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Module

BIO2023 : Cellular Biochemistry

  • Offered for Year: 2020/21
  • Module Leader(s): Dr Fiona Cuskin
  • Lecturer: Professor Frank Sargent, Dr Jon Marles-Wright
  • Owning School: Natural and Environmental Sciences
  • Teaching Location: Newcastle City Campus
Semesters
Semester 1 Credit Value: 20
ECTS Credits: 10.0

Aims

To show how proteins are studied both within and outside of their host cells. To develop knowledge of the methods used to study protein structure and biochemistry. To develop skills in practical laboratory techniques related to protein isolation and characterisation, in the analysis of data using computer packages, and presentation and interpretation of protein biochemical and structural data.

Outline Of Syllabus

Lecture material
How we study proteins: structural biology, biophysical characterisation of proteins
Protein trafficking in the cell
Mitochondria: bioenergetics, biosynthesis and mitophagy
Enzyme mechanism and biological importance: Exemplified through the study of carbohydrate active proteins.

Practicals
•       Isolation of recombinant beta-galactosidase from Escherichia coli
•       Mechanism of beta-galactosidase activity
•       Competitive and non-competitive inhibition of enzyme activity

Workshops
•       Biochemical calculations
•       Protein structure analysis

Teaching Methods

Module leaders are revising this content in light of the Covid 19 restrictions.
Revised and approved detail information will be available by 17 August.

Assessment Methods

Module leaders are revising this content in light of the Covid 19 restrictions.
Revised and approved detail information will be available by 17 August.

Reading Lists

Timetable