Staff Profile

Background

Parrs Wood High School, Manchester: 1977 - 1983

Medicine (MBBS) University of Newcastle upon Tyne Medical School: 1983-1988

Adult and Paediatric Medical training, Newcastle, Liverpool and London: 1988-1995

MRCP:1992

FRCPCH: 2006

British Heart Foundation Clinical Research Fellowship and PhD, University College London: 1995-1998

Cardiac and General Paediatric Intensive Care training, Great Ormond Street and Guy's Hospitals: 1998-2002

Consultant Paediatric Intensivist, Newcastle upon Tyne Hospitals NHS Trust: 2002-2009

Senior lecturer in cardiovascular development and disease: 2009-present

Links

ORCID iD: 0000-0003-2833-8882

European Society of Cardiology

Vice Chair of the Nucleus (committee) of the Working Group on Development Anatomy and Pathology.

Co-workers

Deborah Henderson, Professor of Cardiac Development

Louise Coats, Consultant in ACHD and NU Fellow

Lorraine Eley, BHF Research Associate

Chris Derrick, BHF Research Associate

Ahlam Alqahtani, BHF Research Associate

Mashael Alarady PhD student

Jasmine Turner PhD Student

Research themes

Cardiovascular developmental molecular biology and disease modelling using mice and zebrafish, asking:

What are the developmental origins of congenital heart disease?

How can we prevent people with genetic cardiomyopathies from progressing into heart failure?

How can we prevent late complications in adults with congenital heart disease?

Development of the aortic valve and Bicuspid valves

We are investigating how the aortic vavle forms and processes that lead to bicuspid aortic valve disease

Hypoplastic left heart syndrome

Hypoplastic Left Heart Syndrome (HLHS) is a rare but serious congenital heart malformation, in which there is severe underdevelopment of the left ventricle and aorta. We are looking for abnormalities in the genes of patients and combining this with our knowledge of how the heart forms, to discover if there is a genetic cause for HLHS.

Left-right signalling and planar cell polarity in cardiac development

Disturbances in the planar cell polarity pathway and in left-right specification cause heart malformations in mice and zebrafish. We are asking if these pathways are linked and if they may point to a genetic cause for malformations such as double outlet right ventricle and atrial/ventricular septal defects in people.

Remaining well with cardiomyopathyies

Whilst genetic cardiomyopathies cannot be prevented we can look for ways to help people with cardiomyopathy fto remain well and symptom free. We are exploring how biochemical stresses within cardiomyocytes can be controlled to prevent decompensation into heart failure and arrhythmia.

Helping adults with treated congenital heart disease to stay well

Children with congenital heart disease now survive into adulthood and are an expanding patient group. We are interested in understanding their issues, both medical and psychosocial, with the aim of maintaining their holistic wellbeing. We want to find ways to identify problems before they become clinically apparent and nvestigate how we can give this divers patient group the type and degree of support they need.

Technical expertise

• Laboratory models of genetic heart disease, including Crispr Cas9 genome editing in zebrafish and Cre-lox mice.
• Next Generation Sequencing (Whole exome and RNASeq) 
• Live imaging (including sheet light microscopy)
• Computer programming in biology (Unix, C, R, Matlab, Labview Arduino and RasperryPi)
• 3D reconstruction (Amira) and 3D printing (Reprap Ormerod).

My teaching responsibilities at Newcastle University are closely linked with my research activity. I provide undergraduate and postgraduate teaching cardiovascular development and congenital heart disease. In the laboratory I provide supervision for PhD, master’s and undergraduate students from our own biomedical sciences course and from other universities across the UK and abroad.

Genetics of Development and its Disorders (BGM3062)

Cardiovascular Science in Health and Disease (MMB8037)

• Murphy LB, Santos-Ledo A, Dhanaseelan T, Eley L, Burns D, Henderson DJ, Chaudhry B. Exercise, programmed cell death and exhaustion of cardiomyocyte proliferation in aging zebrafish. Disease Models and Mechanisms 2021, dmm.049013.
• Santos-Ledo A, Washer S, Dhanaseelan T, Eley L, Alqatani A, Chrystal PW, Papoutsi T, Henderson DJ, Chaudhry B. Alternative splicing of jnk1a in zebrafish determines first heart field ventricular cardiomyocyte numbers through modulation of hand2 expression. PLoS Genetics 2020, 16(5), e1008782.
• Alqahtani A, Skelton A, Eley L, Annavarapu S, Henderson DJ, Chaudhry B. Isolation and next generation sequencing of archival formalin-fixed DNA. Journal of Anatomy 2020, 237(3), 587-600.
• Henderson DJ, Eley L, Chaudhry B. New concepts in the development and malformation of the arterial valves. Journal of Cardiovascular Development and Disease 2020, 7(4), 1-27.
• Verykiou S, Alexander M, Edwards N, Plummer R, Chaudhry B, Lovat PE, Hill DS. Harnessing autophagy to overcome MEK-inhibitor induced resistance in metastatic melanoma. British Journal of Dermatology 2019, 180(2), 346-356.
• Eley L, Alqahtani A, MacGrogan D, Richardson R, Murphy L, Salguero-Jimenez A, Rodriguez San Pedro M, Tiurma S, McCutcheon L, Gilmore A, de La Pompa JL, Chaudhry B, Henderson D. A novel source of arterial valve cells linked to bicuspid aortic valve without raphe in mice. eLife 2018, 7, e34110.
• Richardson R, Eley L, Donald-Wilson C, Davis J, Curley N, Alqahtani A, Murphy L, Anderson RH, Henderson DJ, Chaudhry B. Development and maturation of the fibrous components of the arterial roots in the mouse heart. Journal of Anatomy 2018, 232(4), 554-567.
• Hill D, Chen L, Snaar-Jagalska E, Chaudhry B. Embryonic zebrafish xenograft assay of human cancer metastasis. F1000Research 2018, 7, 1682.
• Anderson RH, Henderson DJ, Chaudhry B. Development and maintenance of the arterial valves. European Heart Journal 2017, 38(9), 687-689.
• Piatek MJ, Henderson V, Fearn A, Chaudhry B, Werner A. Ectopically expressed Slc34a2a sense-antisense transcripts cause a cerebellar phenotype in zebrafish embryos depending on RNA complementarity and Dicer. PLoS ONE 2017, 12(5), e0178219.
• Crucean A, Alqahtani A, Barron DJ, Brawn WJ, Richardson RV, O'Sullivan J, Anderson RH, Henderson DJ, Chaudhry B. Re-evaluation of hypoplastic left heart syndrome from a developmental and morphological perspective. Orphanet Journal of Rare Diseases 2017, 12, 138.
• Anderson RH, Bamforth SD, Spicer DE, Henderson DJ, Chaudhry B, Brown NA, Mohun TJ. Development and maldevelopment of the ventricular outflow tracts. In: Lacour-Gayet F; Bove EL; Hraška V; Morell V; Spray T, ed. Surgery of Conotruncal Anomalies. Cham, Switzerland: Springer International Publishing, 2016, pp.27-59.
• Chaudhry B, Burns D, Murphy L, Henderson D. Exercise and cardiomyocyte turnover in the aging zebrafish heart. In: Third Congress of the ESC Council on Basic Cardiovascular Science: Frontiers in Cardiovascular Biology. 2014, Barcelona, Spain: Oxford University Press.
• Hill D, Verykiou S, Robinson N, Chaudhry B, Przyborski S, Lovat P. Modelling melanoma metastasis using organotypic skin equivalent and zebrafish models. In: British Society for Investigative Dermatology Annual Meeting. 2014, Newcastle, UK: Nature Publishing Group.
• Anderson RH, Mohun TJ, Spicer DE, Bamforth SD, Brown NA, Chaudhry B, Henderson DJ. Myths and Realities Relating to Development of the Arterial Valves. Journal of Cardiovascular Development and Disease 2014, 1(3), 177-200.
• Anderson RH, Moorman AFM, Brown NA, Bamforth SD, Chaudhry B, Henderson DJ, Mohun TJ. Normal and abnormal development of the heart. In: Pediatric and Congenital Cardiology, Cardiac Surgery and Intensive Care. London: Springer-Verlag, 2014, pp.151-177.
• Boczonadi V, Gillespie R, Keenan ID, Ramsbottom SA, Donald-Wilson C, Al Nazer M, Humbert P, Schwarz RJ, Chaudhry B, Henderson DJ. Scrib:Rac1 interactions are required for the morphogenesis of the ventricular myocardium. Cardiovascular Research 2014, 104(1), 103-115.
• Ramsbottom SA, Sharma V, Rhee HJ, Eley L, Phillips HM, Rigby HF, Dean C, Chaudhry B, Henderson DJ. Vangl2-Regulated Polarisation of Second Heart Field-Derived Cells Is Required for Outflow Tract Lengthening during Cardiac Development. PLoS Genetics 2014, 10(12), e1004871.
• Bamforth SD, Chaudhry B, Bennett M, Wilson R, Mohun TJ, van Mierop LHS, Henderson DJ, Anderson RH. Clarification of the identity of the mammalian fifth pharyngeal arch artery. Clinical Anatomy 2013, 26(2), 173-182.
• Phillips HM, Mahendran P, Singh E, Anderson RH, Chaudhry B, Henderson DJ. Neural crest cells are required for correct positioning of the developing outflow cushions and pattern the arterial valve leaflets. Cardiovascular Research 2013, 99(3), 452-460.
• Phillips HM, Papoutsi T, Soenen H, Ybot-Gonzalez P, Henderson DJ, Chaudhry B. Neural Crest Cell Survival is Dependent on Rho Kinase and is Required for Development of the Mid Face in Mouse Embryos. PLoS One 2012, 7(5), e37685.
• Anderson RH, Chaudhry B, Mohun TJ, Bamforth SD, Hoyland D, Phillips HM, Webb S, Moorman AF, Brown NA, Henderson DJ. Normal and abnormal development of the intrapericardial arterial trunks in humans and mice. Cardiovascular Research 2012, 95(1), 108-115.
• Keenan ID, Rhee HJ, Chaudhry B, Henderson DJ. Origin of non-cardiac endothelial cells from an Isl1+ lineage. FEBS Letters 2012, 586(13), 1790-1794.
• Henderson DJ, Chaudhry B. Getting to the Heart of Planar Cell Polarity Signaling. Birth Defects Research Part A: Clinical and Molecular Teratology 2011, 91(6), 460-467.
• Simms RJ, Hynes AM, Eley L, Inglis D, Chaudhry B, Dawe HR, Sayer JA. Modelling a ciliopathy: ahi1 knockdown in model systems reveals an essential role in brain, retinal and renal development. Cellular and Molecular Life Sciences 2011, 69(6), 993-1009.
• Taylor JM, Saunter CD, Love GD, Girkin JM, Henderson DJ, Chaudhry B. Real-time optical gating for three-dimensional beating heart imaging. Journal of Biomedical Optics 2011, 16(11), 116021.
• Anderson RH, Cook A, Brown NA, Henderson DJ, Chaudhry B, Mohun T. Development of the outflow tracts with reference to aortopulmonary windows and aortoventricular tunnels. Cardiology in the Young 2010, 20(s3), 92-99.
• Hammond KL, Loynes HE, Mowbray C, Runke G, Hammerschmidt M, Mullins MC, Hildreth V, Chaudhry B, Whitfield TT. A Late Role for bmp2b in the Morphogenesis of Semicircular Canal Ducts in the Zebrafish Inner Ear. PLoS One 2009, 4(2), e4368.
• Bradshaw L, Chaudhry B, Hildreth V, Webb S, Henderson DJ. Dual role for neural crest cells during outflow tract septation in the neural crest-deficient mutant Splotch(2H). Journal of Anatomy 2009, 214(2), 245-257.
• Hildreth V, Webb S, Chaudhry B, Peat JD, Phillips HM, Brown N, Anderson RH, Henderson DJ. Left cardiac isomerism in the Sonic hedgehog null mouse. Journal of Anatomy 2009, 214(6), 894-904.
• Chaudhry B, Ashton H, Muhamed A, Yost M, Bull S, Frankel D. Nanoscale viscoelastic properties of an aligned collagen scaffold. Journal of Materials Science: Materials in Medicine 2009, 20(1), 257-263.
• van't Padje S, Chaudhry B, Severijnen L, van der Linde H, Mientjes E, Oostra B, Willemsen R. Reduction in fragile X related 1 protein causes cardiomyopathy and muscular dystrophy in zebrafish. Journal of Experimental Biology 2009, 212(16), 2564-2570.
• Phillips HM, Hildreth V, Peat JD, Murdoch JN, Kobayashi K, Chaudhry B, Henderson DJ. Non-cell-autonomous roles for the planar cell polarity gene vangl2 in development of the coronary circulation. Circulation Research 2008, 102(5), 615-623.
• Phillips HM, Rhee HJ, Murdoch JN, Hildreth V, Peat JD, Anderson RH, Copp AJ, Chaudhry B, Henderson DJ. Disruption of planar cell polarity signaling results in congenital heart defects and cardiomyopathy attributable to early cardiomyocyte disorganization. Circulation Research 2007, 101(2), 137-145.
• Henderson DJ, Phillips HM, Chaudhry B. Vang-like 2 and noncanonical Wnt signaling in outflow tract development. Trends in Cardiovascular Medicine 2006, 16(2), 38-45.
• McKinney PA, Jones S, Parslow R, Davey N, Darowski M, Chaudhry B, Stack C, Parry G, Draper ES, PICANet Consent Study Grp. A feasibility study of signed consent for the collection of patient identifiable information for a national paediatric clinical audit database. British Medical Journal 2005, 330(7496), 877-879.
• Phillips HM, Murdoch JN, Chaudhry B, Copp AJ, Henderson DJ. Vangl2 acts via RhoA signaling to regulate polarized cell movements during development of the proximal outflow tract. Circulation Research 2005, 96(3), 292-299.
• Ioannides AS, Chaudhry B, Henderson DJ, Spitz L, Copp AJ. Dorsoventral patterning in oesophageal atresia with tracheo-oesophageal fistula: Evidence from a new mouse model. Journal of Pediatric Surgery 2002, 37(2), 185-191.