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Module

BIO2023 : Cellular Biochemistry

  • Offered for Year: 2020/21
  • Module Leader(s): Dr Fiona Cuskin
  • Lecturer: Dr Jon Marles-Wright, Professor Frank Sargent
  • Owning School: Natural and Environmental Sciences
  • Teaching Location: Newcastle City Campus
Semesters
Semester 1 Credit Value: 20
ECTS Credits: 10.0

Aims

To show how proteins are studied both within and outside of their host cells. To develop knowledge of the methods used to study protein structure and biochemistry. To develop skills in practical laboratory techniques related to protein isolation and characterisation, in the analysis of data using computer packages, and presentation and interpretation of protein biochemical and structural data.

Outline Of Syllabus

Lecture material
How we study proteins: structural biology, biophysical characterisation of proteins
Protein trafficking in the cell
Mitochondria: bioenergetics, biosynthesis and mitophagy
Enzyme mechanism and biological importance: Exemplified through the study of carbohydrate active proteins.

Practicals
•       Isolation of recombinant beta-galactosidase from Escherichia coli
•       Mechanism of beta-galactosidase activity
•       Competitive and non-competitive inhibition of enzyme activity

Workshops
•       Biochemical calculations
•       Protein structure analysis

Teaching Methods

Please note that module leaders are reviewing the module teaching and assessment methods for Semester 2 modules, in light of the Covid-19 restrictions. There may also be a few further changes to Semester 1 modules. Final information will be available by the end of August 2020 in for Semester 1 modules and the end of October 2020 for Semester 2 modules.

Teaching Activities
Category Activity Number Length Student Hours Comment
Structured Guided LearningLecture materials151:0015:00Online via Canvas - A combination of short recordings of lecture material and text published on VLE
Guided Independent StudyAssessment preparation and completion121:0021:00Revision and preparation of coursework (70%)
Guided Independent StudyAssessment preparation and completion115:0015:00Preparation of practical reports. (30%)
Guided Independent StudyAssessment preparation and completion25:0010:00Working through protein and biochemical workshop tasks
Guided Independent StudyDirected research and reading35:0015:00Online- Module lecturers will give information on directed reading.
Scheduled Learning And Teaching ActivitiesPractical33:009:00PiP - Students will be present in the laboratory for practical sessions
Scheduled Learning And Teaching ActivitiesWorkshops41:004:00Online- Protein workshop and biochemical workshop. For each, initial 1hr workshop with follow up 1hr
Scheduled Learning And Teaching ActivitiesDrop-in/surgery100:305:00Online- Q and A on lecture content.
Guided Independent StudyIndependent study1106:00106:00N/A
Total200:00
Teaching Rationale And Relationship

The online lectures explain key biochemical and biophysical methods and concepts, outline illustrative examples, and introduce the biochemical problems. The practical classes follow a logical structure from protein isolation to kinetic characterisation to allow students to develop skills in the use of laboratory equipment and analytical techniques and are designed to enhance students' understanding of enzyme characterisation and to enable them to increase their confidence in collecting, manipulating (including numerical calculations) and presenting data using computers. The biochemical problems develop students’ understanding of biochemistry and enzyme kinetics. Private study is necessary for students to absorb information presented in lectures, to deepen their knowledge and understanding through reading supporting references, to assess their progress through completion of formative on-line quizzes, to prepare their practical reports and to prepare for the biochemical problems.

Assessment Methods

Please note that module leaders are reviewing the module teaching and assessment methods for Semester 2 modules, in light of the Covid-19 restrictions. There may also be a few further changes to Semester 1 modules. Final information will be available by the end of August 2020 in for Semester 1 modules and the end of October 2020 for Semester 2 modules.

The format of resits will be determined by the Board of Examiners

Other Assessment
Description Semester When Set Percentage Comment
Practical/lab report1M30(3 sections, worth 10 % each)
Written exercise1M70Exam will be changed to Coursework Assessment consisting of a worked problem and a longer answer question.
Formative Assessments
Description Semester When Set Comment
Computer assessment1MOnline self-test quizzes for biochemical problems
Practical/lab report1MPractical report feedforward throughout course
Prob solv exercises1MBiochemical problems workshop
Written exercise1MIn-class test
Assessment Rationale And Relationship

Formative assessment
The biochemical problems workshop provides an opportunity to develop a working understanding of key mathematical concepts in biochemistry with worked examples and feedback.
Online self-test quizzes allow students to evaluate their progress and learning through the course. Biochemical problems assess numeracy and understanding of key concepts.

Summative assessment
Exam will be changed to a coursework Assessment consisting of a worked problem and a longer answer question.

Reading Lists

Timetable