, Durham J, Nile CJ, Jakubovics N, Rostami N, Holliday R. Evaluating aerosol and splatter following dental procedures: Addressing new challenges for oral health care and rehabilitation. Journal of Oral Rehabilitation 2021, 48(1), 61-72.
  • Kanmaz B, Lappin DF, Nile CJ, Buduneli N. Effects of smoking on non‐surgical periodontal therapy in patients with periodontitis stage III or IV, and grade C. Journal of Periodontology 2020, 91(4), 442-453.
  • Nile C, Falleni M, Cirasola D, Alghamdi A, Anderson OF, Delaney C, Ramage G, Ottaviano E, Tosi D, Bulfamante G, Morace G, Borghi E. Repurposing pilocarpine hydrochloride for treatment of Candida albicans infections. mSphere 2019, 4(1), e00689-18.
  • Delaney C, O'Donnell LE, Kean R, Sherry L, Brown JL, Calvert G, Nile CJ, Cross L, Bradshaw DJ, Brandt BW, Robertson D, Ramage G. Interkingdom interactions on the denture surface: Implications for oral hygiene. Biofilm 2019, 1, 100002.
  • Meric P, Buduneli N, Kanmaz B, Gurlek O, Comlekoglu E, Calvert G, Lappin DF, Nile C. Cholinergic signalling mechanisms and early implant healing phases in healthy versus generalized aggressive periodontitis patients: A prospective, case-control study. Journal of Clinical Periodontology 2019, 46(11), 1155-1163.
  • Alshanta OA, Shaban S, Nile CJ, McLean W, Ramage G. Candida albicans biofilm heterogeneity and tolerance of clinical isolates: Implications for secondary endodontic infections. Antibiotics 2019, 8(4), 204.
  • Apatzidou DA, Nile C, Bakopoulou A, Konstantinidis A, Lappin DF. Stem cell-like populations and immunoregulatory molecules in periodontal granulation tissue. Journal of Periodontal Research 2018, 53(4), 610-621.
  • Gurlek C, Ertugrul F, Nile C, Lappin DF, Buduneli N. Plaque accumulation and inflammation adjacent to restorations of amorphous calcium phosphate-containing composite in early childhood caries. Oral Health and Preventive Dentistry 2018, 16(5), 457-465.
  • Alalwan H, Nile CJ, Rajendran R, McKerlie R, Reynolds P, Gadegaard N, Ramage G. Nanoimprinting of biomedical polymers reduces candidal physical adhesion. Nanomedicine: Nanotechnology, Biology, and Medicine 2018, 14(3), 1045-1049.
  • Apatzidou DA, Iskas A, Konstantinidis A, Alghamdi AM, Tumelty M, Lappin DF, Nile CJ. Clinical associations between acetylcholine levels and cholinesterase activity in saliva and gingival crevicular fluid and periodontal diseases. Journal of Clinical Periodontology 2018, 45(10), 1173-1183.
  • Greetham D, Lappin DF, Rajendran R, O'Donnell L, Sherry L, Ramage G, Nile C. The application of phenotypic microarray analysis to anti-fungal drug development. Journal of Microbiological Methods 2017, 134, 35-37.
  • Alalwan H, Rajendran R, Lappin DF, Combet E, Shahzad M, Robertson D, Nile CJ, Williams C, Ramage G. The anti-adhesive effect of curcumin on Candida albicans biofilms on denture materials. Frontiers in Microbiology 2017, 8, 659.
  • Thomas S, Lappin DF, Spears J, Bennett D, Nile C, Riggio MP. Prevalence of feline calicivirus in cats with odontoclastic resorptive lesions and chronic gingivostomatitis. Research in Veterinary Science 2017, 111, 124-126.
  • Gurlek O, Gumusx P, Nile CJ, Lappin DF, Buduneli N. Biomarkers and bacteria around implants and natural teeth in the same individuals. Journal of Periodontology 2017, 88(8), 752-761.
  • O'Donnell LE, Alalwan HKA, Kean R, Calvert G, Nile CJ, Lappin DF, Robertson D, Williams C, Ramage G, Sherry L. Candida albicans biofilm heterogeneity does not influence denture stomatitis but strongly influences denture cleansing capacity. Journal of Medical Microbiology 2017, 66(1), 54-60.
  • Sherry L, Lappin G, O'Donnell LE, Millhouse E, Millington OR, Bradshaw DJ, Axe AS, Williams C, Nile CJ, Ramage G. Viable compositional analysis of an eleven species oral polymicrobial biofilm. Frontiers in Microbiology 2016, 7, 912.
  • Nile CJ, Apatzidou DA, Awang RA, Riggio MP, Kinane DF, Lappin DF. The effect of periodontal scaling and root polishing on serum IL-17E concentrations and the IL-17A:IL-17E ratio. Clinical Oral Investigations 2016, 20(9), 2529-2537.
  • O'Donnell LE, Smith K, Williams C, Nile CJ, Lappin DF, Bradshaw D, Lambert M, Robertson DP, Bagg J, Hannah V, Ramage G. Dentures are a Reservoir for Respiratory Pathogens. Journal of Prosthodontics 2016, 25(2), 99-104.
  • Rajendran R, Sherry L, Nile CJ, Sherriff A, Johnson EM, Hanson MF, Williams C, Munro CA, Jones BJ, Ramage G. Biofilm formation is a risk factor for mortality in patients with Candida albicans bloodstream infection-Scotland, 2012-2013. Clinical Microbiology and Infection 2016, 22(1), 87-93.
  • O'Donnell LE, Robertson D, Nile CJ, Cross LJ, Riggio M, Sherriff A, Bradshaw D, Lambert M, Malcolm J, Buijs MJ, Zaura E, Crielaard W, Brandt BW, Ramage G. The oral microbiome of denture wearers is influenced by levels of natural dentition. PLoS ONE 2015, 10(9), e0137717.
  • Lappin DF, Robertson D, Hodge P, Treagus D, Awang RA, Ramage G, Nile CJ. The influence of glycated hemoglobin on the cross susceptibility between type 1 diabetes mellitus and periodontal disease. Journal of Periodontology 2015, 86(11), 1249-1259.
  • Borghi E, Morace G, Borgo F, Rajendran R, Sherry L, Nile C, Ramage G. New strategic insights into managing fungal biofilms. Frontiers in Microbiology 2015, 6, 1077.
  • Malcolm J, Awang RA, Oliver-Bell J, Butcher JP, Campbell L, Adrados Planell A, Lappin DF, Fukada SY, Nile CJ, Liew FY, Culshaw S. IL-33 exacerbates periodontal disease through induction of RANKL. Journal of Dental Research 2015, 94(7), 968-975.
  • Rajendran R, Borghi E, Falleni M, Perdoni F, Tosi D, Lappin DF, O'Donnell L, Greetham D, Ramage G, Nile C. Acetylcholine protects against Candida albicans infection by inhibiting biofilm formation and promoting hemocyte function in a Galleria mellonella infection model. Eukaryotic Cell 2015, 14(8), 834-844.
  • Macpherson A, Zoheir N, Awang RA, Culshaw S, Ramage G, Lappin DF, Nile CJ. The alpha 7 nicotinic receptor agonist PHA-543613 hydrochloride inhibits Porphyromonas gingivalis-induced expression of interleukin-8 by oral keratinocytes. Inflammation Research 2014, 63(7), 557-568.
  • Jitprasertwong P, Jaedicke KM, Nile CJ, Preshaw PM, Taylor JJ. Leptin enhances the secretion of interleukin (IL)-18, but not IL-1B, from human monocytes via activation of caspase-1. Cytokine 2014, 65(2), 222-230.
  • Rajendran R, Sherry L, Lappin DF, Nile CJ, Smith K, Williams C, Munro CA, Ramage G. Extracellular DNA release confers heterogeneity in Candida albicans biofilm formation. BMC Microbiology 2014, 14(1), 303.
  • Sherry L, Rajendran R, Lappin DF, Borghi E, Perdoni F, Falleni M, Tosi D, Smith K, Williams C, Jones B, Nile CJ, Ramage G. Biofilms formed by Candida albicans bloodstream isolates display phenotypic and transcriptional heterogeneity that are associated with resistance and pathogenicity. BMC Microbiology 2014, 14, 182.
  • Ozcaka O, Buduneli N, Ceyhan BO, Akcali A, Hannah V, Nile C, Lappin DF. Is interleukin-17 involved in the interaction between polycystic ovary syndrome and gingival inflammation?. Journal of Periodontology 2013, 84(12), 1827-1837.
  • Sherry L, Millhouse E, Lappin DF, Murray C, Culshaw S, Nile CJ, Ramage G. Investigating the biological properties of carbohydrate derived fulvic acid (CHD-FA) as a potential novel therapy for the management of oral biofilm infections. BMC Oral Health 2013, 13(1), 47.
  • Gumus P, Buduneli E, Biyikoglu B, Aksu K, Sarac F, Nile C, Lappin D, Buduneli N. Gingival Crevicular Fluid, Serum Levels of Receptor Activator of Nuclear Factor‐κB Ligand, Osteoprotegerin, and Interleukin‐17 in Patients With Rheumatoid Arthritis and Osteoporosis and With Periodontal Disease. Journal of Periodontology 2013, 84(11), 1627-1637.
  • Nile CJ, Sherrabeh S, Ramage G, Lappin DF. Comparison of circulating tumour necrosis factor superfamily cytokines in periodontitis patients undergoing supportive therapy: A case-controlled cross-sectional study comparing smokers and non-smokers in health and disease. Journal of Clinical Periodontology 2013, 40(9), 875-882.
  • Nile CJ, Gillespie JI. Interactions Between Cholinergic and Prostaglandin Signaling Elements in the Urothelium: Role for Muscarinic Type 2 Receptors. Urology 2012, 79(1), 240.e17–240.e23.
  • Grol S, Nile CJ, Martinez-Martinez P, van Koeveringe G, de Wachter S, de Vente J, Gillespie JI. M-3 Muscarinic Receptor-Like Immunoreactivity in Sham Operated and Obstructed Guinea Pig Bladders. Journal of Urology 2011, 185(5), 1959-1966.
  • Nile CJ, de Vente J, Gillespie JI. Stretch independent regulation of prostaglandin E-2 production within the isolated guinea-pig lamina propria. BJU International 2010, 105(4), 540-548.
  • Nile CJ, Barksby E, Jitprasertwong P, Preshaw PM, Taylor JJ. Expression and regulation of interleukin-33 in human monocytes. Immunology 2010, 130(2), 172-180.
  • Howard A, Townes C, Milona P, Nile CJ, Michailidis G, Hall J. Expression and functional analyses of liver expressed antimicrobial peptide-2 (LEAP-2) variant forms in human tissues. Cellular Immunology 2010, 261(2), 128-133.
  • Barksby HE, Nile CJ, Jaedicke KM, Taylor JJ, Preshaw PM. Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide. Clinical and Experimental Immunology 2009, 156(3), 479-487.
  • Nile CJ, Read RC, Akil M, Duff GW, Wilson AG. Methylation status of a single CpG site in the IL6 promoter is related to IL6 messenger RNA levels and rheumatoid arthritis. Arthritis and Rheumatism 2008, 58(9), 2686-2693.
  • Nile CJ, Townes CL, Hirst BH, Hall J. The novel avian protein, AWAK, contains multiple domains with homology to protease inhibitory modules. Molecular Immunology 2006, 43(4), 388-394.
  • Townes CL, Michailidis G, Nile CJ, Hall J. Induction of cationic chicken liver-expressed antimicrobial peptide 2 in response to Salmonella enterica infection. Infection and Immunity 2004, 72(12), 6987-6993.
  • Nile CJ, Townes CL, Michailidis G, Hirst BH, Hall J. Identification of chicken lysozyme g2 and its expression in the intestine. Cellular and Molecular Life Sciences 2004, 61(21), 2760-2766.

• Conference Proceedings (inc. Abstracts)

  • Nile CJ, Barksby HE, Preshaw PM, Taylor JJ. The expression and regulation of IL-33 in human monocytes. In: Annual Congress of the British Society for Immunology. 2008, Glasgow, Scotland: Immunology: Wiley-Blackwell.
  • Townes CL, Nile CJ, Milona P, Hall J. The Kunitz domain of the novel avian protein, AWAK. In: FASEB Journal: Experimental Biology 2005 Meeting. 2005, San Diego, California, USA: Federation of American Societies for Experimental Biology.

• Reviews

  • Chuinsiri N, Edwards D, Telezhkin V, Nile CJ, Van der Cruyssen F, Durham J. Exploring the roles of neuropeptides in trigeminal neuropathic pain: A systematic review and narrative synthesis of animal studies. Archives of Oral Biology 2021, 130, 105247.
  • Delaney C, Kean R, Short B, Tumelty M, McLean W, Nile CJ, Ramage G. Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?. Current Clinical Microbiology Reports 2018, 5(3), 190-200.
  • O'Donnell LE, Millhouse E, Sherry L, Kean R, Malcolm J, Nile CJ, Ramage G. Polymicrobial Candida biofilms: friends and foe in the oral cavity. FEMS Yeast Research 2015, 15(7), fov077.
  • Zoheir N, Lappin DF, Nile CJ. Acetylcholine and the alpha 7 nicotinic receptor: A potential therapeutic target for the treatment of periodontal disease?. Inflammation Research 2012, 61(9), 915-926.