BIO2036 : Molecular Evolution and Systematics
- Offered for Year: 2021/22
- Module Leader(s): Professor John Bythell
- Lecturer: Dr Maria Del Carmen Montero-Calasanz, Dr Maxim Kapralov
- Owning School: Natural and Environmental Sciences
- Teaching Location: Newcastle City Campus
Semesters
| Semester 2 Credit Value: | 20 |
| ECTS Credits: | 10.0 |
Aims
This module provides a theoretical basis and practical understanding of molecular evolution, enabling students to ‘speak the language’ of modern genomic biology and undertake basic research to generate, access, manipulate and analyse molecular genetic data to address the systematics and phylogenetic relationships of organisms across the tree of life. Practical emphasis will be on molecular identification, classification and barcoding applications, including the Barcode Of Life Data (BOLD) programme. These techniques are also applicable to forensics and environmental DNA (eDNA) studies, where the presence of species are detected from environmental samples or unrecognisable remains. The concept of barcoding will also be expanded on, introducing the use of high-throughput next-generation sequencing (NGS) to characterise population or whole-community structure (metabarcoding or metagenomics).
Outline Of Syllabus
Lectures
Lectures will cover a range of subjects underlying molecular evolution, focussing particularly on the methods and interpretation of molecular data for characterisation, identification, systematics and phylogenetics. This will include:
- The molecular basis of biology and evolution
- Allele dynamics and populations
- DNA and amino acid sequence evolution
- DNA and protein sequencing technologies, applications and experimental design considerations
- Rates and patterns of molecular evolution
Lectures are supported by the recommended text (Graur 2016), which also provides a broader context.
Computer Lab Practicals and self-study exercises
Computer practicals are based on MEGA sequence analysis software and other freeware commonly used to analyse and manipulate DNA and protein sequences and build robust phylogenetic trees to support assessment of evolutionary relationships.
Laboratory Practicals
Cover all aspects of sample collection, DNA extraction, PCR amplification of barcoding markers and preparation of samples for sequencing.
Teaching Methods
Teaching Activities
| Category | Activity | Number | Length | Student Hours | Comment |
|---|---|---|---|---|---|
| Guided Independent Study | Assessment preparation and completion | 1 | 12:00 | 12:00 | Computer practical assessment (4 x exercises x 3hr each) |
| Guided Independent Study | Assessment preparation and completion | 1 | 46:00 | 46:00 | Revision plus exam (2hr) |
| Scheduled Learning And Teaching Activities | Lecture | 17 | 1:00 | 17:00 | N/A |
| Guided Independent Study | Directed research and reading | 4 | 1:00 | 4:00 | Preparation for computer practicals - Reading self-study guides |
| Guided Independent Study | Directed research and reading | 17 | 2:00 | 34:00 | Directed further reading (references provided in lectures) |
| Scheduled Learning And Teaching Activities | Practical | 4 | 3:00 | 12:00 | Computer cluster |
| Scheduled Learning And Teaching Activities | Practical | 3 | 3:00 | 9:00 | Laboratory practical - Ridley Labs A+B |
| Guided Independent Study | Directed research and reading | 3 | 1:00 | 3:00 | Prep for lab practicals - Reading lab protocols, H&S documents, further directed reading |
| Guided Independent Study | Skills practice | 3 | 3:00 | 9:00 | Follow up to lab practicals - Figure and table preparation, upload of materials to fileshare |
| Guided Independent Study | Reflective learning activity | 4 | 6:00 | 24:00 | Follow-up pylogenetics exercises - further computer exercises with model answers (formative) |
| Scheduled Learning And Teaching Activities | Drop-in/surgery | 1 | 1:00 | 1:00 | Revision Q+A session |
| Guided Independent Study | Independent study | 17 | 1:00 | 17:00 | Lecture follow up and consolidation |
| Guided Independent Study | Independent study | 1 | 12:00 | 12:00 | Independent study (research papers) |
| Total | 200:00 |
Teaching Rationale And Relationship
Lectures provide fundamental knowledge and understanding of the molecular basis of evolution and a broad awareness of the applications of modern analysis methods including next-generation sequencing. The skills to acquire, manipulate, analyse and present molecular sequence data are developed via computer-based practicals and associated follow-on exercises, providing formative assessment for subject-specific and generic skills development. Practical laboratory sessions develop laboratory skills in DNA extraction and PCR for phylogenetics analysis, using samples collected and identified from the field using morphological characteristics. Sequence data obtained in the laboratory are subject to phylogenetics analysis in an assessed computer practical. In combination, this provides a logical and coherent analysis from sample collection to phylogenetics analysis, with a real example to stimulate deep learning.
Assessment Methods
The format of resits will be determined by the Board of Examiners
Exams
| Description | Length | Semester | When Set | Percentage | Comment |
|---|---|---|---|---|---|
| Written Examination | 120 | 1 | A | 70 | N/A |
Other Assessment
| Description | Semester | When Set | Percentage | Comment |
|---|---|---|---|---|
| Practical/lab report | 2 | M | 30 | Computer practical assignment based on sequences obtained in the lab practical |
Formative Assessments
| Description | Semester | When Set | Comment |
|---|---|---|---|
| Practical/lab report | 2 | M | Follow-up additional exercises with model answers for each computer practical |
Assessment Rationale And Relationship
The written examination tests knowledge of the principles and practices of molecular evolution, molecular genetics and genomics technologies, as well as the ability to interpret the literature in this field. The exam consists of compulsory short-answer questions to test understanding across the subject, as well as a choice (1/3) of essay question to assess deeper understanding in a selected area and the ability to integrate material from wider reading. The skills to acquire, manipulate, analyse and present molecular sequence data are tested by means of an assessed computer laboratory report, undertaking a phylogenetics analysis on the organisms sequenced as part of the laboratory practical. The practical assessment includes elements of data acquisition from online databases, sequence alignment and phylogenetic tree construction, as well as assessment of the interpretation and presentation of information, including practical molecular lab results.
Reading Lists
Timetable
- Timetable Website: www.ncl.ac.uk/timetable/
- BIO2036's Timetable