School of Natural and Environmental Sciences

Staff Profile

Sheralyn Smith

Biological Teaching Technician

Background

Qualifications:

BSc (Hons) Biological Sciences - (Animal Behaviour, Ecology and Conservation)

Memberships:

IST Membership MIScT - RSci (Registered Scientist)

Work Summary:

I work as a biological teaching technician supporting student undergraduate and postgraduate laboratory research projects.

Previous Experience:

Research Technician - Animal Science/Agriculture, Newcastle University

Animal Management Tutor - FE Animal Management Programme, Nottingham Trent University

Laboratory Technician - Animal and Environmental Sciences, Nottingham Trent University

Animal Welfare Rehoming Manager - RSPCA

Research Technician, Animal Behaviour and Welfare, Department of Veterinary Sciences, University of Bristol

Biology Technician - Anglia Ruskin University

 

Research

 

Instron Materials Testing Machine - Measuring chicken bone parameters and testing bone breaking strength.

Inductively Coupled Plasma - Measuring calcium and Phosphorous levels using ICP methodology. 

Mars 6 Microwave Technology - Acid digestion and solvent extraction of chicken bone material.

Elisa (EIA): Measuring 25-Hydroxy Vitamin D and Cortisol levels in chicken blood plasma samples.

RT-qPCR - Gene expression analysis on animal bone and intestinal tissue samples - nucleic acid extractions, CDNA synthesis and qPCR work.

Brookfield Viscometer DV2T – Measuring the viscosity in chicken intestinal digesta samples.

Spectrophotometer - Measuring biomarker levels of Titanium Dioxide in digesta samples spectrophotometrically.

PENTRA C400 Horiba Analysis – Investigating the levels of calcium and phosphorous in chicken blood samples.

Cube Analyser - Dumas Combustion methodology - levels of protein in digesta samples.

Parasitology Analysis - McMaster faecal nematode egg counts, Coccidial oocyst counts via centrifugation flotation method.

 

Histology – sample preparation; paraffin wax embedding, and microtome sectioning, H&E staining for measurements of tissue sections and goblet cells counts.

 

SDS PAGE – Protein separation using Polyacrylamide gel electrophoresis

 

Microbiology – Microbiology Techniques.

Publications